Navigation
Open Access
Review

Interactions between bone cells and biomaterials: An update

Sabrina Beauvais1,Olivier Drevelle1,2,Jessica Jann1,Marc-Antoine Lauzon1,Mohammadreza Foruzanmehr3,Guillaume Grenier4,5,Sophie Roux5,6,Nathalie Faucheux1,5,*
1
Cells-Biomaterials Biohybrid Systems, Universite de Sherbrooke, Department of Chemical and Biotechnological Engineering, 2500, Universite Blvd, Sherbrooke, Quebec, Canada, J1K 2R1
2
Ecole Polytechnique de Montreal, 2900, Blvd Edouard Montpetit, Montreal, Quebec, Canada, H3C 3A7
3
Universite de Sherbrooke, Carrefour d’Innovations en Technologies Ecologiques (CITE), 2500, Universite Blvd, Sherbrooke, Quebec, Canada, J1K 2R1
4
Universite de Sherbrooke, Department of Surgery, 3001 12e Avenue N, Sherbrooke, Quebec, Canada, J1H 5N4
5
Centre de Recherche du CHUS, 3001 12e Avenue N, Sherbrooke, Quebec, J1H 5N4
6
Universite de Sherbrooke, Department of Medicine, 3001 12e Avenue N, Sherbrooke, Quebec, Canada, J1H 5N4
DOI: 10.2741/S460 Volume 8 Issue 2, pp.227-263
Published: 01 June 2016
*Corresponding Author(s):  
Nathalie Faucheux
E-mail:  
Nathalie.Faucheux@Usherbrooke.ca
Abstract

As the populations of the Western world become older, they will suffer more and more from bone defects related to osteoporosis (non-union fractures, vertebral damages), cancers (malignant osteolysis) and infections (osteomyelitis). Autografts are usually used to fill these defects, but they have several drawbacks such as morbidity at the donor site and the amount and quality of bone that can be harvested. Recent scientific milestones made in biomaterials development were shown to be promising to overcome these limitations. Cell interactions with biomaterials can be improved by adding at their surface functional groups such as adhesive peptides and/or growth factors. The development of such biomimetic materials able to control bone cell responses can only proceed if it is based on a sound understanding of bone cell behavior and regulation. This review focuses on bone physiology and the regulation of bone cell differentiation and function, and how the latest advances in biomimetic materials can be translated within promising clinical outcomes.

Key words

Alpha Integrin Subunit, Adhesion, Angiogenesis, Apoptosis, Beta Integrin Subunit, Biomaterial, Biomimetic, BMP, Bone, Bone Remodeling, Bone Substitute, Collagen, Differentiation, ECM, Extracellular Matrix, Fibrillar Adhesion, Fibronectin, Focal Adhesion, Focal Complex, Growth Factor, GTPase, Healing, Integrin, Interaction, Kinase, MAPK, Osteoblast, Osteoclast, Osteoinduction, Osteoconduction, Osteocyte, Peptide, Polymer, Proliferation, Receptor, RGD, Scaffold, Signal Transduction, Smad, TGF, Vascularization, VEGF, Wnt, Review

2. Introduction

People over 65 years old make up the most rapidly growing Canadian population (1). Five million Canadians were over 65 years old in 2011, and this number is estimated to double by 2036, so that they will account for a quarter of the population (2,3). The World Health Organization (WHO) estimates that the worldwide population aged over 60 will reach 2 billion by 2050 (4). These older people are most at risk of bone disorders like secondary metastasis causing bone cancer or osteoporotic fractures which are leading causes of pain and disability (5). Osteoporosis Canada reports that over 80 percent of the fractures sustained by people over 50 years old are caused by osteoporosis (6). Major bone loss associated with osteoporosis (particularly in vertebral fractures or non-union fractures) can be difficult to repair, so becoming a significant financial burden. The annual cost to Canadians of treating osteoporosis and the resulting fractures could reach 30 billion dollars by 2018 (7).

Bone is the basic support of locomotion system, where muscles, ligaments and tendons are attached to it. It also provides mechanical support and protects vital organs. It contains bone marrow, the main site of hematopoiesis and an important reservoir of minerals (8,9). Osteointegrative, osteoconductive and osteoinductive biocompatible bone substitutes should, ideally, be used to heal fractures and bridge bone losses (8). Osteointegration requires extensive interaction between the bone substitute and the recipient’s bone site. Osteoconduction can only occur if the bone substitute is readily colonized by bone cells and blood vessels, while osteoinductive substitute must be able to stimulate host’s mesenchymal stem cells (MSCs) from surrounding tissues to differentiate into bone-forming cells (10).

Biological graft such as autografts, the gold standard used by surgeons, and allografts are the most commonly used (10). Autografts use patient’s own living bone tissue, while allografts use cadaveric bone. Autografts provide an osteoconductive scaffold, osteogenic cells and osteoinductive growth factors, while allografts have a limited range of these properties (10). However, the size of an autograft is limited by the amount of bone that can be harvested, and 8.5 to 20 percent of cases suffer from postoperative complications like nerve injury, infections, blood loss, morbidity and chronic pain at the donor and/or recipient’s sites (10,11). Most grafts are harvested from iliac crests so as to limit structural modification, but their size may vary considerably (5 to 70 cm2) (12,13). Larger grafts can be taken from the tibia or femur, providing generally greater quantities of osteogenic growth factors such as bone morphogenetic proteins (BMPs) (13). These donor sites do not provide horseshoe shaped grafts consisting of cortical bone (tricortical graft) that give greater structural support and mechanical resistance, unlike the iliac crest. In contrast to autograft bone, allograft is frozen in liquid nitrogen and undergoes a serie of treatments such as purification (NaHCO3, H2O2, NaOH), conditioning and sterilization. These treatments have a considerable effect on the biological and mechanical properties of bone tissue (1416). With modern procurement and sterilization methods for bone tissue, the risk of infection transmission by allografts such as human immunodeficiency virus is estimated to be 1 in 2.8 billion (17,18).

Biomaterials with the same characteristics as bone grafts have to be developed to overcome these problems (limited graft size, morbidity and chronic pain) (19). The biomaterial may be inorganic, like hydroxyapatite (HAP) or titanium (Ti), natural, such as collagen or alginate, or synthetic polymer like polycaprolactone (PCL), polylactate, or even a composite material (8). Inorganic biomaterials like calcium phosphate ceramics have the same physical properties as bone mineral and induce a minimal immune response in vivo during implantation. However, the solubility of the calcium phosphate ceramics influences the activity of osteoclasts, the cells responsible for bone resorption (19,20). Other materials, including synthetic polymers, can be broken down over time and replaced by regenerated tissue in the long term (19). However, they interact poorly with bone cells, resulting in the development of third generation biomaterials, such as biomimetic materials (8,21,22).

Several strategies have been used to create biomimetic materials. One of them is to functionalize the biomaterial by adding extracellular matrix (ECM) proteins, recombinant growth factors and/or peptides derived from them in order to mimic bone as close as possible to its physiology (2328). The therapeutic potential of these biomimetic materials depends on their capacity to control the behavior of MSCs (29). Biomimetic materials must first promote the adhesion of MSCs to their surface and favor the response of these cells to specific growth factors leading to their differentiation into bone-forming cells. The interactions between cell membrane receptor integrins and the proteins/peptides at the surface of the biomimetic material play a crucial role in this phenomenon (30,31).

In the present review, we first describe the principal components of bone and their roles in bone healing and remodeling. We then look at the adhesion of bone cells to ECM and biomaterials and the crucial role of cell-biomaterial interactions in the integration and repair of bone tissue. Finally, the roles of each of these elements will be set in the context of the latest advances in the field of biomimetic materials.

3. Bone cells and the extracellular matrix

3.1. Bone cells

Bone remodeling is a physiological process in which bone resorption is followed by the formation of new bone. The cells responsible for these interrelated processes include the bone-resorbing cells, i.e.osteoclasts, which are derived from hematopoietic cells of the monocyte-macrophage lineage, and bone-forming cells, i.e.osteoblasts, which differentiate from bone marrow MSCs. Osteoblasts, osteoblast-derived osteocytes and osteoclasts are highly specialized bone cells (32).

Mature osteoblasts have a lifespan of about 3 months, and are protein-secreting cells with a well-developed rough endoplasmic reticulum and a large Golgi apparatus (33). They synthesize the collagen-based matrix, the osteoid, at a rate of 2 to 3 microns3 per day and mineralize it 10 days after its deposition. As a result, osteoblasts become surrounded by mineralized tissue. Osteoblasts that cover the bone surface become inactive lining cells, or they die by apoptosis. Osteoblasts can control the differentiation of bone resorbing osteoclasts by secreting the receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG) (34).

Osteocytes make up 90 to 95 percent of all bone cells and have a half-life of about 25 years (35). They originate from osteoblasts once they have become enclosed within the mineralized tissue. They are interconnected together via adherent and gap junctions. The osteocytes are sensors of bone mechanical stimuli and the microdamage induced by cyclic loading. They may also respond to fluid flow induced by strain in the canaliculi (36). Osteocytes secrete major bone-regulating factors. They are the main source of RANKL in skeletal tissue, and also produce OPG and sclerostin, which influence the activity of other specialized bone cells (37). Bone microdamages may also cause osteocytes to enter apoptosis, which favors the release of chemotactic signals that target osteoclasts (38).

Osteoclasts are large multinucleated cells with diameter about 50 to 100 microns formed by the fusion of monocytes, which are mononuclear cells. Osteoclasts have a lifespan of about 2 weeks. They become activated when they attached to the bone matrix (33,39). Osteoclasts are highly motile and alternate between migratory and bone-resorbing stages, showing remarkable changes in their phenotype during these phases. When adhered to the bone, the osteoclasts become polarized and reorganize their cytoskeleton. A sealing zone is formed by densely packed actin-rich podosomes that delimit the ruffled border, a highly specialized area composed of membrane expansions directed toward the targeted bone surface. The ruffled border is formed by polarized vesicular trafficking and plays a critical role in the degradation of bone matrix through acidification by vacuolar H+ ATPases and degrading enzymes released by the fusion of secretory lysosomal vesicles such as matrix metalloproteinases (MMP) and cathepsin K, or acid phosphatases such as tartrate resistant acid phosphatase (TRAP). These proteases can degrade the mineralized osteoid to form Howship lacunae 40 to 60 microns deep (40). Osteoclasts also transport vesicles from basal to the apical cell membrane by transcytosis that contain calcium and phosphate ions and hydrolyzed osteoid proteins released during bone resorption (41). In non-resorptive or migrating osteoclasts, the sealing zone switches to a podosome belt, and relaxed osteoclasts are depolarized (42).

3.2. Osteoid and mineral phase

Osteoid accounts for 20 to 25 percent of the bone mass. It is made up of over 90 percent of type I collagen (43,44). Collagen fibrils (15 to 500 nm in diameter) are stabilized by intramolecular and intermolecular crosslinks formed by covalent, electrostatic and hydrogen bonds (45). Collagen fibrils can co-assemble to form collagen fibers of about 10 microns in diameter (45). The structure and the organization of collagens influence the mechanical properties of bone, such as its ductility and fracture resistance (46).

Osteoid matrix also contains around 5 percent of non-collagenous proteins. These are proteins like the small integrin-binding ligand N-linked glycoproteins (SIBLING), such as osteopontin (OPN), matrix extracellular phosphoglycoprotein (MEPE) and bone sialoprotein (BSP) (47). SIBLING proteins undergo extensive post-translational modifications, including N/O-linked glycosylation, sulfation and/or phosphorylation, that influence their function (for review see (48)). They are important in the regulation of bone cell function and matrix mineralization, acting via their RGD sites and their acidic serine-rich and aspartate-rich motif (ASARM) (4851). Holm et al. recently found that the trabecular bones of BSP-/- knockout mice were less well mineralized than those of their wild type controls (52).

Adhesive proteins like fibronectin are also crucial for bone: they interact with cells via integrins to regulate their activity (53,54). Schwab et al. recently reported that fibronectin was better than vitronectin for the adherence of human bone marrow MSCs and their differentiation into osteoblasts (53).

Osteoid also contains the small leucine-rich proteoglycans (SLRPs), biglycan and decorin, whose central protein cores are linked by glycosaminoglycans such as chondroitin sulfate (55). Ingram et al. showed that biglycan was present in both cortical and trabecular bone, while decorin was mainly located in the canaliculi of osteocytes and in the matrix near the Haversian canals (55). The collagen fibrils are abnormal in decorin-/- and biglycan-/- double-knockout mice (56). SLRPs can regulate the hydrostatic and osmotic pressures as well as the transport of nutrients and growth factors (57). Chen et al. used neonatal murine calvarial cells extracted from biglycan-/- knockout mice and adenovirus encoding biglycan to show that this proteoglycan was required for the osteogenic differentiation of calvarial cells induced by BMP-4 (58). SLRPs also play a key role in regulating the mechanical properties of bone, especially its poroelasticity (57).

The mineral part of bone is almost entirely made of HAP crystals, constituted of calcium and phosphate ions. It accounts roughly for 65 percent of the bone mass (59). It also contains other ions - fluoride, manganese and magnesium - with some carbonate substitution. Mineralization of the osteoid begins with the nucleation of calcium phosphate followed by crystal growth (60). Nucleation can develop from supersaturated concentrations of calcium and phosphate ions. It occurs in specific vesicles that bud off from the membranes of hypertrophic chondrocytes and osteoblasts and in the interstitial space through the action of specific SIBLING proteins (60). The apatite crystals first form in the gaps between the collagen molecules. Several recent in vitro studies have suggested that collagen itself has specific sites rich in charged amino acid that favor crystal nucleation (6163). Wang et al. found that collagen could sequester enough calcium, phosphate and carbonate ions to favor their spontaneous transformation into apatite crystals (64). However, there is still some debate about the results of these studies because most of them used tendon or isolated collagen fibrils as models, which lack the SIBLING proteins.

4. Bone cell differentiation and bone healing

4.1. Signaling pathways contributing to osteogenic differentiation of mesenchymal stem cells

The use of MSCs in tissue engineering is a promising strategy to enhance bone healing and regeneration (65). However, fundamental understanding of the osteoblastic commitment capacity of implanted MSCs and its regulation will be critical (65). The differentiation of MSCs into osteoblasts, chondrocytes, and adipocytes is regulated by growth factors, cytokines, hormones and vitamins (Figure 1) (66).

Many growth factors, including fibroblast growth factor (FGF), insulin-like growth factor (IGF), the transforming growth factor-beta family (TGF-beta) and the platelet-derived growth factor (PDGF), are involved in the differentiation of MSCs into osteoblasts (6770). For example, Baker et al. found that bone ossification was abnormally slow in IGF-1-/- mice (68).

Figure 1. Signaling pathways regulating the differentiation of MSCs into osteoblasts (ALP, alkaline phosphatase; CSL, CBF1 Suppressor of Hairless Lag-1; EphB4, Ephrin type-B receptor 4; Hey, Hes-related with the YRPW motif; MAML, Mastermind-like; NICD, Notch intracellular domain; PI3K, phosphatidyl inositol 3 kinase; Sema4D, semaphorin 4D; VEGF, vascular endothelial growth factor (7182). [Illustration using Servier Medical Art, http://www.servier.fr].

FGF and IGF bind to receptors belonging to the tyrosine kinase receptor family, FGF receptor (FGFR) for FGF and IGF type I receptor (IGFIR) for IGF. The receptors bearing their growth factors form dimers that are activated by trans-phosphorylation of their tyrosine residues. These receptors then recruit intracellular adaptor proteins such as growth factor receptor-bound protein 2 (Grb2) and (Src homology 2 domain-containing)-transforming protein (Shc). The son of sevenless (Sos) is then recruited and the extracellular signal regulated kinase 1/2 (ERK1/2) mitogen activated protein kinase (MAPK) cascade is activated. This cascade can trigger the differentiation of MSCs into osteoblasts (83,84).

The BMPs also play a crucial part in bone tissue formation. More than 20 BMPs have been identified to date (23,29). These molecules are synthesized by the MSCs and osteoblasts and are members of the TGF-beta family (85,86). Marshall Urist showed that implanting demineralized bone in a muscle led to de novo bone formation. He also discovered that BMPs gave the organic bone matrix its osteoinductive properties (87). Each kg of demineralized bone matrix contains 1 – 2 microg BMPs (8890). The osteogenic potential of BMPs has been verified in vivo by injecting C2C12 cells transformed with adenovirus encoding BMPs (AdBMP) into mouse quadriceps muscles (91). Kang et al. found that AdBMP-6 or AdBMP-9 triggered ossification most rapidly and efficiently, followed by AdBMP-2 and AdBMP-7 (91). Like other members of the TGF-beta family, BMPs act on cells by inducing two type I and two type II serine/threonine kinase receptors to form a heterotetrameric complex. A total of 7 type I receptors and 5 type II receptors have been identified to date. They can bind over 30 TGF-beta family ligands and all have similar structures (92). For example, the kinase domains of the BMP type I receptors (BMPR) BMPR-IA and BMPR-IB share 85 percent amino acids homology (92).

There are two pathways involved in BMP signaling, the canonical Smad pathway and a pathway involving TGF-beta activated tyrosine kinase 1 (TAK1) and MAPK (93). In the canonical Smad pathway, after BMPs binding to receptors, the type I receptor is phosphorylated by the type II receptor, which in turn leads to the phosphorylation of Smad1/5/8 (94). The phosphorylated Smad1/5/8 then form a complex with Smad4. This complex is translocated to the nucleus, where it activates the transcription of osteogenic genes like Runx2, osterix (Osx) and osteocalcin (OC) (9496). Liu et al. reported that small interfering RNA (siRNA) against Smad1 reduced the amount of ALP mRNA induced by BMP-2 in MC3T3-E1 preosteoblasts and inhibited matrix mineralization (97).

The canonical Smad pathway is regulated at many levels (Figure 2). The number of available BMP receptors at the cell surface can be modulated by endocytosis. Extracellular regulation occurs when antagonists such as Noggin, Chordin and Gremlin bind to BMPs and inhibit their interaction with their receptors (98100). The Smad pathway is also regulated by the transmembrane pseudoreceptor BMP and activin membrane-bound inhibitor (BAMBI), which interacts with BMPRI to prevent the transduction of the signal (101). The inhibitory Smads (I-Smad), Smad6 and Smad7, are intracellular regulators of the Smad pathway. They bind to the intracellular domain of type I receptors to form a stable complex that prevents the activation of Smad1/5/8 (102,103). Other intracellular regulators of the Smad pathway are the phosphatases. Protein phosphatase, Mg2+/Mn2+ dependent, 1A (PPM1A) dephosphorylates Smad1 and inhibits its BMP-2-induced transcriptional activity (104).

Figure 2. Regulation of BMP-induced signaling (93,94,97,100,101,104,106,109112). [Illustration using Servier Medical Art, http://www.servier.fr].

The interaction of a BMP with its receptors can also activate the MAPK signaling pathway (Figure 2). The MAPK pathway is divided into 3 cascades: ERK1/2, p38 and c-jun N-terminal kinase (JNK). The BMPs facilitate the recruitment of a MAPKKK, TAK1, to the type I receptor and then the activation of the 3 MAPK cascades (93). The mechanism by which TAK1 is activated by type I receptors is still unknown. Perhaps the X-linked inhibitor of apoptosis (XIAP) mediates the signal transduction between BMP receptor and TAK1 (105). The phosphorylated ERK1/2, p38 and JNK are then translocated to the nucleus, where they interact with the factors controlling the transcription of specific genes (106). ERK1/2, p38 and JNK can have either positive or negative effect on osteoblastic differentiation. Xu et al. showed that inhibiting p38 decreased the synthesis of ALP by C3H10T1/2 cells infected with AdBMP-9 (107). Similarly, Lauzon et al. found that the inhibition of either JNK or ERK1/2 increased ALP activity in MC3T3-E1 preosteoblasts stimulated with BMP-9 or BMP-2 in the presence of fetal bovine serum (108). Others have found that ERK1/2 regulates the Smad canonical pathway by phosphorylating Smad1 at the linker region, so preventing the complex formed by Smad1 and Smad4 from being translocated to the nucleus (109).

BMPs and other growth factors can act in synergy to trigger the differentiation of MSCs or bone cells. Lauzon et al. showed that IGF-2 increased the BMP-9-induced ALP activity in MC3T3-E1 cells (108). FGF2 (or bFGF) is also involved in osteogenic differentiation through its action on the concentration of BMP-2. The concentration of BMP-2 in FGF2-/- mice is drastically decreased leading to reduced bone formation (113). Epidermal growth factor (EGF) also enhances the ALP activity in immortalized mouse embryonic fibroblasts that have been infected with AdBMP-9 (114).

The canonical Wnt/beta-catenin signaling pathways, Notch and Hedgehog (Hh) are also crucial for the differentiation of MSCs into osteoblastic lineage (Figure 3) (115,116). The Wnt/beta-catenin pathway is important for determining the fate of stem cells; it not only favors osteogenic differentiation, it also inhibits adipogenic differentiation (117). Canonical Wnt agonists act on cells by binding to its receptor Frizzled (Fzd) and its co-receptors low density lipoprotein receptor related protein 5 and 6 (LRP5/6). This leads to the recruitment of the Dishevelled (Dvl) protein, which inhibits the phosphorylation of the beta-catenin by glycogen synthase kinase-3 beta (GSK-3 beta) and its subsequent ubiquitination and degradation by proteasomes. The unphosphorylated beta-catenin can then translocate to the nucleus and interact with the transcription factor T-cell factor/lymphoid enhancer factor (TCF/LEF). This complex enables the transcriptional activity of genes encoding proteins like Runx2 and BMP-2 (118120). Tang et al. showed that C3H10T1/2 cells infected with AdWnt-3a increased ALP activity and enhanced the ALP activity induced by AdBMP-9 (121).

Figure 3. Crosstalk between Wnt and growth factors that regulate osteoblast behavior (APC, adenomatous polyposis coli; Dkk-1, Dickkopf-1; GFs, growth factors; STKR, serine/threonine kinase receptor) (93,94,106,109,119,122127). [Illustration using Servier Medical Art, http://www.servier.fr].

Notch is a transmembrane receptor that interacts with the ligands Delta or Jagged present on the surface of neighboring cells. Notch intracellular domain (NICD) is then cleaved by gamma-secretase and moves to the nucleus, where it binds to transcription factors like CBF1, suppressor of hairless (CSL) and the co-activator Mastermind-like (MAML) to stimulate the transcription of genes encoding Hairy enhancer of split (Hes) and Hey (124). Ugarte et al. showed that activating the Notch pathway in human MSCs by causing them to overproduce Jagged1 or NICD, induced mineralization and increased their ALP activity and BMP-2 expression, while inhibiting their differentiation into adipocytes (128). However, activating the Notch pathway does not stimulate OC synthesis, which suggests that the Notch pathway induces early osteogenic differentiation but not the formation of mature osteoblasts. The transcription factors like Hes and Hey, which are Notch targeted genes, also influence the responses of cells to BMPs. Sharff et al. observed that silencing Hey1 in C3H10T1/2 cells reduced their BMP-9-mediated ALP activity. Infecting the cells with AdRunx2 caused the ALP activity to recover (129). BMP-2 also triggers C2C12 cells to undergo osteogenic differentiation by increasing the expression of genes encoding for ALP and OC, together with increased expression of gene encoding for Hey1 and decreased Hes1 transcripts (130). However, Zamurovic et al. found that Hey1 antagonized the transcriptional activity of Runx2 in MC3T3-E1 preosteoblasts stimulated by BMP-2, which led to decreased mineralization (131).

Hedgehog (Hh) is also involved in osteogenic differentiation. It binds to its receptor Patched at the cell surface, which prevents Patched from inhibiting the transmembrane protein Smoothened. This enables Smoothened to activate a signaling cascade leading to stabilization of the transcription factor Gli2. The newly-stabilized Gli2 then activates the transcription of target genes such as that encoding Gli1, which is also a transcription factor that promotes the expression of genes like those encoding ALP and BSP (124,132). The effect of Hh on differentiation depends on the species. Plaisant et al. observed that conditioned medium from sonic Hh-secreting cells inhibits the synthesis of ALP, Runx2, osteonectin and OPG by human multipotent stem cells derived from adipose-tissue (122). There is also a crosstalk between Hh and the Wnt pathway. Hu et al. showed that inhibiting the Wnt pathway in C3H10T1/2 cells by transfecting the cells with retrovirus encoding Dkk-1 decreased the ALP production induced by a constituvely active Smoothened protein (125). Spinella-Jaegle et al. found that stimulating C3H10T1/2 cells with sonic Hh increased the ALP activity induced by BMP-2. However, sonic Hh had no effect on the ALP activity in MC3T3-E1 preosteoblasts induced by BMP-2 (123).

4.2. Osteoclastogenesis

Osteoclastogenesis involves the commitment of hematopoietic precursor cells to the monocyte/macrophage lineage, the fusion of several precursors and their transformation into mature osteoclasts (32). These processes are regulated by two major signaling pathways that are activated by macrophage colony-stimulating factor (M-CSF) and RANKL, a member of the tumor necrosis factor (TNF) ligand superfamily. M-CSF promotes RANK expression and mediates the proliferation of osteoclast precursors and their differentiation and survival. RANKL is crucial for osteoclast differentiation, survival and bone-resorbing activity (Figure 4) (133135). RANKL also favors the retention of the osteoclast precursors in bone by down-regulating the gene encoding the receptor S1PR1 of the lipid mediator sphingosine-1 phosphate (SP-1), which favors the passage of osteoclast precursors from the bone to blood vessels (136). M-CSF and RANKL are synthesized by osteoblasts, osteocytes, bone marrow stromal cells and lymphocytes in response to stimulation by factors including hormones (parathyroid hormone (PTH), vitamins D), inflammatory cytokines (Interleukin-1 (IL-1), IL-6, TNF alpha, interferon-gamma, IFN gamma) (137140).

Figure 4. Osteoclast structure and regulation by cytokines and growth factors (AP-1, activating protein-1; PLCgamma, phospholipase C gamma; TRAP, tartrate resistant acid phosphatase) (32,72,140,144,148,149,154156). [Illustration using Servier Medical Art, http://www.servier.fr].

M-CSF binds to the tyrosine kinase receptor c-Fms on osteoclast precursors and causes activated c-Fms to form dimers. These become phosphorylated on their multiple tyrosine residues, enabling them to interact with proteins containing SH2 domains like Grb2 (Y697, Y974), c-Src (Y559), PI3K (Y721) and to transduce intracellular signaling of M-CSF. PI3K then stimulates Akt, while Grb2 activates the Ras/Raf/MEK/ERK pathways by interacting with Sos (for review see (141,142)). These pathways are mainly involved in the proliferation and survival of osteoclast precursors. However, Amano et al. recently demonstrated that the M-CSF-triggered differentiation of murine 4B12 precursor cells (Mac-1(+) c-Fms (+) RANK (+) cells from calvaria of 14-day-old mouse embryos) into osteoclasts also depended on the activation of ERK5 (143).

RANKL acts on osteoclast precursors by binding to its RANK receptor, which, in turn, allows the binding of TNF receptor associated factor-6 (TRAF6) to the intracellular domain of RANK. It induces the activation of several signaling pathways including NF-kappaB, MAP kinases (JNK, ERK1/2, and p38), and also leads to the mobilization of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway (32,144). RANKL may also activate the calcium signals that lead to the activation of another major transcription factor, nuclear factor of activated T cells cytoplasmic 1 (NFATc1), through an immunoreceptor tyrosine-based activation motif-mediated co-stimulatory signaling (145). RANKL-RANK binding induces the hydrolysis of phosphatidylinositol 4,5-bisphosphate by phospholipase C to give inositol-1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (for review see (146)). The subsequent release of calcium ions from the endoplasmic reticulum induced by IP3 activates calcineurin, which then stimulates NFATc1 by dephosphorylating it (147). NFATc1 activates genes encoding proteins essential for osteoclastogenesis, such as dendritic cell-specific transmembrane protein (DC-STAMP), which is required for cell fusion (148). The activation of NFATc1 by RANKL is amplified by stimulating osteoclast precursors with immunoglobulin-like receptor ligands that bind triggering receptor expressed in myeloid cells 2 (TREM-2) or osteoclast-associated receptors (OSCAR). These receptors become activated by associating with adaptor proteins containing the immunoreceptor tyrosine-based activation motif (ITAM). The ITAM-mediated signal from TREM-2 involves the 12 kDa DNAX-activating protein (DAP12), while OSCAR involves the Fc receptor common gamma (149,150). The adaptors recruit the protein tyrosine kinase Syk, and the resulting signal leads to the release of calcium and activation of transcription factors like NFAT and NF-kappaB. Barrow et al. recently reported that OSCAR can bind strongly to type I collagen (151). This interaction promotes osteoclastogenesis (151). The effects of pro-osteoclastogenic factors may also depend on signaling modulators. The recombinant recognition sequence binding protein at the Jkappa site (RBP-J) is a novel transcription factor which inhibits osteoclastogenesis by imposing a requirement of ITAM-mediated co-stimulation for TNF alpha and RANKL signaling (152). Activating RBP-J in osteoclast precursors suppresses the osteoclastogenesis induced by inflammatory stimuli, such as TNF alpha. Conversely, inactivation of RBP-J considerably increases RANKL-independent osteoclastogenesis (153). Finally, the fine-tuning of bone resorption also involves OPG, a secreted decoy RANKL receptor that competes with RANK and inhibits osteoclast differentiation and functions. RANKL signaling can be completely blocked by sequestering RANKL using OPG (32,144). It is also inhibited by cytokines such as IFN gamma (140).

Osteoclastogenesis is also regulated by BMPs like BMP-2 (157159). Thus, the differentiation of bone marrow cells into osteoclasts requires less RANKL (half optimal dose) when BMP-2 is present in the culture medium (159). The extracellular inhibitor of BMP-2, Noggin, severely impairs the osteoclastogenesis induced by RANKL and the BMP type II receptors BMPRII is required for osteoclastogenesis in vitro (157,159). Using human mononuclear leukocyte suspensions isolated from umbilical cord blood, Fong et al. showed that BMP-9 could protect osteoclasts against apoptosis via a decrease in caspase-9 activation (160). BMP-9 also enhanced the bone resorption by mature osteoclasts (160).

4.3. Bone healing

Bone healing can be divided into two processes, one not excluding the other: intramembranous and endochondral healing. Intramembranous process occurs in fractures that heal by first intention. Fracture sites should have no defect and should be mechanically stable (described in more details in the next section). The healing of larger bone fractures involves both endochondral bone formation and intramembranous healing. Endochondral healing (second intention) occurs in four phases: inflammation and hematoma formation, fibrocartilagenous callus formation, bony callus formation, and finally bone remodeling (for review see (161)). The periosteum is important for bone healing especially during endochondral healing since it provides chondrogenic lineage precursor cells (162). Each phase requires different cytokines, growth factors and vitamins. Growth factors like BMP, TGF-beta, IGF, FGF, PDGF and VEGF all are involved in bone repair (Figure 5) (163). The PTH is also important in bone healing process since it can enhance bone formation by increasing cartilage volume as well as bone mineral density (for review see (161)).

Figure 5. Endochondral healing process following bone fracture (161,163167). [Illustration using Servier Medical Art, http://www.servier.fr].

Several types of cells are also involved in the endochondral bone healing. Macrophages are recruited during hematoma formation/inflammation to remove damaged cells and tissue. They secrete cytokines that foster the recruitment (infiltration) and stimulation of leukocytes to the injury site (168,169). Prostaglandin E2, which is present at the fracture site, can also recruit T lymphocytes during this step (170). The second phase of bone healing is the formation of the fibrocartilagenous callus. The chondrogenic lineage precursor cells in the periosteum move to the injury site, where they differentiate into mature chondrocytes under the influence of BMP-2, which is present throughout bone healing (167). Other cytokines and growth factors also promote the differentiation of the precursor cells into mature chondrocytes. TGF-beta3 also facilitates the differentiation of stem cells into chondrogenic lineage (TGF-beta3-BMP2 synergy) (171). FGF-2 can enhance and control the proliferation of the chondrocytes and osteoblast progenitors during each of the bone healing step (165). The transition between the fibrocartilagenous and bony callus phases is promoted by angiogenesis and the differentiation of precursor cells into mature osteoblasts. VEGF and angiopoietin are involved in the vascularization of the callus and the final bone formation/mineralization (161).

Finally, the last stage of bone healing is bone remodeling, which can be subdivided in five distinct phases: quiescence, preosteoclast recruitment and osteoclast differentiation, bone resorption, preosteoblast recruitment and osteoblast differentiation which ultimately lead to the formation of structured bone. The first step is osteoclastogenesis and two hypotheses have been advanced to explain osteoclast activation. The first is that osteoclastogenesis is activated by the apoptotic bodies of the osteocytes while the second is that the death of osteocytes leads to a reduction in the amount of TGF-beta (172174). The activated osteoclasts then start to resorb bone (155,156). Resorption takes up to 30 days, while bone formation and the recruitment of osteoblasts and osteoblast precursors can take 150 days. The cell signaling that triggers the passage from bone resorption to formation is not yet fully understood. Mature osteoblasts then synthesize the bone matrix and allow its mineralization.

The various cells involved in bone remodeling communicate with each other in several ways throughout the process. Osteoclasts synthesize Sema4D that inhibits the formation of the bone matrix by osteoblasts (72). Sema4D binds to the Plexin-B1 receptors on the osteoblasts and these then activate RhoA to prevent the matrix mineralization induced by IGF-1 (72).

5. Cell adhesions

5.1. Types of cell adhesions

Interactions between cells and ECM or biomaterials are essential for tissue integrity and repair. They influence cell survival, proliferation, differentiation and migration. The integrins are the main heterodimeric alpha beta transmembrane glycoproteins cell receptors involved in the adhesion of cells to ECM proteins and biomaterials (175). There are presently 18 alpha and 8 beta subunits that assemble to form 24 distinct integrins. They interact with adhesive proteins from the ECM via specific amino acid sequences. The most common site on ECM proteins to which integrins bind is the tripeptide sequence Arg-Gly-Asp (RGD). It is found on type I collagen, OPN, BSP, thrombospondin and fibronectin (176). Alphav beta3 integrins bind to several proteins, including fibronectin, vitronectin and BSP, while alpha5 beta1 integrins interact specifically with fibronectin. Collagen type I also interacts with alpha1 beta1 and alpha2 beta1 integrins (175,177).

5.1.1. Osteoblasts

Osteoblasts can bear alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alphav, beta1, beta3 and beta5 integrin subunits depending on their stage of differentiation. Alpha2, alpha3, alpha5, alphav, beta1 and beta3 subunits are present on osteoblasts, alpha2 integrin subunit is found only on cells close to the bone surface while alphav beta3 integrins are found mainly on osteocytes (54).

Osteoblasts can adhere to 2D systems in vitro via integrin adhesion architectures that are organized as focal complexes, focal adhesions and fibrillar adhesions, but the morphology, size and subcellular distributions of these cell adhesion sites differ (178). Focal complexes that are 0.1 to 2 microns in diameter are the main precursors of focal adhesions. They recruit vinculinand phosphoproteins and exert stress between 1 and 3 nN/micron2. Focal adhesions are elongated streak-like structures (3 to 10 microns) that are often located at the cell periphery (179). They anchor bundles of actin stress fibers (F-actin) through a plaque made up of several proteins including integrins and structural proteins. Integrin-mediated adhesions called adhesomes are composed of more than 180 components (180). Adhesomes contain actin-associated proteins (tensin, vinculin, alpha actinin), adaptor proteins (vimentin, Shc-transforming protein 1) and signaling proteins (tyrosine or serine/threonine kinases and members of the Rho GTPases) (181). Three proteomic studies on the composition of adhesomes in cells attached to fibronectin and control cells on matrices like poly-L-lysine have identified more than 700 components (182184). These studies also found new focal adhesive proteins such as actin linkers (adducing) and ubiquitin ligase proteins. However, only 63 proteins associated with integrin-mediated adhesion are common to all three studies, perhaps because of the cell types and substrata used (182185). Robertson et al. recently used proteomic and phosphoproteomic methods to analyze the components of integrin-mediated adhesions that attached A375-SM human melanoma cells to polystyrene (PS) surfaces coated with fibronectin or control PS surfaces coated with transferrin (186). They found more than 1170 proteins in adhesomes, including 499 phosphoproteins. Hoffman et al. also recently used fluorescence cross-correlation spectroscopy and fluorescence recovery after photobleaching and found that some components of the adhesome existed as cytoplasmic pre-assembled complexes that were available for rapid assembly and adhesion site formation (187). Clearly, further investigations are required to give a better picture of the composition and how the adhesome is assembled.

The formation of focal adhesion sites depends on the activation state of the integrins, and this implies a change in their conformation (Figure 6). The binding of structural proteins such as talin to the cytoplasmic tail of the beta integrin subunits allows the integrin head region to be repositioned to point away from the cell surface. The cytoplasmic domain of each beta integrin subunit is therefore a major factor in establishing the connections between the cell cytoskeleton and the ECM. Integrin-linked kinases (ILK) that interact with beta1 integrin subunits are also central to integrin signaling (188). The interaction of talin with vinculin then promotes the clustering of activated integrins (189). The cytoplasmic domains of beta integrin subunits also contain one or two conserved Asn-Pro-X-Tyr, or Asn-Pro-X-Phe motifs that interact with phosphotyrosine proteins such as focal adhesion kinase (FAK) (190). FAK is a non-receptor protein tyrosine kinase that possesses three domains, an N-terminal ezrin radixin moesin (FERM) domain, a central kinase catalytic domain and a C-terminal FAT domain. FERM is involved in protein-protein interactions (191193) (for review see (194)). It interacts especially with the phenylalanine 596 in the central catalytic domain of FAK, thus inhibiting the kinase (195). The FAK kinase contains three tyrosines (Y397, Y576 and Y577); their phosphorylation is essential for the activation of FAK (196). Integrin clustering first causes the rapid autophosphorylation of FAK at Y397. FAK phosphorylated on Y397 then interacts with proteins like the Src-family kinases that phosphorylate other tyrosine residues in FAK such as the two tyrosine residues in the catalytic loop of the kinase domain (Y576 and Y577) so increasing the FAK kinase activity. These kinases also phosphorylate two proteins that interact with FAK, Crk-associated substrate (CAS) and paxillin (197). Paxillin contains many tyrosine and serine/threonine phosphorylation sites and is believed to modulate both cell adhesion and growth factor signaling pathways (198). Paxillin activates some members of the RhoGTPases family such as Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division cycle 42 (Cdc42) by recruiting Pak-interacting exchange factor and guanine exchange factor (GEF). These members of the RhoGTPases family are involved in cell adhesion and organization of the cytoskeleton (181). Their activation depends on GEF catalyzing the exchange of a GDP for a GTP on the RhoGTPases proteins. Inactivation of the RhoGTPases is mediated by a GTPase activating protein (GAP) that favors the hydrolysis of GTP to GDP. FAK-Src complex acts via p190RhoGAP to keep a RhoGTPase called RhoA inactive during the early steps of cell adhesion and spreading. In contrast, Cdc42 and Rac1 are active: Cdc42 regulates the formation of filopodia while Rac1 regulates membrane ruffling lamellipodia. RhoA becomes activated by GEFs like p115RhoGEF and p190RhoGEF at a later step in cell spreading, which favors the formation of actin stress fibers and the maturation of focal adhesions. Stimulation of RhoA suppresses the activity of Rac1 (181).

Figure 6.Activation of integrin and focal adhesion organization (E1, Epidermal Growth Factor domain 1; Beta T, beta tail) (199,200). [Illustration using Servier Medical Art, http://www.servier.fr].

Fibrillar adhesions, consisting of tensin-alpha5 beta1 integrin complexes that bind to fibronectin, are more centrally located in the cell than are focal adhesions. The formation of fibrillar adhesions is closely linked to the capacity of cells to polymerize fibronectin to form ECM fibrils (201,202). The translocation of fibrillar adhesions is highly directional, proceeding centripetally from the cell periphery towards the center and is always aligned along the long axis of the focal adhesion. Multi-ligand alphav beta3 integrins remain within focal adhesions, while alpha5 beta1 integrins are translocated at 6.5 microns/h parallel to the actin microfilaments in fibrillar adhesions (201,202). Lin et al. recently reported that osteoblasts at 12 hours organize endogenous fibronectin into fibrils on self-assembled monolayers bearing amine groups, while those bearing hydroxyl and methyl groups limit such fibronectin fibril formation (203).

However, it is known that the components of 2D and 3D adhesions differ considerably: 3D adhesions used mainly alpha5 beta1 integrins instead of alphav beta3 and had a low FAK phosphorylation (204).

5.1.2. Osteoclasts

Osteoclasts contain alpha2, alpha5, alphav, beta1 and beta3 integrin subunits. However, the adhesion receptor alphav beta3 is the major integrin expressed by osteoclasts and a marker of the osteoclast phenotype. The engagement of alphav beta3 integrin leads to the formation of a multi-molecular complex that includes c-Src and PI3K (205). In addition to its role in cell-matrix attachment, alphav beta3 is also involved in other aspects of osteoclast biology, and osteoclasts that lack alphav beta3 are dysfunctional (206,207). The bone matrix mediates anti-apoptotic signals via integrins, and alphav beta3 occupancy promotes osteoclast survival (208). In contrast, unoccupied alphav beta3 sites may induce osteoclast apoptosis via caspase-8 activation, and in the absence of alphav beta3 (beta3-/- knockout mice), delayed cell death occurs as a result of caspase-9 activation (209,210).

Upon adhesion, osteoclasts reorganize their cytoskeleton and form podosomes which are small adhesion structures (1 micron diameter) with a dense actin core surrounded by a rosette-like structure containing proteins such as alphav beta3 integrins, structural focal adhesive proteins (talin and vinculin), actin-associated proteins (gelsolin and alpha-actinin), tyrosine kinases (c-Src and Pyk2) and small GTPases. RhoGTPases have been involved in the organization of F-actin cytoskeleton in different cell types, and in mature osteoclasts Rac, Cdc42 and Rho regulate the podosome formation into the sealing zone (211213). The podosome core also contains large amounts of proteins involved in actin polymerization. Thus, the Arp2/3 complex that is activated by Wiscott-Aldrich syndrome protein (WASP) takes part in the formation of the actin podosome core, while the formin protein initiates the unbranched F-actin strands and the formation of stress fibers (214). Cortactin, which interacts with the Arp2/3 complex, is also involved in the formation and stabilization of the branched actin network (215). By contrast, formation of the ruffled membrane involves vesicular trafficking regulated by the Rab family of GTPases, particularly lysosomal Rab7 (216).

Osteoclasts are capable of adhesion on different substrates by distinct F-actin structures. When osteoclasts adhere on plastic or glass, podosomes form clusters organized in a podosome belt around the cell, in which podosomes can be individualized (Figure 7). Upon adhesion to bone or mineralized ECM, podosomes undergo major reorganization as part of the osteoclastic terminal differentiation into bone resorbing cells. In non-active (non-resorbing) unpolarized osteoclasts, podosomes are dispersed on the adhesion surface or organized in a loose belt of podosomes (216). When osteoclasts are activated to resorb bone, they strongly attach to the bone matrix by densely packed F-actin rich podosomes to form the sealing zone that delineates the cellular area in contact with the bone surface where the ruffled membrane enlarges and beneath which the resorption lacuna occurs (42,217). Some studies suggest that a podosome belt prefigures the sealing zone but this is rather controversial (217220). Microtubules, made up of alpha-and beta-tubulin heterodimers in filamentous networks, also regulate the podosome organization at the end of osteoclast maturation (217). Podosomes are also involved in the migration of osteoclasts, perhaps through a saltatory mode (221). Touaitahuata et al. suggested recently that adhesion is essential for osteoclast differentiation while podosome formation is not (219).

Figure 7. Bone resorption and actin ring formation. Mature osteoclasts were obtained from cord blood monocytes in long-term cultures in presence of MCSF and RANKL. The cells were allowed to settle either on devitalized bovine bone slices or plastic. (A) Bone resorption appears as dark areas after toluidine blue staining, with a bright aspect under epi-illumination. (B) Cell cultured on plastic and F-actin, found in actin ring, was stained with fluorescent-labeled phalloidin (red) and nuclei with DAPI (blue).

5.2. Influence of ECM-integrin interactions on cell behavior

Integrins that are involved in the interactions between cells and the ECM may also influence the responses of cells to growth factors such as BMPs, VEGFs, PDGFs and IGFs (222225). Integrins and growth factors influence each other in many ways. Integrins can be co-localized with growth factor receptors. Lai and Chen demonstrated that specific integrin subunits (alphav, beta1, beta3, alphav beta3, alphav beta5, alphav beta8) were located with type I and type II (BMPRI and BMPRII) BMP receptors (222). They also showed that blocking the alphav integrin subunits with antibodies decreased the ALP activity induced by BMP-2. Goel et al. found that IGFIR was located together with beta1A integrins at focal adhesion points in GD25 cells (224). Interactions that involve the insulin receptor substrate-1 (IRS-1) maintain IGFIR in an activated state that favors cell proliferation and tumor growth. In contrast, beta1C integrin subunits that form a complex with Gab1/Shp2 promote the recruitment of Shp2 to IGFIR, which then leads to dephosphorylation of the receptor. This inactivation of IGFIR slows down cell proliferation and thus inhibits tumor growth (224).

Integrin also act on growth factor signal transduction by recruiting adaptors to the plasma membrane or specific signaling proteins involved in the adhesome. Integrins can induce the phosphorylation of growth factor receptors in the absence of their ligand (226). Veevers-Lowe et al. found that the PDGF receptor beta in human bone marrow MSCs attached to fibronectin via their alpha5 beta1 integrins was already phosphorylated, without any growth factor stimulation (226). This activation is essential for MSC migration (226). The N-terminal FERM domain of FAK also interacts with growth factor receptors such as those of PDGF (226229). In addition, Tamura et al. demonstrated that FAK was essential for the differentiation of MC3T3-E1 preosteoblasts induced by recombinant human BMP-2 (230). No ALP activity was detected in FAK-deficient cells treated with BMP-2.

These versatile integrins also modulate the internalization and degradation rate of growth factor receptors. Reynolds et al. showed that the alphav beta3 inhibitor stimulated tumor growth and tumor angiogenesis by recycling the VEGFR2 internalized in endosomes back to the plasma membrane and by decreasing VEGFR2 degradation (223). Finally, integrins stimulate the synthesis of growth factors. Mai et al. reported recently that silencing the gene encoding for the beta1 integrin subunit inhibited the synthesis of BMP-2 and the differentiation of MC3T3-E1 preosteoblasts into osteoblasts by preventing activation of the gene encoding ALP (231).

However, growth factors also regulate the synthesis of specific integrins. Mena et al. observed that stimulating human umbilical vein endothelial cells (HUVECs) with VEGF-A increased the production of alpha5 and beta1 integrin subunits (232). Similarly, our research group has shown that a peptide derived from the knuckle epitope of BMP-9 increased the amount of alphav integrin subunits in the plasma membrane of MC3T3-E1 preosteoblasts attached to PS coated with peptides derived from BSP (233). Lai and Chen also showed that stimulating human osteoblasts (HOB19) with BMP-2 led to the synthesis of alphav, beta1 and beta3 integrin subunits in human osteoblasts (222). As BMP-2 increased the amount of alphav beta3 integrins in human osteoblasts, it favored their adhesion to OPN and vitronectin (222).

The crosstalk between integrin and growth factor signaling pathways coordinates MSCs and bone cells behaviors. Researchers that develop biomimetic materials must therefore be aware of such complex interactions. They have to get a better understanding of the influence of biomimetic materials on the ability of cells to respond to growth factors, cytokines and hormones.

6. Biomimetic materials

6.1. Materials functionalized with proteins

The biomaterials used in bone repair can be inorganic materials, natural polymers, synthetic polymers, or even composites. But not all bone biomaterials currently in use are osteoconductive and osteoinductive (for review see (8,234)). Biomaterials can be functionalized with ECM proteins such as fibronectin, collagen, OPN and BSP to increase their interactions with cells (53,235). The proteins can be immobilized on the surfaces by non-specific adsorption (physisorption) or by covalent binding (for review see (8)). Schwab et al. tested fibronectin, vitronectin and OPN adsorbed onto PS. Fibronectin and OPN promoted greater hMSC proliferation in comparison with the control after 5 and 10 days in standard growth medium (53). In addition, MSCs attached to fibronectin in osteogenic medium contained more BSP and Runx2 after 5 days, while MSCs attached to OPN contained these markers only after 10 days in culture. Cells on vitronectin contained slightly more osteogenic markers, Runx2 and BSP, after 10 days in osteogenic medium than did cells in standard growth medium. Fibronectin and OPN induced MSCs in osteogenic medium to deposit more calcium than did cells on vitronectin (53).

Several studies have shown that the density and conformation of the proteins adsorbed or grafted onto the surface can strongly affect cell behavior (236,237). Faia-Torres et al. used a gradient of fibronectin (48-213ng/cm2) on PCL surfaces and showed that human bone marrow MSCs incubated with intermediate densities of fibronectin for 7 days proliferated most rapidly. On the other hand, cells incubated for 7 and 14 days in lower and higher densities of fibronectin had more ALP activity and collagen type I synthesis than did hMSCs on intermediate fibronectin densities (237). Lin et al. also used self-assembled monolayers with terminal −OH, −CH3, and −NH2 groups to evaluate the conformation of adsorbed fibronectin (203). They showed that fibronectin adsorbed onto self-assembled monolayers with terminal -OH groups had more accessible cell-binding domains than did the fibronectin on surfaces with terminal -CH3 and-NH2 groups. The more accessible cell binding domains of fibronectin adsorbed onto surfaces with terminal-OH groups led to greater amount of vinculin and tensin and favored initial cell adhesion at 2h as shown by an increased area of focal and fibrillar adhesions in primary osteoblasts from rat calvaria (203).

The use of proteins extracted from the ECM presents other challenges. There can be batch-to-batch variations (238). ECM proteins which are extracted and purified from non-human species can increase the risk of undesirable immune responses and infections (238). Peptides derived from proteins that contain the sequence recognized by specific cell membrane receptors have therefore been developed (Table 1). A major advantage of these peptides is that they are readily synthesized and purified, which reduces their production costs (239).

Table 1. Effect of ECM-derived peptides on the behavior of bone cells
ProteinPeptide sequenceNegative controlCulture systemEffectReferences
LamininCRARKQAASIKVAVSADR (IKVAV)In vitro (Adwipose derived stem cells(AdSC) from human tissue)-↑ Number of AdSC onto PCL-IKVAV after 24h and 48h compared with PCL alone(256)
DLTIDDSYWYRI(alpha2 chain)Scramble peptideIn vitro (Human osteosarcoma cells)-↑ Number of cells on Ti-coated with the peptide after 1 day compared with Ti alone
-↑ ALP activity of cells onto Ti-coated with the peptide after 1 day compared with Ti alone. No difference after 3 days (Ti, Ti-coated with positive or negative peptide)
(257)
DLTIDDSYWYRI(alpha2 chain)Scramble peptideIn vitro (MG63 osteoblast like cells)-↑ number and area of cells on PS coated plates with laminin/PS coated with the peptide compared with PS coated with BSA/PS coated with a scramble peptide after 1h in serum-free medium
-↑ osteogenic markers for differentiation(BSP, OC) of cells on Ti disk coated with the peptide after 7 days compared with Ti alone
-↑ ALP activity of cells on Ti disks coated with the peptide after 3 days compared with Ti alone
-↑ number of cells on Ti disks coated with the peptide after 1h compared with Ti alone
(258)
Scramble peptideIn vivo (NewZealand white rabbits)-↑ bone area for Ti implant coated with the peptide after 1 week compared with Ti alone. No difference after 2 and 4 weeks (Ti, Ticoated with positive or negative peptide)
-↑ collagen deposition for Ti implant coated with the peptide after 1 week compared with Ti alone. No difference after 4 weeks (Ti,Ticoated with positive or negative peptide)
-↑ ALP activity for Ti implant coated with the peptide after 4 weeks compared with Ti alone or Ti with negative peptide
BSPCGGNGEPRGDTYRAYCGGNGEPRGETYRAYIn vitro (MC3T3-E1)-Cytoskeletal organization of cells on PCL films functionalized with the peptide after 1h compared with PCL alone or with negative peptide in serum-free medium
-↑ area of cells on PCL films functionalized with the peptide after 1h compared with PCL alone or with negative peptide in serum-free medium
(240)
NGVFKYRPRYYLYKHAYFYPHLKRFPVQIn vitro (Human muscle-derived stem cells)-↑ ALP activity and calcium deposits in a dose-dependent manner of peptide derived from BSP after 14 days in osteogenic medium
-↑ mRNAs of osteogenic genes(ALP, type I collagen, OC, Run×2) was a dose-dependent manner of peptide derived from BSP after 14 days in osteogenic medium
(259)
FibronectinRGD-PHSRN K3G4RGDS and K3G4PHSRNIn vitro (MC3T3-E1)-↑ number of cells on PHSRN-RGD Ti surface after 4h compared with RGD or PHSRN Ti surfaces alone -No difference in term of cellular differentiation(ALP activity) after 14 days(260)
Collagen type IGGGGDGEASPIn vitro (Primary rat MSCs)-↑ ALP activity of cells trapped in hydrogel modified with DGEA peptide after 7 days compared with unmodified hydrogel
-↑ number of cells in hydrogel modified with DGEA after 30 days compared with hydrogel modified with RGD or a mixture of RGD and DGEA
-↑ collagen type I in cells trapped in hydrogel modified with DGEA after 30 days compared with hydrogel modified with a mixture of RGD and DGEA
-↑ OC production and mineralization in cells trapped in hydrogel modified with DGEA after 30 days compared with unmodified hydrogel or hydrogel modified with RGD or a mixture of RGD and DGEA
(242)
GGYGGGPC(GPP) 5GFOGER (GPP) 5GPCIn vitro (hMSCs)-↑ migration of cells on GFOGER-coated random PCL nanofiber after 48h compared with uncoated or collagen-coated meshes. No difference between GFOGER-and collagen-coated aligned meshes
-↑ ALP activity of cells on GFOGER-coated random meshes after 21 days compared with uncoated or collagen-coated meshes. ↑ ALP activity of cells on GFOGER-coated aligned meshes compared with collagen-coated meshes
-↑ calcium w by cells on GFOGER-coated random meshes after 21 days compared with uncoated or collagen-coated meshes. Nodifference between GFOGER-and collagen-coated aligned meshes
(243)
RGDIn vitro (MC3T3-E1)-↑ ALP activity and calcification on Ti-RGD after 28 days compared with Ti alone in differentiation-inducing medium(261)
In vitro (hMSCs)-↑ actin cytoskeletal organization and paxillin at focal adhesions in cells onto PLLA/BCP scaffold modified with RGD after 24h compared with unmodified scaffold
-↑ number of cells and their spreading on PLLA/BCP scaffold modified with RGD after 24h compared with unmodified scaffold
-↑ ALP activity after 7 days and mineralization after 14 days in cells onto PLLA/BCP scaffold modified with RGD compared with unmodified scaffold
(241)
KRSRGYCKSRRGYCIn vitro (MC3T3-E1)-↑ focal adhesions, actin fibers and number of cells on KRSR-TiO2 anodized nanotubes compared with KSRR-TiO2 anodized nanotubes or nanotubes alone after 4h
-↑ ALP activity and OC, BSP, OPN, Run×2, Osx and ALP mRNAs for cells on KRSR-TiO2 after 7 and 14 days compared with KSRR-TiO2 anodized nanotubes or nanotubes alone
(246)
KRSRCRKSRCIn vitro (Primary human osteoblast, NHOsts)-↑ number of cells onto calcium aluminate functionalized with KRSRC compared with calcium aluminate alone or with the negative peptide, from 1 day to 7 days -Spread and elongated cells on calcium aluminate functionalized with KRSRC after 4 days compared with calcium aluminate alone where cells were rounded(262)
GGGGGGGKRSRIn vitro (hMSCs)-↑ number of cells on HAP coated with KRSR after 1h compared to HAP alone -Combination with RGD peptide had no effect on the number of cells -Cells slightly spread on HAP coated with KRSR or in combination with RGD after 1h compared with HAP alone where cells were rounded(263)
GGGGGGGFHRRIKAIn vitro (hMSCs)-↑ number of cells on HAP coated with FHRRIKA after 1h compared with HAP alone
-Combination with RGD peptide had no effect on the number of cells
-Cells slightly spread on HAP coated with FHRRIKA or combined with RGD after 1h compared with HAP alone where cells were rounded
(263)
AdSC: Adipose derived stem cells, BSA: Bovine serum albumin

6.2. Homogeneous peptide-modified surfaces

One of the most frequent sequences in many proteins is the RGD peptide. A RGD sequence derived from the BSP (CGGNGEPRGDTYRAY, pRGD) grafted to PCL films induced the formation of focal adhesions and organization of the actin cytoskeleton in MC3T3-E1 preosteoblasts placed in serum-free medium for 1h. The cells also had a greater cell surface area than those on the negative peptide CGGNGEPRGETYRAY (240). More importantly, only the MC3T3-E1 preosteoblasts attached to PCL functionalized by pRGD responded to BMP-2 (100 ng/mL). Shin et al. also used a composite scaffold of poly (L-lactide) (PLLA) and biphasic calcium phosphate (BCP) grafted with an RGD peptide to study the impact of adhesive peptides on the behavior of hMSCs (241). The cells on the composite scaffold modified with the RGD peptide for 24h contained actin stress fibers and paxillin at the focal adhesions while those on the unmodified scaffold had none. There were also more spread cells on the RGD-modified scaffold. The hMSCs on the RGD-modified scaffold for 7 days had more ALP activity and, after 14 days, greater mineralization than did cells on the unmodified scaffold or PLLA grafted with RGD (241). Since type I collagen is the major component of bone osteoid, several peptides derived from this protein such as DGEA and GFOGER have been developed (242244). The research group of Garcia has shown that GFOGER-coated PCL scaffolds significantly improved the repair of femoral bone defects in rats, 12 weeks after implantation (244). Furthermore, Mehta et al. showed that the ALP activity of rat MSCs trapped in an alginate hydrogel containing a DGEA peptide (H2N-GGGGDGEASP-OH) for 7 days was greater in comparison with the controls (alginate alone or in combination with RGD) (242). Their production of OC and their mineralization were also increased (242).

Other peptides that could improve the adhesion, proliferation and differentiation of bone cells are those containing a heparin-binding site that interacts with heparan sulphate proteoglycans at the cell surface (245,246). One peptide sequence that binds to heparan sulphate proteoglycans is Lys–Arg–Ser–Arg (KRSR), which is found in proteins such as fibronectin, vitronectin and BSP (247). Sun et al. have shown that MC3T3-E1 preosteoblasts on titanium dioxide (TiO2) anodized nanotubes functionalized with KRSR had more vinculin at focal points and a better organized actin cytoskeleton than did those on nanotubes functionalized with the negative peptide KSRR or the control nanotubes (246). The osteogenic differentiation of MC3T3-E1 preosteoblasts attached to TiO2 anodized nanotubes functionalized with KRSR was also better, as shown by the increased concentrations of mRNA encoding osteogenic markers (ALP, BSP, Runx2, OPN, Osx and OC) and ALP activity after 7 and 14 days.

There are many other challenges in the use of peptides-functionalized surfaces, such as the way they are immobilized on the surface to retain their bioactivity, their density and conformation (248). Rezania and Healy compared the effect of peptides derived from BSP (CGGNGEPRGDTYRAY) immobilized on a quartz surface at densities from 0.01 to 3.8 pmol/cm2 on the osteoblastic differentiation of rat calvaria osteoblast-like cells (249). A density of at least 0.62 pmol/cm2 of BSP peptides was necessary to increase the mineralization in rat calvaria osteoblast-like cells.

The conformation of the grafted peptide also influences cell behavior. The RGD peptide can be used in a linear or cyclic conformation. Cyclic RGD peptide is more stable than the linear peptide at physiological pH (250). Kilian et al. also showed that the cyclic RGD (RGDfC where f is a phenylalanine residue with a D configuration) enhanced focal adhesion and increased MSC spreading more than did the linear RGD (GRGDSC) (251). The cyclic RGD peptide could also enhance the affinity of cells for the alphav beta3 integrin better than did the linear one (252,253).

Another important challenge is the sterilization of biomimetic materials that use bioactive molecules. The main sterilization methods used are moist heat, dry heat, gamma radiation, ultraviolet radiation, hydrogen peroxide, and ethylene oxide (254,255). RGD peptide dissolved in ultrapure water is completely broken down after exposure to UVC for 12h, while ethylene oxide sterilization alters proteins by forming adducts between the ethylene oxide and the protein methionine and cysteine residues (254,255).

6.3. Mixed peptide surfaces

6.3.1. Combinations of adhesive peptides

It is possible to immobilize a combination of peptides derived from different ECM proteins on the biomaterial surface so as to mimic more precisely bone physiology and so improve the interaction between the material and the cells. The frequently used RGDS peptides have been immobilized together with KRSR peptides on silk fibroid nanofibers (264). This combination increases the proliferation of human osteoblasts over that of cells on nanofibers of silk fibroid alone. However, the potential of RGD and KRSR peptides combinations for bone application may be controversial. A combination of GCRGYGRGDSPG and GCRGYGKRSRG peptides on a titanium implant has no greater synergistic effect on bone formation in maxillae of miniature pigs than does KRSR alone (265).

The two immobilized peptides can also be derived from the same protein. Fibronectin possesses a synergistic site PHSRN in its FNIII9 domain that enhances the interaction between the RGD sequence in its FNIII10 domain and alpha5 beta1 integrin (266). Chen et al. developed a simple technique for immobilizing the bioactive fibronectin motifs, RGD (KKKGGGGRGDS) and PHSRN (KKKGGGGPHSRN) on Ti surfaces modified with (3-chloropropyl) triethoxysilane (CPTES) (260). This combination of RGD and PHSRN peptides enhanced the adhesion of MC3T3-E1 preosteoblasts more than did either of the peptides alone, but there were no difference in the osteoblastic differentiation after 14 days (260). The best proportion between the two peptides that favors the desired cell response and tissue repair must be determined. Nakaoka et al. used alginate gels functionalized with different ratios of RGD (GGGGRGDSP) and PHSRN (GGGGPHSRN) peptides to show that cells seeded on alginate containing 67 percent RGD and 33 percent PHSRN peptides had more OC than did cells on gels containing 11 percent RGD and 89 percent PHSRN (267). The two bioactive RGD and PHSRN motifs can be combined to create a longer peptide and so overcome the peptide ratio problem. Benoit and Anseth developed a peptide containing the RGD and PHSRN motifs separated by 13 glycine residues (RGDG13PHSRN) (268). This peptide grafted on a poly (ethylene) glycol (PEG) hydrogel favored the organization of the actin cytoskeleton in osteoblasts from neonatal rat calvaria more than did ungrafted PEG.

Peptides derived from the adhesive protein fibronectin can be immobilized with peptides extracted from type I collagen so as to better mimic the properties of the bone matrix. Reyes et al. immobilized the peptide GFOGER (GGYGGGPC(GPP)5GFOGER(GPP)5GPC) that targets the integrin alpha2 beta1 with a fragment of fibronectin containing the sequences RGD and PHSRN recognized by alpha5 beta1 integrins (269,270). This strategy activates specific integrins and improves both the adhesion of human fibrosarcoma cells and the activation of their FAK more than any of the peptides alone. Visser et al. designed a peptide containing the decapeptide collagen-binding (CBD) motif derived from the von Willebrand factor with an RGD motif at the C-terminus (WREPSFMALSGRGDS) (271). Absorbable collagen type I sponges (ACSs) functionalized with CBD-RGD increased the ALP activity of rat spinal bone marrow MSCs by day 10 and enhanced mineralization of the matrix on day 21. Moreover, in vivo experiments in rats showed that with the injection of ACS functionalized by CBD-RGD in combination with BMP-2 formed ectopic bone after 21 days compared with ACSs containing BMP-2 alone (271).

These strategies can also be used to improve growth factor efficiency. The CBD motif has been used to develop chimeric recombinant proteins such as CBD-TGF-beta fusion proteins that enhance the delivery of the growth factor to the cells (272).

6.3.2. Mixed adhesive peptides with BMPs or their derived peptides

Adhesive peptides can be used in combination with growth factors such as the BMPs to develop synthetic osteoinductive materials (Table 2). Shekaran et al. designed a PEG gel functionalized with the GFOGER peptides (GGYGGGP(GPP)5GFOGER(GPP)5GPC) using maleimide chemistry in combination with embeded BMP-2 (0.03, 0.06 and 0.3 microg per 1.5 microL of hydrogel) (273). Implanting these hydrogels in defects in the radius bone of B6129SF2/J wild type male mice resulted in better bone reconstruction in the presence of BMP-2 (mineral density and bone volume) than did implants of hydrogel without BMP-2.

Table 2. Targeting specific integrins to improve cell response to growth factors
IntegrinsGrowth factorCulture systemBehaviorReferences
Beta1BMP-2In vitro (MC3T3-E1)-Blocking the beta1 integrin subunit↓the BMP-2 transcription and secretion induced by mechanical stimulation
-BMP-2 induces MC3T3-E1 differentiation(↑ in Run×2, Osx and ALP mRNA at 12h)
(231)
BMP-2In vitro (MSCs)-↑ beta1 synthesis on alumina surface functionalized with BMP-2 after 2 days compared with alumina alone(284)
Alpha2 Beta1BMP-2In vivo (B6129SF2/J wild-type mice)-Scaffold targeting alpha2 beta1 integrin loaded with BMP-2 permitted better bone healing than scaffold targeting the integrin alone(273)
BMP-2, BMP-7In vitro (hMSCs from healthy and osteoporotic patient)↓ in alpha2 integrin subunit was concomitant with a↓in cell migration induced by the BMPs compared with control(285)
Alpha5 Beta1BMP-2In vitro and In vivo (MSCs, Sprague-Dawley rats)Fibronectin derived fragments:
 -↑ cell adhesion over hyaluronic acid hydrogel alone
 -↑ bone formation invivo than hyaluronic acid hydrogel alone
(286)
VEGF-A (20 ng/mL)In vitro (HUVEC)-↑ in amounts of alpha5 and beta1 integrin subunits(2-4h)
-↑ in COX-2 mRNA induced by VEGF-A
(232)
IGF-2In vitro (hMSCs)-Silencing alpha5 and beta1 integrin subunits ↓ IGF-2 synthesis
-Alpha5 beta1↑IGF-2 signaling via FAK and PI3K pathways
(287)
Alphav Beta3VEGFIn vitro (HUVEC)-↑ beta3 integrin phosphorylation in the presence of VEGF
-Used antibodies blocking alphav and beta3 integrin subunits to inhibit VEGFR-2 phosphorylation
(288)
FGF-1In vitro (NIH3T3)-FGF-1 bound directly to alphav beta3(289)
FGF-1In vitro (NIH3T3, HUVEC)-FGF-1 formed ternary complex with the integrin alphav beta3 and the FGFR needed to maintain Erk1/2 phosphorylation(290)
IGF-1 (200 ng/mL)In vitro (OSE-3T3)-IGF-1 promoted Cbfa1 activity using the PI3K pathway
-Used an inhibitor of alphav beta3 occupancy to inhibit Cbfa1 activity induced by IGF-1
(291)
BMP-2In vitro (MC3T3-E1)-rCYR61 influenced the differentiation of MC3T3-E1 preosteoblasts by BMP-2 via the alphav beta3 integrin
-↑ in rCYR61 induced an↑in BMP-2 synthesis
(292)
pBMP-9 (400 ng/mL)In vitro (MC3T3-E1)-pBMP-9 induced↑in alphav integrin subunit in cell membrane
-pBMP-9 induced more↑in ALP activity after 24h than in unstimulated cells
(233)
RGD+pBMP-2In vitro (hMSCs)-↑ mineralization of hBMSCs after 14 days more than pBMP-2 or RGD alone(279)
pBMP-2: peptide-derived from BMP-2

Both adhesive peptides and BMPs can be covalently bound to surfaces or scaffolds. He et al. functionalized a poly(lactide-ethylene oxide fumarate) (PLEOF) hydrogel with GRGD peptides, one peptide derived from residues 162−168 of OPN and another derived from residues 73−92 of BMP-2 (24). Each peptide was covalently bound to the PLEOF polymer using three types of chemistry. The GRGD peptide contained an acrylamide function which bound to the crosslinker N,N-methylenebis(acrylamide) during the formation of the covalent gel, the OPN used an oxime reaction involving the –O-NH2 function on the OPN peptide with the aldehyde group from the hydrogel, while the BMP-2-azide derived peptide was attached via a click chemistry involving the propargyl function of the hydrogel. He et al. used bone marrow MSCs isolated from young adult Wistar rats to show a greater increase in the ALP activity (14 days) and calcium content (28 days) in MSCs on this hydrogel than in cells seeded on hydrogels containing RGD alone or RGD plus BMP-2 (24). The combination of the 3 peptides (RGD, BMP-2 and OPN) also improved the production of vasculogenic markers like PECAM-1 and VE-cadherin in vitro (24). Osteogenic and vasculogenic differentiation could greatly enhance the process of bone repair, as good vascularization of the biomaterial delivers more oxygen and the nutrients necessary for the proper functioning of bone cells. However, the size of the protein (for example BMP) used in combination with short adhesive peptides can block the interaction between the cells and the short peptides.

BMPs can be replaced by short derived peptides to overcome this problem. These peptides can mimic the knuckle epitope of the BMPs and interact with type II receptors such as BMPRII (274). Our research group has developed a peptide derived from BMP-9 (pBMP-9) based on the studies of Suzuki et al. and Saito et al. on peptides derived from the knuckle epitope of BMP-2. We have shown that pBMP-9 (Ac-CGGKVGKACCVPTKLSPISVLYK-NH2) promoted the differentiation of murine MC3T3-E1 preosteoblasts in the same way as the entire protein BMP-9 (275277). It also induced woven bone formation when injected, together with chitosan, into the quadriceps of mice (278). However, the early differentiation of murine MC3T3-E1 preosteoblasts seems to depend on the type of integrins involved in cell adhesion. Marquis et al. found that murine MC3T3-E1 preosteoblasts in the presence of pBMP-9 had enhanced ALP activity when they were adhered to polystyrene coated with BSP-derived peptides, while cells on peptides DGEA-coated polystyrene targeting alpha2 beta1 integrins showed no such increase (233).

Selection of the adhesive peptides to combine with peptides derived from BMPs is therefore critical. He et al. immobilized the adhesive peptides GRGD (1.62 pmol/cm2) and peptide P4 derived from BMP-2 (KIPKASSVPTELSAISTLYL) (5.2 pmol/cm2) on PLEOF hydrogels (27). This combination acted synergistically on the commitment of rat MSCs to osteoblast lineage by increasing the ALP activity and stimulating better mineralization of the matrix than did peptides grafted alone (27). These same two peptides have been used to functionalize self-assembled monolayers using click chemistry, to create a concentration gradient (279). The combination of equal amount of GRGDS and P4 (65 pmol/cm2 of each peptide) synergistically up-regulated the number of BSP transcripts in human bone marrow stromal cells and mineralization of their matrix without any osteogenic supplements (279). Peptides derived from the knuckle epitope of BMP-2 (KIPKACCVPTELSAISMLYL), BMP-7 (TVPKPSSAPTQLNAISTLYF) and BMP-9 (KVGKASSVPTKLSPISILYK) and adhesive peptide (GRGDSPC) have been grafted onto the surface of polyethylene terephthalate (PET) to investigate the differentiation of mouse MC3T3-E1 preosteoblasts (280). These modified PET increased the cell contents of Runx2 transcripts and the production of ECM. A recent study that evaluated the synergistic effect of a peptide derived from BMP-7 (GQGFSYPYKAVFSTQ) and a cyclic RGD peptide grafted to a quartz substrate on the behavior of MSCs found that these two peptides increased the ALP activity and the matrix mineralization (281).

Another recently developed strategy uses self-assembled peptides that mimic the matrix (for review see (282)). These peptides can have a beta sheet motif and even a coiled-coil motif to assemble alpha helices into ordered structures. The osteoblastic differentiation of C2C12 cells can be increased by peptides amphiphiles TSPHVPYGGGS that bind with high affinity to BMP-2 (283).

We therefore should investigate combinations of several BMPs and growth factors with adhesive peptides so as to develop osteoinductive biomimetic materials that act in synergy to favor bone healing. This will be challenging because growth factors can trigger antagonistic intracellular signaling (8). BMP-2, BMP-7 and BMP-9 are not members of the same BMP subfamily and they interact with different type I receptors. They therefore activate different non-canonical pathways. Our research group has recently demonstrated that BMP-2 and BMP-9 did not act synergistically when used to treat MC3T3-E1 preosteoblasts seeded on PCL films functionalized with a peptide derived from BSP due to different level of activation of the canonical Wnt pathway (21).

7. Conclusion

Bone healing process involves complex interactions between several cell types and signaling molecules. Autografts, the current gold standard for repairing bone defects, have several limitations. Therefore, biomaterials functionalized with adhesive peptides that favor bone cell attachment have been combined with growth factors, especially BMPs and their derived peptides, in order to optimize bone healing. However, it is essential to understand the crosstalk between the integrins, which interact with these adhesive peptides, and growth factors so as to understand the signaling that will direct cell behavior such as the ability of stem cells to differentiate into mature osteoblasts. While it is true that using combinations of adhesive peptides and BMPs or their derived peptides to create biomimetic materials has given promising results, challenges remain, especially questions of peptide density, conformation, graft stability and sterilization methods.

8. Perspectives

We still face numerous challenges as we strive to create osteoinductive biomimetic materials in 3D using bioactive molecules since most current studies are performed in 2D cultures. Moreover, most studies have focused on the impact of BMPs on bone cell responses despite the fact that many other growth factors, such as FGF and VEGF, are involved in bone healing. We need a combination of several growth factors that together optimize the action of bone substitutes. Finding such a combination will not be easy, but will promise great benefits for the patients.

9. Acknowledgements

We thank Dr Owen Parkes for proofreading the manuscript. This research was supported through a Research Chair of Canada (Laboratory of Cells-Biomaterials Biohybrid Systems) and grant from the Natural Sciences and Engineering Research Council of Canada.

Abbreviations: ACS: Absorbable collagen type I sponge, AdBMP: adenovirus encoding BMP, AdSC: adipose derived stem cell, ALP: alkaline phosphatase, AP-1: activating protein-1, APC: adenomatous polyposis coli, ASARM: acidic serine-rich and aspartate-rich motif, BAMBI: BMP and activin membrane-bound inhibitor, BCP: biphasic calcium phosphate, beta T: beta tail, BMP: bone morphogenetic protein, BMPR: BMP receptor, BSA: bovine serum albumin, BSP: bone sialoprotein, CAS: Crk-associated substrate, CBD: decapeptide collagen-binding, Cdc42: cell division cycle 42, CPTES: (3-chloropropyl) triethoxysilane, CSL: CBF1, Suppressor of Hairless, Lag-1, DAG: diacylglycerol, DAP12:12 kDa DNAX-activating protein, DC-STAMP: dendritic cell-specific transmembrane protein, Dkk-1: Dickkopf-1, Dvl: Dishevelled, E1: epidermal Growth Factor domain 1, ECM: extracellular matrix, EGF: epidermal growth factor, EphB4: Ephrin type-B receptor 4, ERK1/2: extracellular signal regulated kinase 1/2, F-actin: filamentous actin (stress fiber), FAK: focal adhesion kinase, FERM: N-terminal ezrin radixin moesin, FGF: fibroblast growth factor, FGFR: FGF receptor, Fzd: Frizzled, GAP: GTPase activating protein, GEF: guanine exchange factor, GFs: growth factors, Grb2: growth factor receptor-bound protein 2, GSK-3 beta: glycogen synthase kinase-3 beta, HAP: hydroxyapatite, Hes: Hairy enhancer of split, Hey: Hes-related with the YRPW motif, Hh: Hedgehog, hMSC: human MSC, IFN gamma: interferon-gamma, IGF: insulin-like growth factor, IGFIR: IGF type I receptor, IL: interleukin, ILK: Integrin-linked kinase, IP3: inositol-1,4,5-trisphosphate, IRS-1: insulin receptor substrate-1, I-Smad: inhibitory Smad, JNK: c-jun N-terminal kinase, KRSR: Lys–Arg–Ser–Arg, LRP5/6: low density lipoprotein receptor related protein 5 and 6, MAML: Mastermind-like, MAPK: mitogen activated protein kinase, M-CSF: macrophage-colony stimulating factor, MEPE: matrix extracellular phosphoglycoprotein, MMP: matrix metalloproteinases, MSC: mesenchymal stem cell, NFATc1: nuclear factor of activated T cells cytoplasmic 1, NICD: Notch intracellular domain, OC: osteocalcin, OPG: osteoprotegerin, OPN: osteopontin, OSCAR: osteoclast-associated receptors, Osx: osterix, pBMP-2: peptide derived from BMP-2, pBMP-9: peptide derived from BMP-9, PCL: polycaprolactone, PDGF: platelet-derived growth factor, PEG: poly (ethylene) glycol, PET: polyethylene terephthalate, PI3K: phosphatidyl inositol 3 kinase, PLCgamma: phospholipase C gamma, PLEOF: poly(lactide-ethylene oxide fumarate), PLLA: poly (L-lactide), PPM1A: protein phosphatase, Mg2+/Mn2+ dependant, 1A, PS: polystyrene, PTH: parathyroid hormone, Rac1: Ras-related C3 botulinum toxin substrate 1, RANKL: receptor activator of NF-kappaB ligand, RBP-J: recombinant recognition sequence binding protein at the Jkappa site, RGD: Arg-Gly-Asp, Sema4D: semaphorin 4D, Shc: Src homology 2 domain-containing, SIBLING: small integrin-binding ligand N-linked glycoproteins, siRNA: small interfering RNA, SLRP: small leucine-rich proteoglycans, Sos: son of sevenless, SP-1: sphingosine-1 phosphate, STKR: Ser/Thr kinase receptor, TAK1: TGF-beta activated tyrosine kinase 1, TCF/LEF: T-cell factor/lymphoid enhancer factor, TGF-beta: transforming growth factor-beta, Ti: titanium, TiO2: titanium dioxide, TNF: tumor necrosis factor, TRAF6: TNF receptor associated factor-6, TRAP: tartrate resistant acid phosphatase, TREM-2: triggering receptor expressed in myeloid cells 2, VEGF: vascular endothelial growth factor, WASP: Wiscott-Aldrich syndrome protein, WHO: World Health Organization, XIAP: X-linked inhibitor of apoptosis

References

    1. Public Health Agency of Canada: The chief public health officer’s report on the state of public health in Canada, 2014: Public health in the future. Public Health Agency of Canada, Ottawa (Canada) (2014)

    2. Statistics Canada: Population projections for Canada, provinces and territories 2009 to 2036. Statistics Canada, Ottawa (Canada) (2010)

    3. Statistics Canada: The Canadian population in 2011: age and sex. Statistics Canada, Ottawa (Canada) (2012)

    4. World Health Organization: Facts about ageing. World Health Organization (2014) (cited 2015 Aug 19) Available from:

    5. World Health Organization: The burden of musculoskeletal conditions at the start of the new millennium. World Health Organization Technical Report Series 919, World Health Organization, Geneva (Switzerland) (2003)

    6. Osteoporosis Canada: Facts & Statistics.Osteoporosis Canada (cited 2015 Aug 19) Available from: http://www.osteoporosis.ca/osteoporosis-and-you/osteoporosis-facts-and-statistics

    7. Ostéoporose Canada: Qu’est-ce que l’ostéoporose? Ostéoporose Canada (cited 2015 Aug 20) Available from: http://www.osteoporosecanada.ca/losteoporose-et-vous/quest-ce-que-losteoporose

    8. M.E. Marquis, E. Lord, E. Bergeron, O. Drevelle, H. Park, F. Cabana, H. Senta, N. Faucheux: Bone cells-biomaterials interactions. Front Biosci 14, 1023–67 (2009)

    9. S.K. Nilsson, H.M. Johnston, J.A. Coverdale: Spatial localization of transplanted hemopoietic stem cells: inferences for the localization of stem cell niches. Blood 97, 2293–9 (2001)

    10. P. V Giannoudis, H. Dinopoulos, E. Tsiridis: Bone substitutes: An update. Injury 36, S20–7 (2005)

    11. R. Dimitriou, G.I. Mataliotakis, A.G. Angoules, N.K. Kanakaris, P. V Giannoudis: Complications following autologous bone graft harvesting from the iliac crest and using the RIA: a systematic review. Injury 42 Supp l2, S3–15 (2011)

    12. R.J. Bruno, M.S. Cohen, A. Berzins, D.R. Sumner: Bone graft harvesting from the distal radius, olecranon, and iliac crest: a quantitative analysis. J Hand Surg Am 26, 135–41 (2001)

    13. J.D. Conway: Autograft and nonunions: morbidity with intramedullary bone graft versus iliac crest bone graft. Orthop Clin North Am 41, 75–84 (2010)

    14. A. Dumas, C. Gaudin-Audrain, G. Mabilleau, P. Massin, L. Hubert, M.F. Basle, D. Chappard: The influence of processes for the purification of human bone allografts on the matrix surface and cytocompatibility. Biomaterials 27, 4204–11 (2006)

    15. G.E. Friedlaender, D.M. Strong, W.W. Tomford, H.J. Mankin: Long-term follow-up of patients with osteochondral allografts. A correlation between immunologic responses and clinical outcome. Orthop Clin North Am 30, 583–8 (1999)

    16. G.E. Friedlaender: Bone allografts: the biological consequences of immunological events. J Bone Joint Surg Am 73, 1119–22 (1991)

    17. S.M. Graham, A. Leonidou, N. Aslam-Pervez, A. Hamza, P. Panteliadis, M. Heliotis, A. Mantalaris, E. Tsiridis: Biological therapy of bone defects: the immunology of bone allo-transplantation. Expert Opin Biol Ther 10, 885–901 (2010)

    18. R. Russo, N. Scarborough: Inactivation of viruses in demineralized bone matrix. FDA Work tissue Transplant Reprod tissue, p.20–1 (1995)

    19. L.L. Hench, J.M. Polak: Third-generation biomedical materials. Science 295, 1014–7 (2002)

    20. F. Monchau, A. Lefevre, M. Descamps, A. Belquin-myrdycz, P. Laffargue, H.F. Hildebrand: In vitro studies of human and rat osteoclast activity on hydroxyapatite, beta-tricalcium phosphate, calcium carbonate. Biomol Eng 19, 143–52 (2002)

    21. O. Drevelle, A. Daviau, M.A. Lauzon, N. Faucheux: Effect of BMP-2 and/or BMP-9 on preosteoblasts attached to polycaprolactone functionalized by adhesive peptides derived from bone sialoprotein. Biomaterials 34, 1051–62 (2013)

    22. Y.J. Lee, J.-H. Lee, H.-J. Cho, H.K. Kim, T.R. Yoon, H. Shin: Electrospun fibers immobilized with bone forming peptide-1 derived from BMP7 for guided bone regeneration. Biomaterials 34, 5059–69 (2013)

    23. P.C. Bessa, M. Casal, R.L. Reis: Bone morphogenetic proteins in tissue engineering: the road from the laboratory to the clinic, part I (basic concepts). J Tissue Eng Regen Med 2, 1–13 (2008)

    24. X. He, X. Yang, E. Jabbari: Combined effect of osteopontin and BMP-2 derived peptides grafted to an adhesive hydrogel on osteogenic and vasculogenic differentiation of marrow stromal cells. Langmuir 28, 5387–97 (2012)

    25. R.M. Ajiboye, J.T. Hamamoto, M.A. Eckardt, J.C. Wang: Clinical and radiographic outcomes of concentrated bone marrow aspirate with allograft and demineralized bone matrix for posterolateral and interbody lumbar fusion in elderly patients. Eur Spine J 24, 2567–72 (2015)

    26. L.S. Pryor, E. Gage, C.-J. Langevin, F. Herrera, A.D. Breithaupt, C.R. Gordon, A.M. Afifi, J.E. Zins, H. Meltzer, A. Gosman, S.R. Cohen, R. Holmes: Review of bone substitutes. Craniomaxillofac Trauma Reconstr 2, 151–60 (2009)

    27. X. He, J. Ma, E. Jabbari: Effect of grafting RGD and BMP-2 protein-derived peptides to a hydrogel substrate on osteogenic differentiation of marrow stromal cells. Langmuir 24, 12508–16 (2008)

    28. K. Lavery, P. Swain, D. Falb, M.H. Alaoui-Ismaili: BMP-2/4 and BMP-6/7 differentially utilize cell surface receptors to induce osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. J Biol Chem 283, 20948–58 (2008)

    29. T. Nakase, H. Yoshikawa: Potential roles of bone morphogenetic proteins (BMPs) in skeletal repair and regeneration. J Bone Miner Metab 24, 425–33 (2006)

    30. C.D. Reyes, A.J. Garcia: Alpha2beta1 integrin-specific collagen-mimetic surfaces supporting osteoblastic differentiation. J Biomed Mater Res A 69, 591–600 (2004)

    31. R.S. Carvalho, P.J. Kostenuik, E. Salih, A. Bumann, L.C. Gerstenfeld: Selective adhesion of osteoblastic cells to different integrin ligands induces osteopontin gene expression. Matrix Biol 22, 241–9 (2003)

    32. W.J. Boyle, W.S. Simonet, D.L. Lacey: Osteoclast differentiation and activation. Nature 423, 337–42 (2003)

    33. S.C. Manolagas: Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev 21, 115–37 (2000)

    34. M. Capulli, R. Paone, N. Rucci: Osteoblast and osteocyte: games without frontiers. Arch Biochem Biophys 561, 3–12 (2014)

    35. M.L. Knothe Tate, J.R. Adamson, A.E. Tami, T.W. Bauer: The osteocyte. Int J Biochem Cell Biol 36, 1–8 (2004)

    36. S. Weinbaum, S.C. Cowin, Y. Zeng: Amodel for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses. J Biomech 27, 339–60 (1994)

    37. L.E. Mulcahy, D. Taylor, T.C. Lee, G.P. Duffy: RANKL and OPG activity is regulated by injury size in networks of osteocyte-like cells. Bone 48, 182–8 (2011)

    38. O. Verborgt, G.J. Gibson, M.B. Schaffler: Loss of osteocyte integrity in association with microdamage and bone remodeling after fatigue in vivo. J Bone Miner Res 15, 60–7 (2000)

    39. Z.F. Jaworski, B. Duck, G. Sekaly: Kinetics of osteoclasts and their nuclei in evolving secondary Haversian systems. J Anat 133, 397–405 (1981)

    40. K. Vaananen: Mechanism of osteoclast mediated bone resorption-rationale for the design of new therapeutics. Adv Drug Deliv Rev 57, 959–71 (2005)

    41. S.A. Nesbitt, M.A. Horton: Trafficking of matrix collagens through bone-resorbing osteoclasts. Science 276, 266–9 (1997)

    42. S. Ory, H. Brazier, G. Pawlak, A. Blangy: Rho GTPases in osteoclasts: orchestrators of podosome arrangement. Eur J Cell Biol 87, 469–77 (2008)

    43. K. Gelse, E. Poschl, T. Aigner: Collagens-structure, function, and biosynthesis. Adv Drug Deliv Rev 55, 1531–46 (2003)

    44. J.P.R.O. Orgel, J.D. San Antonio, O. Antipova: Molecular and structural mapping of collagen fibril interactions. Connect Tissue Res 52, 2–17 (2011)

    45. T. Gurry, P.S. Nerenberg, C.M. Stultz: The contribution of interchain salt bridges to triple-helical stability in collagen. Biophys J 98, 2634–43 (2010)

    46. A. Gautieri, S. Vesentini, A. Redaelli, M.J. Buehler: Hierarchical structure and nanomechanics of collagen microfibrils from the atomistic scale up. Nano Lett 11, 757–66 (2011)

    47. L.W. Fisher, N.S. Fedarko: Six genes expressed in bones and teeth encode the current members of the SIBLING family of proteins. Connect Tissue Res 44 Suppl 1, 33–40 (2003)

    48. K.A. Staines, V.E. MacRae, C. Farquharson: The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling. J Endocrinol 214, 241–55 (2012)

    49. G.K. Hunter, H.A. Goldberg: Nucleation of hydroxyapatite by bone sialoprotein. Proc Natl Acad Sci U S A 90, 8562–5 (1993)

    50. W.N. Addison, D.L. Masica, J.J. Gray, M.D. McKee: Phosphorylation-dependent inhibition of mineralization by osteopontin ASARM peptides is regulated by PHEX cleavage. J Bone Miner Res 25, 695–705 (2010)

    51. W.J. Landis, R. Jacquet: Association of calcium and phosphate ions with collagen in the mineralization of vertebrate tissues. Calcif Tissue Int 93, 329–37 (2013)

    52. E. Holm, J.E. Aubin, G.K. Hunter, F. Beier, H.A. Goldberg: Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization. Bone 71, 145–54 (2015)

    53. E.H. Schwab, M. Halbig, K. Glenske, A.-S. Wagner, S. Wenisch, E.A. Cavalcanti-Adam: Distinct effects of RGD-glycoproteins on integrin-mediated adhesion and osteogenic differentiation of human mesenchymal stem cells. Int J Med Sci 10, 1846–59 (2013)

    54. A. Ode, G.N. Duda, J.D. Glaeser, G. Matziolis, S. Frauenschuh, C. Perka, C.J. Wilson, G. Kasper: Toward biomimetic materials in bone regeneration: functional behavior of mesenchymal stem cells on a broad spectrum of extracellular matrix components. J Biomed Mater Res A 95, 1114–24 (2010)

    55. R.T. Ingram, B.L. Clarke, L.W. Fisher, L.A. Fitzpatrick: Distribution of noncollagenous proteins in the matrix of adult human bone: evidence of anatomic and functional heterogeneity. J Bone Miner Res 8, 1019–29 (1993)

    56. A. Corsi, T. Xu, X.D. Chen, A. Boyde, J. Liang, M. Mankani, B. Sommer, R. VIozzo, I. Eichstetter, P.G. Robey, P. Bianco, M.F. Young: Phenotypic effects of biglycan deficiency are linked to collagen fibril abnormalities, are synergized by decorin deficiency, and mimic Ehlers-Danlos-like changes in bone and other connective tissues. J Bone Miner Res 17, 1180–9 (2002)

    57. L.E. Bertassoni, M. V Swain: The contribution of proteoglycans to the mechanical behavior of mineralized tissues. J Mech Behav Biomed Mater 38, 91–104 (2014)

    58. X.-D. Chen, L.W. Fisher, P.G. Robey, M.F. Young: The small leucine-rich proteoglycan biglycan modulates BMP-4-induced osteoblast differentiation. FASEB J 18, 948–58 (2004)

    59. I. Fernández-Tresguerres-Hernández-Gil, M.A. Alobera Gracia, M. del Canto Pingarrn, L. Blanco Jerez: Physiological bases of bone regeneration I. Histology and physiology of bone tissue. Med Oral 11, E47–51 (2006)

    60. A. Veis, J.R. Dorvee: Biomineralization mechanisms: a new paradigm for crystal nucleation in organic matrices. Calcif Tissue Int 93, 307–15 (2013)

    61. F. Nudelman, K. Pieterse, A. George, P.H.H. Bomans, H. Friedrich, L.J. Brylka, P.A.J. Hilbers, G. de With, N.A.J.M. Sommerdijk: The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors. Nat Mater 9, 1004–9 (2010)

    62. W.-J. Chung, K.-Y. Kwon, J. Song, S.-W. Lee: Evolutionary screening of collagen-like peptides that nucleate hydroxyapatite crystals. Langmuir 27, 7620–8 (2011)

    63. Z. Xu, Y. Yang, W. Zhao, Z. Wang, W.J. Landis, Q. Cui, N. Sahai: Molecular mechanisms for intrafibrillar collagen mineralization in skeletal tissues. Biomaterials 39, 59–66 (2015)

    64. Y. Wang, T. Azais, M. Robin, A. Vallee, C. Catania, P. Legriel, G. Pehau-Arnaudet, F. Babonneau, M.-M. Giraud-Guille, N. Nassif: The predominant role of collagen in the nucleation, growth, structure and orientation of bone apatite. Nat Mater 11, 724–33 (2012)

    65. W.L. Grayson, B.A. Bunnell, E. Martin, T. Frazier, B.P. Hung, J.M. Gimble: Stromal cells and stem cells in clinical bone regeneration. Nat Rev Endocrinol 11, 140–50 (2015)

    66. J. Kobolak, A. Dinnyes, A. Memic, A. Khademhosseini, A. Mobasheri: Mesenchymal stem cells: identification, phenotypic characterization, biological properties and potential for regenerative medicine through biomaterial micro engineering of their niche. Methods (in press) (2015)

    67. B.P. Hung, D.L. Hutton, K.L. Kozielski, C.J. Bishop, B. Naved, J.J. Green, A.I. Caplan, J.M. Gimble, A.H. Dorafshar, W.L. Grayson: Platelet-derived growth factor BB enhances osteogenesis of adipose-derived but not bone marrow-derived mesenchymal stromal/stem cells. Stem Cells 33, 2773–84 (2015)

    68. J. Baker, J.P. Liu, E.J. Robertson, A. Efstratiadis: Role of insulin-like growth factors in embryonic and postnatal growth. Cell 75, 73–82 (1993)

    69. N. Maegawa, K. Kawamura, M. Hirose, H. Yajima, Y. Takakura, H. Ohgushi: Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2). J Tissue Eng Regen Med 1, 306–13 (2007)

    70. H. Cheng, W. Jiang, F.M. Phillips, R.C. Haydon, Y. Peng, L. Zhou, H.H. Luu, N. An, B. Breyer, P. Vanichakarn, J.P. Szatkowski, J.Y. Park, T.C. He: Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am 85-A, 1544–52 (2003)

    71. C. Zhao, N. Irie, Y. Takada, K. Shimoda, T. Miyamoto, T. Nishiwaki, T. Suda, K. Matsuo: Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab 4, 111–21 (2006)

    72. T. Negishi-Koga, M. Shinohara, N. Komatsu, H. Bito, T. Kodama, R.H. Friedel, H. Takayanagi: Suppression of bone formation by osteoclastic expression of semaphorin 4D. Nat Med 17, 1473–80 (2011)

    73. Y.-M. Lee, N. Fujikado, H. Manaka, H. Yasuda, Y. Iwakura: IL-1 plays an important role in the bone metabolism under physiological conditions. Int Immunol 22, 805–16 (2010)

    74. T. Cohen, D. Nahari, L.W. Cerem, G. Neufeld, B.Z. Levi: Interleukin 6 induces the expression of vascular endothelial growth factor. J Biol Chem 271, 736–41 (1996)

    75. S. Akeel, A. El-Awady, K. Hussein, M. El-Refaey, M. Elsalanty, M. Sharawy, M. Al-Shabrawey: Recombinant bone morphogenetic protein-2 induces up-regulation of vascular endothelial growth factor and interleukin 6 in human pre-osteoblasts: role of reactive oxygen species. Arch Oral Biol 57, 445–52 (2012)

    76. E. Hay, A. Nouraud, P.J. Marie: N-cadherin negatively regulates osteoblast proliferation and survival by antagonizing Wnt, ERK and PI3K/Akt signalling. PLoS One 4, e8284 (2009)

    77. C.E. Clarkin, L.C. Gerstenfeld: VEGF and bone cell signalling: an essential vessel for communication? Cell Biochem Funct 31, 1–11 (2013)

    78. J. Street, B. Lenehan: Vascular endothelial growth factor regulates osteoblast survival - evidence for an autocrine feedback mechanism. J Orthop Surg Res 4, 19 (2009)

    79. H. Tokuda, S. Adachi, R. Matsushima-Nishiwaki, K. Kato, H. Natsume, T. Otsuka, O. Kozawa: Enhancement of basic fibroblast growth factor-stimulated VEGF synthesis by Wnt3a in osteoblasts. Int J Mol Med 27, 859–64 (2011)

    80. H. Natsume, H. Tokuda, R. Matsushima-Nishiwaki, K. Kato, K. Yamakawa, T. Otsuka, O. Kozawa: Wnt3a upregulates transforming growth factor-beta-stimulated VEGF synthesis in osteoblasts. Cell Biochem Funct 29, 371–7 (2011)

    81. D. Agas, M.G. Sabbieti, L. Marchetti, L. Xiao, M.M. Hurley: FGF-2 enhances Runx-2/Smads nuclear localization in BMP-2 canonical signaling in osteoblasts. J Cell Physiol 228, 2149–58 (2013)

    82. L.F. Charles, J.L. Woodman, D. Ueno, G. Gronowicz, M.M. Hurley, L.T. Kuhn: Effects of low dose FGF-2 and BMP-2 on healing of calvarial defects in old mice. Exp Gerontol 64, 62–9 (2015)

    83. B.-Z. Shilo: The regulation and functions of MAPK pathways in Drosophila. Methods 68, 151–9 (2014)

    84. H. Miraoui, K. Oudina, H. Petite, Y. Tanimoto, K. Moriyama, P.J. Marie: Fibroblast growth factor receptor 2 promotes osteogenic differentiation in mesenchymal cells via ERK1/2 and protein kinase C signaling. J Biol Chem 284, 4897–904 (2009)

    85. P. Marie: Differenciation, function and regulation of osteoblast. Medecine/Sciences 17, 1252–9 (2001)

    86. S. Suttapreyasri, S. Koontongkaew, A. Phongdara, U. Leggat: Expression of bone morphogenetic proteins in normal human intramembranous and endochondral bones. Int J Oral Maxillofac Surg 35, 444–52 (2006)

    87. M.R. Urist: Bone: formation by autoinduction. Science 150, 893–9 (1965)

    88. E. Lou: Bone morphogenetic proteins: an overview of therapeutic applications. Orthopedics 24, 501–4 (2001)

    89. A.H. Reddi: BMPs: from bone morphogenetic proteins to body morphogenetic proteins. Cytokine Growth Factor Rev 16, 249–50 (2005)

    90. J.M. Wozney, V. Rosen, A.J. Celeste, L.M. Mitsock, M.J. Whitters, R.W. Kriz, R.M. Hewick, E.A. Wang: Novel regulators of bone formation: molecular clones and activities. Science 242, 1528–34 (1988)

    91. Q. Kang, M.H. Sun, H. Cheng, Y. Peng, A.G. Montag, A.T. Deyrup, W. Jiang, H.H. Luu, J. Luo, J.P. Szatkowski, P. Vanichakarn, J.Y. Park, Y. Li, R.C. Haydon, T.C. He: Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Gene Ther 11, 1312–20 (2004)

    92. M. Kawabata, T. Imamura, K. Miyazono: Signal transduction by bone morphogenetic proteins. Cytokine Growth Factor Rev 9, 49–61 (1998)

    93. J.-H. Shim, M.B. Greenblatt, M. Xie, M.D. Schneider, W. Zou, B. Zhai, S. Gygi, L.H. Glimcher: TAK1 is an essential regulator of BMP signalling in cartilage. EMBO J 28, 2028–41 (2009)

    94. K. Miyazono: Signal transduction by bone morphogenetic protein receptors: functional roles of Smad proteins. Bone 25, 91–3 (1999)

    95. T. Matsubara, K. Kida, A. Yamaguchi, K. Hata, F. Ichida, H. Meguro, H. Aburatani, R. Nishimura, T. Yoneda: BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J Biol Chem 283, 29119–25 (2008)

    96. D.H. Walker, N.M. Wright: Bone morphogenetic proteins and spinal fusion. Neurosurg Focus 13, 1–13 (2002)

    97. H. Liu, Y. Liu, M. Viggeswarapu, Z. Zheng, L. Titus, S.D. Boden: Activation of c-Jun NH(2)-terminal kinase 1 increases cellular responsiveness to BMP-2 and decreases binding of inhibitory Smad6 to the type1 BMP receptor. J Bone Miner Res 26, 1122–32 (2011)

    98. L.B. Zimmerman, J.M. De Jesús-Escobar, R.M. Harland: The Spemann organizer signal noggin binds and inactivates bone morphogenetic protein 4. Cell 86, 599–606 (1996)

    99. S. Piccolo, Y. Sasai, B. Lu, E.M. De Robertis: Dorsoventral patterning in Xenopus: inhibition of ventral signals by direct binding of chordin to BMP-4. Cell 86, 589–98 (1996)

    100. D.P. Brazil, R.H. Church, S. Surae, C. Godson, F. Martin: BMP signalling: agony and antagony in the family. Trends Cell Biol 25, 249–64 (2015)

    101. D. Onichtchouk, Y.G. Chen, R. Dosch, V. Gawantka, H. Delius, J. Massague, C. Niehrs: Silencing of TGF-beta signalling by the pseudoreceptor BAMBI. Nature 401, 480–5 (1999)

    102. S. Souchelnytskyi, T. Nakayama, A. Nakao, A. Moren, C.H. Heldin, J.L. Christian, P. ten Dijke: Physical and functional interaction of murine and Xenopus Smad7 with bone morphogenetic protein receptors and transforming growth factor-beta receptors. J Biol Chem 273, 25364–70 (1998)

    103. T. Imamura, M. Takase, A. Nishihara, E. Oeda, J. Hanai, M. Kawabata, K. Miyazono: Smad6 inhibits signalling by the TGF-beta superfamily. Nature 389, 622–6 (1997)

    104. X. Duan, Y.-Y. Liang, X.-H. Feng, X. Lin: Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone morphogenetic protein signaling pathway. J Biol Chem 281, 36526–32 (2006)

    105. K. Yamaguchi, S. Nagai, J. Ninomiya-Tsuji, M. Nishita, K. Tamai, K. Irie, N. Ueno, E. Nishida, H. Shibuya, K. Matsumoto: XIAP, a cellular member of the inhibitor of apoptosis protein family, links the receptors to TAB1–TAK1 in the BMP signaling pathway. EMBO J 18, 179–87 (1999)

    106. Y. Liu, E.G. Shepherd, L.D. Nelin: MAPK phosphatases—regulating the immune response. Nat Rev Immunol 7, 202–12 (2007)

    107. D.J. Xu, Y.Z. Zhao, J. Wang, J.W. He, Y.G. Weng, J.Y. Luo: Smads, p38 and ERK1/2 are involved in BMP9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells. BMB Rep 45, 247–52 (2012)

    108. M.-A. Lauzon, A. Daviau, O. Drevelle, B. Marcos, N. Faucheux: Identification of a growth factor mimicking the synergistic effect of fetal bovine serum on BMP-9 cell response. Tissue Eng Part A 20, 2524–35 (2014)

    109. M. Kretzschmar, J. Doody, J. Massague: Opposing BMP and EGF signalling pathways converge on the TGF-beta family mediator Smad1. Nature 389, 618–22 (1997)

    110. H. Mitchell, A. Choudhury, R.E. Pagano, E.B. Leof: Ligand-dependent and -independent transforming growth factor-beta receptor recycling regulated by clathrin-mediated endocytosis and Rab11. Mol Biol Cell 15, 4166–78 (2004)

    111. Y.-G. Chen: Endocytic regulation of TGF-beta signaling. Cell Res 19, 58–70 (2009)

    112. T. Shen, C. Sun, Z. Zhang, N. Xu, X. Duan, X.-H. Feng, X. Lin: Specific control of BMP signaling and mesenchymal differentiation by cytoplasmic phosphatase PPM1H. Cell Res 24, 727–41 (2014)

    113. T. Naganawa, L. Xiao, J.D. Coffin, T. Doetschman, M.G. Sabbieti, D. Agas, M.M. Hurley: Reduced expression and function of bone morphogenetic protein-2 in bones of Fgf2 null mice. J Cell Biochem 103, 1975–88 (2008)

    114. X. Liu, J. Qin, Q. Luo, Y. Bi, G. Zhu, W. Jiang, S.H. Kim, M. Li, Y. Su, G. Nan, J. Cui, W. Zhang, R. Li, X. Chen, Y. Kong, J. Zhang, J. Wang, M.R. Rogers, H. Zhang, W. Shui, C. Zhao, N. Wang, X. Liang, N. Wu, Y. He, H.H. Luu, R.C. Haydon, L.L. Shi, T. Li, T.-C. He, M. Li: Cross-talk between EGF and BMP9 signalling pathways regulates the osteogenic differentiation of mesenchymal stem cells. J Cell Mol Med 17, 1160–72 (2013)

    115. G. Luther, E. R Wagner, G. Zhu, Q. Kang, Q. Luo, J. Lamplot, Y. Bi, X. Luo, J. Luo, C. Teven: BMP-9 induced osteogenic differentiation of mesenchymal stem cells: molecular mechanism and therapeutic potential. Curr Gene Ther 11, 229–40 (2011)

    116. J.F. Chau, W.F. Leong, B. Li: Signaling pathways governing osteoblast proliferation, differentiation and function. Histol Histopathol 24, 1593–606 (2009)

    117. W.P. Cawthorn, A.J. Bree, Y. Yao, B. Du, N. Hemati, G. Martinez-Santibanez, O.A. MacDougald: Wnt6, Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a beta-catenin-dependent mechanism. Bone 50, 477–89 (2012)

    118. Y.-D. Cho, W.-J. Kim, W.-J. Yoon, K.-M. Woo, J.-H. Baek, G. Lee, G.-S. Kim, H.-M. Ryoo: Wnt3a stimulates Mepe, matrix extracellular phosphoglycoprotein, expression directly by the activation of the canonical Wnt signaling pathway and indirectly through the stimulation of autocrine Bmp-2 expression. J Cell Physiol 227, 2287–96 (2012)

    119. R. Baron, M. Kneissel: WNT signaling in bone homeostasis and disease: from human mutations to treatments. Nat Med 19, 179–92 (2013)

    120. T. Gaur, C.J. Lengner, H. Hovhannisyan, R.A. Bhat, P.V.N. Bodine, B.S. Komm, A. Javed, A.J. van Wijnen, J.L. Stein, G.S. Stein, J.B. Lian: Canonical WNT signaling promotes osteogenesis by directly stimulating Runx2 gene expression. J Biol Chem 280, 33132–40 (2005)

    121. N. Tang, W.-X. Song, J. Luo, X. Luo, J. Chen, K.A. Sharff, Y. Bi, B.-C. He, J.-Y. Huang, G.-H. Zhu, Y.-X. Su, W. Jiang, M. Tang, Y. He, Y. Wang, L. Chen, G.-W. Zuo, J. Shen, X. Pan, R.R. Reid, H.H. Luu, R.C. Haydon, T.-C. He: BMP-9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/beta-catenin signalling. J Cell Mol Med 13, 2448–64 (2009)

    122. M. Plaisant, C. Fontaine, W. Cousin, N. Rochet, C. Dani, P. Peraldi: Activation of hedgehog signaling inhibits osteoblast differentiation of human mesenchymal stem cells. Stem Cells 27, 703–13 (2009)

    123. S. Spinella-Jaegle, G. Rawadi, S. Kawai, S. Gallea, C. Faucheu, P. Mollat, B. Courtois, B. Bergaud, V. Ramez, A.M. Blanchet, G. Adelmant, R. Baron, S. Roman-Roman: Sonic hedgehog increases the commitment of pluripotent mesenchymal cells into the osteoblastic lineage and abolishes adipocytic differentiation. J Cell Sci 114, 2085–94 (2001)

    124. G.L. Lin, K.D. Hankenson: Integration of BMP, Wnt, and notch signaling pathways in osteoblast differentiation. J Cell Biochem 112, 3491–501 (2011)

    125. H. Hu, M.J. Hilton, X. Tu, K. Yu, D.M. Ornitz, F. Long: Sequential roles of Hedgehog and Wnt signaling in osteoblast development. Development 132, 49–60 (2005)

    126. G. Sapkota, C. Alarcon, F.M. Spagnoli, A.H. Brivanlou, J. Massague: Balancing BMP signaling through integrated inputs into the Smad1 linker. Mol Cell 25, 441–54 (2007)

    127. J.-H. Lee, B.-G. Kim, J.-M. Ahn, H.-J. Park, S.-K. Park, J.-S. Yoo, J.R. 3rd Yates, J.-Y. Cho: Role of PI3K on the regulation of BMP2-induced beta-Catenin activation in human bone marrow stem cells. Bone 46, 1522–32 (2010)

    128. F. Ugarte, M. Ryser, S. Thieme, F.A. Fierro, K. Navratiel, M. Bornhäuser, S. Brenner: Notch signaling enhances osteogenic differentiation while inhibiting adipogenesis in primary human bone marrow stromal cells. Exp Hematol 37, 867–75. e1 (2009)

    129. K.A. Sharff, W.-X. Song, X. Luo, N. Tang, J. Luo, J. Chen, Y. Bi, B.-C. He, J. Huang, X. Li, W. Jiang, G.-H. Zhu, Y. Su, Y. He, J. Shen, Y. Wang, L. Chen, G.-W. Zuo, B. Liu, X. Pan, R.R. Reid, H.H. Luu, R.C. Haydon, T.-C. He: Hey1 basic helix-loop-helix protein plays an important role in mediating BMP9-induced osteogenic differentiation of mesenchymal progenitor cells. J Biol Chem 284, 649–59 (2009)

    130. D.S. de Jong, W.T. Steegenga, J.M.A. Hendriks, E.J.J. van Zoelen, W. Olijve, K.J. Dechering: Regulation of Notch signaling genes during BMP2-induced differentiation of osteoblast precursor cells. Biochem Biophys Res Commun 320, 100–7 (2004)

    131. N. Zamurovic, D. Cappellen, D. Rohner, M. Susa: Coordinated activation of notch, Wnt, and transforming growth factor-beta signaling pathways in bone morphogenic protein 2-induced osteogenesis. Notch target gene Hey1 inhibits mineralization and Runx2 transcriptional activity. J Biol Chem 279, 37704–15 (2004)

    132. H. Hojo, S. Ohba, F. Yano, T. Saito, T. Ikeda, K. Nakajima, Y. Komiyama, N. Nakagata, K. Suzuki, T. Takato, H. Kawaguchi, U. Chung: Gli1 protein participates in Hedgehog-mediated specification of osteoblast lineage during endochondral ossification. J Biol Chem 287, 17860–9 (2012)

    133. J.M. Hodge, M.A. Kirkland, G.C. Nicholson: Multiple roles of M-CSF in human osteoclastogenesis. J Cell Biochem 102, 759–68 (2007)

    134. T. Tsurukai, N. Udagawa, K. Matsuzaki, N. Takahashi, T. Suda: Roles of macrophage-colony stimulating factor and osteoclast differentiation factor in osteoclastogenesis. J Bone Miner Metab 18, 177–84 (2000)

    135. T. Wada, T. Nakashima, N. Hiroshi, J.M. Penninger: RANKL-RANK signaling in osteoclastogenesis and bone disease. Trends Mol Med 12, 17–25 (2006)

    136. M. Ishii, J.G. Egen, F. Klauschen, M. Meier-Schellersheim, Y. Saeki, J. Vacher, R.L. Proia, R.N. Germain: Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis. Nature 458, 524–8 (2009)

    137. T. Nakashima, M. Hayashi, T. Fukunaga, K. Kurata, M. Oh-Hora, J.Q. Feng, L.F. Bonewald, T. Kodama, A. Wutz, E.F. Wagner, J.M. Penninger, H. Takayanagi: Evidence for osteocyte regulation of bone homeostasis through RANKL expression. Nat Med 17, 1231–4 (2011)

    138. E.C. Weir, C.W. Lowik, I. Paliwal, K.L. Insogna: Colony stimulating factor-1 plays a role in osteoclast formation and function in bone resorption induced by parathyroid hormone and parathyroid hormone-related protein. J Bone Miner Res 11, 1474–81 (1996)

    139. J.W. Pike, S.M. Lee, M.B. Meyer: Regulation of gene expression by 1,25-dihydroxyvitamin D3 in bone cells: exploiting new approaches and defining new mechanisms. Bonekey Rep 3, 482 (2014)

    140. H. Takayanagi, K. Ogasawara, S. Hida, T. Chiba, S. Murata, K. Sato, A. Takaoka, T. Yokochi, H. Oda, K. Tanaka, K. Nakamura, T. Taniguchi: T-cell-mediated regulation of osteoclastogenesis by signalling cross-talk between RANKL and IFN-gamma. Nature 408, 600–5 (2000)

    141. F.P. Ross: M-CSF, c-Fms, and signaling in osteoclasts and their precursors. Ann N Y Acad Sci 1068, 110–6 (2006)

    142. F. Xu, S.L. Teitelbaum: Osteoclasts: New Insights. Bone Res 1, 11–26 (2013)

    143. S. Amano, Y.-T. Chang, Y. Fukui: ERK5 activation is essential for osteoclast differentiation. PLoS One 10, e0125054 (2015)

    144. H. Yasuda, N. Shima, N. Nakagawa, K. Yamaguchi, M. Kinosaki, S. Mochizuki, A. Tomoyasu, K. Yano, M. Goto, A. Murakami, E. Tsuda, T. Morinaga, K. Higashio, N. Udagawa, N. Takahashi, T. Suda: Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci U S A 95, 3597–602 (1998)

    145. M. Shinohara, H. Takayanagi: Novel osteoclast signaling mechanisms. Curr Osteoporos Rep 5, 67–72 (2007)

    146. T. Oikawa, Y. Kuroda, K. Matsuo: Regulation of osteoclasts by membrane-derived lipid mediators. Cell Mol Life Sci 70, 3341–53 (2013)

    147. H. Takayanagi, S. Kim, T. Koga, H. Nishina, M. Isshiki, H. Yoshida, A. Saiura, M. Isobe, T. Yokochi, J. Inoue, E.F. Wagner, T.W. Mak, T. Kodama, T. Taniguchi: Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell 3, 889–901 (2002)

    148. T. Kukita, N. Wada, A. Kukita, T. Kakimoto, F. Sandra, K. Toh, K. Nagata, T. Iijima, M. Horiuchi, H. Matsusaki, K. Hieshima, O. Yoshie, H. Nomiyama: RANKL-induced DC-STAMP is essential for osteoclastogenesis. J Exp Med 200, 941–6 (2004)

    149. T. Koga, M. Inui, K. Inoue, S. Kim, A. Suematsu, E. Kobayashi, T. Iwata, H. Ohnishi, T. Matozaki, T. Kodama, T. Taniguchi, H. Takayanagi, T. Takai: Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature 428, 758–63 (2004)

    150. N. Takahashi, N. Udagawa, T. Suda: Vitamin D endocrine system and osteoclasts. Bonekey Rep 3, 495 (2014)

    151. A.D. Barrow, N. Raynal, T.L. Andersen, D.A. Slatter, D. Bihan, N. Pugh, M. Cella, T. Kim, J. Rho, T. Negishi-Koga, J.-M. Delaisse, H. Takayanagi, J. Lorenzo, M. Colonna, R.W. Farndale, Y. Choi, J. Trowsdale: OSCAR is a collagen receptor that costimulates osteoclastogenesis in DAP12-deficient humans and mice. J Clin Invest 121, 3505–16 (2011)

    152. S. Li, C.H. Miller, E. Giannopoulou, X. Hu, L.B. Ivashkiv, B. Zhao: RBP-J imposes a requirement for ITAM-mediated costimulation of osteoclastogenesis. J Clin Invest 124, 5057–73 (2014)

    153. B. Zhao, S.N. Grimes, S. Li, X. Hu, L.B. Ivashkiv: TNF-induced osteoclastogenesis and inflammatory bone resorption are inhibited by transcription factor RBP-J. J Exp Med 209, 319–34 (2012)

    154. C.L. Long, M.B. Humphrey: Osteoimmunology: the expanding role of immunoreceptors in osteoclasts and bone remodeling. Bonekey Rep 1, (2012)

    155. H. Zhao, F. Patrick Ross: Mechanisms of osteoclastic secretion. Ann N Y Acad Sci 1116, 238–44 (2007)

    156. H.K. Vaananen, T. Laitala-Leinonen: Osteoclast lineage and function. Arch Biochem Biophys 473, 132–8 (2008)

    157. A. Broege, L. Pham, E.D. Jensen, A. Emery, T.-H. Huang, M. Stemig, H. Beppu, A. Petryk, M. O’Connor, K. Mansky, R. Gopalakrishnan: Bone morphogenetic proteins signal via SMAD and mitogen-activated protein (MAP) kinase pathways at distinct times during osteoclastogenesis. J Biol Chem 288, 37230–40 (2013)

    158. K. Itoh, N. Udagawa, T. Katagiri, S. Iemura, N. Ueno, H. Yasuda, K. Higashio, J.M. Quinn, M.T. Gillespie, T.J. Martin, T. Suda, N. Takahashi: Bone morphogenetic protein 2 stimulates osteoclast differentiation and survival supported by receptor activator of nuclear factor-kappaB ligand. Endocrinology 142, 3656–62 (2001)

    159. E.D. Jensen, L. Pham, C.J.J. Billington, K. Espe, A.E. Carlson, J.J. Westendorf, A. Petryk, R. Gopalakrishnan, K. Mansky: Bone morphogenic protein 2 directly enhances differentiation of murine osteoclast precursors. J Cell Biochem 109, 672–82 (2010)

    160. D. Fong, M. Bisson, G. Laberge, S. McManus, G. Grenier, N. Faucheux, S. Roux: Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro. Cell Signal 25, 717–28 (2013)

    161. T.A. Einhorn, L.C. Gerstenfeld: Fracture healing: mechanisms and interventions. Nat Rev Rheumatol 11, 45–54 (2015)

    162. A. Ozaki, M. Tsunoda, S. Kinoshita, R. Saura: Role of fracture hematoma and periosteum during fracture healing in rats: interaction of fracture hematoma and the periosteum in the initial step of the healing process. J Orthop Sci 5, 64–70 (2000)

    163. Z.S. Ai-Aql, A.S. Alagl, D.T. Graves, L.C. Gerstenfeld, T.A. Einhorn: Molecular mechanisms controlling bone formation during fracture healing and distraction osteogenesis. J Dent Res 87, 107–18 (2008)

    164. X. Yang, B.F. Ricciardi, A. Hernandez-Soria, Y. Shi, N. Pleshko Camacho, M.P.G. Bostrom: Callus mineralization and maturation are delayed during fracture healing in interleukin-6 knockout mice. Bone 41, 928–36 (2007)

    165. X. Du, Y. Xie, C.J. Xian, L. Chen: Role of FGFs/FGFRs in skeletal development and bone regeneration. J Cell Physiol 227, 3731–43 (2012)

    166. E.A. Gorter, N.A.T. Hamdy, N.M. Appelman-Dijkstra, I.B. Schipper: The role of vitamin D in human fracture healing: a systematic review of the literature. Bone 64, 288–97 (2014)

    167. Y.Y. Yu, S. Lieu, C. Lu, C. Colnot: Bone morphogenetic protein 2 stimulates endochondral ossification by regulating periosteal cell fate during bone repair. Bone 47, 65–73 (2010)

    168. C. Gabay: Interleukin-6 and chronic inflammation. Arthritis Res Ther 8 Suppl 2, S3 (2006)

    169. J. Scheller, A. Chalaris, D. Schmidt-Arras, S. Rose-John: The pro-and anti-inflammatory properties of the cytokine interleukin-6. Biochim Biophys Acta 1813, 878–88 (2011)

    170. S. Dekel, G. Lenthall, M.J. Francis: Release of prostaglandins from bone and muscle after tibial fracture. An experimental study in rabbits. J Bone Joint Surg Br 63-B, 185–9 (1981)

    171. B. Shen, A. Wei, H. Tao, A.D. Diwan, D.D.F. Ma: BMP-2 enhances TGF-beta3-mediated chondrogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in alginate bead culture. Tissue Eng Part A 15, 1311–20 (2009)

    172. N.L. Hedgecock, T. Hadi, A.A. Chen, S.B. Curtiss, R.B. Martin, S.J. Hazelwood: Quantitative regional associations between remodeling, modeling, and osteocyte apoptosis and density in rabbit tibial midshafts. Bone 40, 627–37 (2007)

    173. G. Kogianni, V. Mann, B.S. Noble: Apoptotic bodies convey activity capable of initiating osteoclastogenesis and localized bone destruction. J Bone Miner Res 23, 915–27 (2008)

    174. T.J. Heino, T.A. Hentunen, H.K. Vaananen: Osteocytes inhibit osteoclastic bone resorption through transforming growth factor-beta: enhancement by estrogen. J Cell Biochem 85, 185–97 (2002)

    175. R.O. Hynes: Integrins: bidirectional, allosteric signaling machines. Cell 110, 673–87 (2002)

    176. E. Ruoslahti, M.D. Pierschbacher: New perspectives in cell adhesion: RGD and integrins. Science 238, 491–7 (1987)

    177. A. Jikko, S.E. Harris, D. Chen, D.L. Mendrick, C.H. Damsky: Collagen integrin receptors regulate early osteoblast differentiation induced by BMP-2. J Bone Miner Res 14, 1075–83 (1999)

    178. R. Zaidel-Bar, M. Cohen, L. Addadi, B. Geiger: Hierarchical assembly of cell-matrix adhesion complexes. Biochem Soc Trans 32, 416–20 (2004)

    179. M.A. Schwartz: Integrins and extracellular matrix in mechanotransduction. Cold Spring Harb Perspect Biol 2, a005066 (2010)

    180. R. Zaidel-Bar, B. Geiger: The switchable integrin adhesome. J Cell Sci 123, 1385–8 (2010)

    181. S. Huveneers, E.H.J. Danen: Adhesion signaling - crosstalk between integrins, Src and Rho. J Cell Sci 122, 1059–69 (2009)

    182. H.B. Schiller, C.C. Friedel, C. Boulegue, R. Fassler: Quantitative proteomics of the integrin adhesome show a myosin II-dependent recruitment of LIM domain proteins. EMBO Rep 12, 259–66 (2011)

    183. J.-C. Kuo, X. Han, C.-T. Hsiao, J.R. 3rdYates, C.M. Waterman: Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for beta-Pix in negative regulation of focal adhesion maturation. Nat Cell Biol 13, 383–93 (2011)

    184. J.D. Humphries, A. Byron, M.D. Bass, S.E. Craig, J.W. Pinney, D. Knight, M.J. Humphries: Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci Signal 2, ra51 (2009)

    185. T. Geiger, R. Zaidel-Bar: Opening the floodgates: proteomics and the integrin adhesome. Curr Opin Cell Biol 24, 562–8 (2012)

    186. J. Robertson, G. Jacquemet, A. Byron, M.C. Jones, S. Warwood, J.N. Selley, D. Knight, J.D. Humphries, M.J. Humphries: Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun 6, 6265 (2015)

    187. J.-E. Hoffmann, Y. Fermin, R. Lo Stricker, K. Ickstadt, E. Zamir: Symmetric exchange of multi-protein building blocks between stationary focal adhesions and the cytosol. Elife 3, e02257 (2014)

    188. N. Elad, T. Volberg, I. Patla, V. Hirschfeld-Warneken, C. Grashoff, J.P. Spatz, R. Fassler, B. Geiger, O. Medalia: The role of integrin-linked kinase in the molecular architecture of focal adhesions. J Cell Sci 126, 4099–107 (2013)

    189. J.D. Humphries, P. Wang, C. Streuli, B. Geiger, M.J. Humphries, C. Ballestrem: Vinculin controls focal adhesion formation by direct interactions with talin and actin. J Cell Biol 179, 1043–57 (2007)

    190. S.J. Shattil, C. Kim, M.H. Ginsberg: The final steps of integrin activation: the end game. Nat Rev Mol Cell Biol 11, 288–300 (2010)

    191. D.D. Schlaepfer, C.R. Hauck, D.J. Sieg: Signaling through focal adhesion kinase. Prog Biophys Mol Biol 71, 435–78 (1999)

    192. M.D. Schaller: Biochemical signals and biological responses elicited by the focal adhesion kinase. Biochim Biophys Acta 1540, 1–21 (2001)

    193. M.C. Frame, H. Patel, B. Serrels, D. Lietha, M.J. Eck: The FERM domain: organizing the structure and function of FAK. Nat Rev cell Biol 11, 802–14 (2010)

    194. C.T. Mierke: The role of focal adhesion kinase in the regulation of cellular mechanical properties. Phys Biol 10, 65005 (2013)

    195. D. Lietha, X. Cai, D.F.J. Ceccarelli, Y. Li, M.D. Schaller, M.J. Eck: Structural basis for the autoinhibition of focal adhesion kinase. Cell 129, 1177–87 (2007)

    196. A. Tomar, D.D. Schlaepfer: Focal adhesion kinase: switching between GAPs and GEFs in the regulation of cell motility. Curr Opin Cell Biol 21, 676–83 (2009)

    197. M.D. Schaller, J.T. Parsons: Focal adhesion kinase and associated proteins. Curr Opin Cell Biol 6, 705–10 (1994)

    198. J.E. Sero, A.E. German, A. Mammoto, D.E. Ingber: Paxillin controls directional cell motility in response to physical cues. Cell Adh Migr 6, 502–8 (2012)

    199. B. Geiger, A. Bershadsky, R. Pankov, K.M. Yamada: Transmembrane crosstalk between the extracellular matrix-cytoskeleton crosstalk. Nat Rev cell Biol 2, 793–805 (2001)

    200. D.R. Critchley: Biochemical and structural properties of the integrin-associated cytoskeletal protein talin. Annu Rev Biophys 38, 235–54 (2009)

    201. E. Zamir, M. Katz, Y. Posen, N. Erez, K.M. Yamada, B.-Z. Katz, S. Lin, D.C. Lin, A. Bershadsky, Z. Kam, B. Geiger: Dynamics and segregation of cell–matrix adhesions in cultured fibroblasts. Nat Cell Biol 2, 191–6 (2000)

    202. B.Z. Katz, E. Zamir, A. Bershadsky, Z. Kam, K.M. Yamada, B. Geiger: Physical state of the extracellular matrix regulates the structure and molecular composition of cell-matrix adhesions. Mol Biol Cell 11, 1047–60 (2000)

    203. M. Lin, H. Wang, C. Ruan, J. Xing, J. Wang, Y. Li, Y. Wang, Y. Luo: Adsorption force of fibronectin on various surface chemistries and its vital role in osteoblast adhesion. Biomacromolecules 16, 973–84 (2015)

    204. K.M. Yamada, R. Pankov, E. Cukierman: Dimensions and dynamics in integrin function. Braz J Med Biol Res 36, 959–66 (2003)

    205. L.H. Golden, K.L. Insogna: The expanding role of PI3-kinase in bone. Bone 34, 3–12 (2004)

    206. S.B. Rodan, G.A. Rodan: Integrin function in osteoclasts. J Endocrinol 154 Suppl, S47–56 (1997)

    207. R. Faccio, S. Takeshita, A. Zallone, F.P. Ross, S.L. Teitelbaum: c-Fms and the alphavbeta3 integrin collaborate during osteoclast differentiation. J Clin Invest 111, 749–58 (2003)

    208. S.M. Frisch, R.A. Screaton: Anoikis mechanisms. Curr Opin Cell Biol 13, 555–62 (2001)

    209. D.G. Stupack, X.S. Puente, S. Boutsaboualoy, C.M. Storgard, D.A. Cheresh: Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins. J Cell Biol 155, 459–70 (2001)

    210. H. Zhao, F.P. Ross, S.L. Teitelbaum: Unoccupied alpha(v)beta3 integrin regulates osteoclast apoptosis by transmitting a positive death signal. Mol Endocrinol 19, 771–80 (2005)

    211. H. Schachtner, S.D.J. Calaminus, S.G. Thomas, L.M. Machesky: Podosomes in adhesion, migration, mechanosensing and matrix remodeling. Cytoskeleton (Hoboken) 70, 572–89 (2013)

    212. N. Takegahara, S. Kang, S. Nojima, H. Takamatsu, T. Okuno, H. Kikutani, T. Toyofuku, A. Kumanogoh: Integral roles of a guanine nucleotide exchange factor, FARP2, in osteoclast podosome rearrangements. FASEB J 24, 4782–92 (2010)

    213. F. Saltel, A. Chabadel, E. Bonnelye, P. Jurdic: Actin cytoskeletal organisation in osteoclasts: a model to decipher transmigration and matrix degradation. Eur J Cell Biol 87, 459–68 (2008)

    214. P. Hotulainen, P. Lappalainen: Stress fibers are generated by two distinct actin assembly mechanisms in motile cells. J Cell Biol 173, 383–94 (2006)

    215. T. Uruno, J. Liu, Y. Li, N. Smith, X. Zhan: Sequential interaction of actin-related proteins 2 and 3 (Arp2/3) complex with neural Wiscott-Aldrich syndrome protein (N-WASP) and cortactin during branched actin filament network formation. J Biol Chem 278, 26086–93 (2003)

    216. C. Itzstein, F.P. Coxon, M.J. Rogers: The regulation of osteoclast function and bone resorption by small GTPases. Small GTPases 2, 117–30 (2011)

    217. O. Destaing, F. Saltel, J.-C. Geminard, P. Jurdic, F. Bard: Podosomes display actin turnover and dynamic self-organization in osteoclasts expressing actin-green fluorescent protein. Mol Biol Cell 14, 407–16 (2003)

    218. C. Luxenburg, L. Addadi, B. Geiger: The molecular dynamics of osteoclast adhesions. Eur J Cell Biol 85, 203–11 (2006)

    219. H. Touaitahuata, E. Planus, C. Albiges-Rizo, A. Blangy, G. Pawlak: Podosomes are dispensable for osteoclast differentiation and migration. Eur J Cell Biol 92, 139–49 (2013)

    220. P. Jurdic, F. Saltel, A. Chabadel, O. Destaing: Podosome and sealing zone: specificity of the osteoclast model. Eur J Cell Biol 85, 195–202 (2006)

    221. S. Hu, E. Planus, D. Georgess, C. Place, X. Wang, C. Albiges-Rizo, P. Jurdic, J.-C. Geminard: Podosome rings generate forces that drive saltatory osteoclast migration. Mol Biol Cell 22, 3120–6 (2011)

    222. C.F. Lai, S.L. Cheng: Alphavbeta integrins play an essential role in BMP-2 induction of osteoblast differentiation. J Bone Miner Res 20, 330–40 (2005)

    223. A.R. Reynolds, I.R. Hart, A.R. Watson, J.C. Welti, R.G. Silva, S.D. Robinson, G. Da Violante, M. Gourlaouen, M. Salih, M.C. Jones, D.T. Jones, G. Saunders, V. Kostourou, F. Perron-Sierra, J.C. Norman, G.C. Tucker, K.M. Hodivala-Dilke: Stimulation of tumor growth and angiogenesis by low concentrations of RGD-mimetic integrin inhibitors. Nat Med 15, 392–400 (2009)

    224. H.L. Goel, M. Breen, J. Zhang, I. Das, S. Aznavoorian-Cheshire, N.M. Greenberg, A. Elgavish, L.R. Languino: beta1A integrin expression is required for type1 insulin-like growth factor receptor mitogenic and transforming activities and localization to focal contacts. Cancer Res 65, 6692–700 (2005)

    225. B.P. Eliceiri: Integrin and growth factor receptor crosstalk. Circ Res 89, 1104–10 (2001)

    226. J. Veevers-Lowe, S.G. Ball, A. Shuttleworth, C.M. Kielty: Mesenchymal stem cell migration is regulated by fibronectin through alpha5beta1-integrin-mediated activation of PDGFR-beta and potentiation of growth factor signals. J Cell Sci 124, 1288–300 (2011)

    227. T.-H. Chen, P.-C. Chan, C.-L. Chen, H.-C. Chen: Phosphorylation of focal adhesion kinase on tyrosine 194 by Met leads to its activation through relief of autoinhibition. Oncogene 30, 153–66 (2011)

    228. Y. Jung, J.H. McCarty: Band 4.1. proteins regulate integrin-dependent cell spreading. Biochem Biophys Res Commun 426, 578–84 (2012)

    229. M. Higuchi, R. Kihara, T. Okazaki, I. Aoki, S. Suetsugu, Y. Gotoh: Akt1 promotes focal adhesion disassembly and cell motility through phosphorylation of FAK in growth factor-stimulated cells. J Cell Sci 126, 745–55 (2013)

    230. Y. Tamura, Y. Takeuchi, M. Suzawa, S. Fukumoto, M. Kato, K. Miyazono, T. Fujita: Focal adhesion kinase activity is required for bone morphogenetic protein-Smad1 signaling and osteoblastic differentiation in murine MC3T3-E1 cells. J Bone Miner Res 16, 1772–9 (2001)

    231. Z. Mai, Z. Peng, S. Wu, J. Zhang, L. Chen, H. Liang, D. Bai, G. Yan, H. Ai: Single bout short duration fluid shear stress induces osteogenic differentiation of MC3T3-E1 cells via integrin beta1 and BMP2 signaling cross-talk. PLoS One 8, e61600 (2013)

    232. M.-P. Mena, I. Papiewska-Pajak, P. Przygodzka, A. Kozaczuk, J. Boncela, C.S. Cierniewski: NFAT2 regulates COX-2 expression and modulates the integrin repertoire in endothelial cells at the crossroads of angiogenesis and inflammation. Exp Cell Res 324, 124–36 (2014)

    233. M.E. Marquis, E. Lord, E. Bergeron, L. Bourgoin, N. Faucheux: Short-term effects of adhesion peptides on the responses of preosteoblasts to pBMP-9. Biomaterials 29, 1005–16 (2008)

    234. R.H. Li, J.M. Wozney: Delivering on the promise of bone morphogenetic proteins. Trends Biotechnol 19, 255–65 (2001)

    235. B.E. Rapuano, D.E. MacDonald: Structure-activity relationship of human bone sialoprotein peptides. Eur J Oral Sci 121, 600–9 (2013)

    236. L.A. Buchanan, A. El-Ghannam: Effect of bioactive glass crystallization on the conformation and bioactivity of adsorbed proteins. J Biomed Mater Res A 93, 537–46 (2010)

    237. A.B. Faia-Torres, T. Goren, T.O. Ihalainen, S. Guimond-Lischer, M. Charnley, M. Rottmar, K. Maniura-Weber, N.D. Spencer, R.L. Reis, M. Textor, N.M. Neves: Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: a gradient study. ACS Appl Mater Interfaces 7, 2367–75 (2015)

    238. A. Shekaran, A.J. Garcia: Extracellular matrix-mimetic adhesive biomaterials for bone repair. J Biomed Mater Res A 96, 261–72 (2011)

    239. J.H. Collier, T. Segura: Evolving the use of peptides as components of biomaterials. Biomaterials 32, 4198–204 (2011)

    240. O. Drevelle, E. Bergeron, H. Senta, M.A. Lauzon, S. Roux, G. Grenier, N. Faucheux: Effect of functionalized polycaprolactone on the behaviour of murine preosteoblasts. Biomaterials 31, 6468–76 (2010)

    241. Y.M. Shin, S.-Y. Jo, J.-S. Park, H.-J. Gwon, S.I. Jeong, Y.-M. Lim: Synergistic effect of dual-functionalized fibrous scaffold with BCP and RGD containing peptide for improved osteogenic differentiation. Macromol Biosci 14, 1190–8 (2014)

    242. M. Mehta, C.M. Madl, S. Lee, G.N. Duda, D.J. Mooney: The collagen I mimetic peptide DGEA enhances an osteogenic phenotype in mesenchymal stem cells when presented from cell-encapsulating hydrogels. J Biomed Mater Res A (2015)

    243. Y.M. Kolambkar, M. Bajin, A. Wojtowicz, D.W. Hutmacher, A.J. Garcia, R.E. Guldberg: Nanofiber orientation and surface functionalization modulate human mesenchymal stem cell behavior in vitro. Tissue Eng Part A 20, 398–409 (2014)

    244. A.M. Wojtowicz, A. Shekaran, M.E. Oest, K.M. Dupont, K.L. Templeman, D.W. Hutmacher, R.E. Guldberg, A.J. García: Coating of biomaterial scaffolds with the collagen-mimetic peptide GFOGER for bone defect repair. Biomaterials 31, 2574–82 (2010)

    245. B.A. Dalton, C.D. McFarland, P.A. Underwood, J.G. Steele: Role of the heparin binding domain of fibronectin in attachment and spreading of human bone-derived cells. J Cell Sci 108, 2083–92 (1995)

    246. S. Sun, W. Yu, Y. Zhang, F. Zhang: Increased preosteoblast adhesion and osteogenic gene expression on TiO2 nanotubes modified with KRSR. J Mater Sci Mater Med 24, 1079–91 (2013)

    247. K.C. Dee, T.T. Andersen, R. Bizios: Design and function of novel osteoblast-adhesive peptides for chemical modification of biomaterials. J Biomed Mater Res 40, 371–7 (1998)

    248. O. Drevelle, N. Faucheux: Biomimetic materials for controlling bone cell responses. Front Biosci (Schol Ed) 5, 369–95 (2013)

    249. A. Rezania, K.E. Healy: The effect of peptide surface density on mineralization of a matrix deposited by osteogenic cells. J Biomed Mater Res 52, 595–600 (2000)

    250. S.J. Bogdanowich-Knipp, S. Chakrabarti, T.D. Williams, R.K. Dillman, T.J. Siahaan: Solution stability of linear vs. cyclic RGD peptides. J Pept Res 53, 530–41 (1999)

    251. K.A. Kilian, M. Mrksich: Directing stem cell fate by controlling the affinity and density of ligand-receptor interactions at the biomaterials interface. Angew Chem Int Ed Engl 51, 4891–5 (2012)

    252. S.L. Bellis: Advantages of RGD peptides for directing cell association with biomaterials. Biomaterials 32, 4205–10 (2011)

    253. P.R. Patel, R.C. Kiser, Y.Y. Lu, E. Fong, W.C. Ho, D.A. Tirrell, R.H. Grubbs: Synthesis and cell adhesive properties of linear and cyclic RGD functionalized polynorbornene thin films. Biomacromolecules 13, 2546–53 (2012)

    254. L. Chen, C. Sloey, Z. Zhang, P. VBondarenko, H. Kim, D. Ren, S. Kanapuram: Chemical modifications of therapeutic proteins induced by residual ethylene oxide. J Pharm Sci 104, 731–9 (2015)

    255. N. Huebsch, M. Gilbert, K.E. Healy: Analysis of sterilization protocols for peptide-modified hydrogels. J Biomed Mater Res B Appl Biomater 74, 440–7 (2005)

    256. L.Y. Santiago, R.W. Nowak, J. Peter Rubin, K.G. Marra: Peptide-surface modification of poly(caprolactone) with laminin-derived sequences for adipose-derived stem cell applications. Biomaterials 27, 2962–9 (2006)

    257. S.-K. Min, H.K. Kang, D.H. Jang, S.Y. Jung, O.B. Kim, B.-M. Min, I.-S. Yeo: Titanium surface coating with a laminin-derived functional peptide promotes bone cell adhesion. Biomed Res Int 2013, 638348 (2013)

    258. H.K. Kang, O.B. Kim, S.-K. Min, S.Y. Jung, D.H. Jang, T.-K. Kwon, B.-M. Min, I.-S. Yeo: The effect of the DLTIDDSYWYRI motif of the human laminin alpha2 chain on implant osseointegration. Biomaterials 34, 4027–37 (2013)

    259. Y.J. Choi, J.Y. Lee, S.J. Lee, C.-P. Chung, Y.J. Park: Determination of osteogenic or adipogenic lineages in muscle-derived stem cells (MDSCs) by a collagen-binding peptide (CBP) derived from bone sialoprotein (BSP). Biochem Biophys Res Commun 419, 326–32 (2012)

    260. X. Chen, P. Sevilla, C. Aparicio: Surface biofunctionalization by covalent co-immobilization of oligopeptides. Colloids Surf B Biointerfaces 107, 189–97 (2013)

    261. K. Oya, Y. Tanaka, H. Saito, K. Kurashima, K. Nogi, H. Tsutsumi, Y. Tsutsumi, H. Doi, N. Nomura, T. Hanawa: Calcification by MC3T3-E1 cells on RGD peptide immobilized on titanium through electrodeposited PEG. Biomaterials 30, 1281–6 (2009)

    262. R.N. Palchesko, J.D. Romeo, K.A. McGowan, E.S. Gawalt: Increased osteoblast adhesion on physically optimized KRSR modified calcium aluminate. J Biomed Mater Res Part A 100, 1229–38 (2012)

    263. A.A. Sawyer, K.M. Hennessy, S.L. Bellis: The effect of adsorbed serum proteins, RGD and proteoglycan-binding peptides on the adhesion of mesenchymal stem cells to hydroxyapatite. Biomaterials 28, 383–92 (2007)

    264. J. Kim, C. Ki, Y. Park, H. Kim, I. Um: Effect of RGDS and KRSR peptides immobilized on silk fibroin nanofibrous mats for cell adhesion and proliferation. Macromol Res 18, 442–8 (2010)

    265. N. Broggini, S. Tosatti, S.J. Ferguson, M. Schuler, M. Textor, M.M. Bornstein, D.D. Bosshardt, D. Buser: Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)-and heparin (KRSR)-binding peptides. J Biomed Mater Res A 100, 703–11 (2012)

    266. S. Aota, M. Nomizu, K.M. Yamada: The short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function. J Biol Chem 269, 24756–61 (1994)

    267. R. Nakaoka, Y. Hirano, D.J. Mooney, T. Tsuchiya, A. Matsuoka: Study on the potential of RGD-and PHSRN-modified alginates as artificial extracellular matrices for engineering bone. J Artif Organs 16, 284–93 (2013)

    268. D.S.W. Benoit, K.S. Anseth: The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces. Biomaterials 26, 5209–20 (2005)

    269. C.D. Reyes, T.A. Petrie, A.J. Garcia: Mixed extracellular matrix ligands synergistically modulate integrin adhesion and signaling. J Cell Physiol 217, 450–8 (2008)

    270. A.J. Garcia, J.E. Schwarzbauer, D. Boettiger: Distinct activation states of alpha5beta1 integrin show differential binding to RGD and synergy domains of fibronectin. Biochemistry 41, 9063–9 (2002)

    271. R. Visser, P.M. Arrabal, L. Santos-Ruiz, R. Fernandez-Barranco, J. Becerra, M. Cifuentes: A collagen-targeted biomimetic RGD peptide to promote osteogenesis. Tissue Eng Part A 20, 34–44 (2014)

    272. B. Han, F.L. Hall, M.E. Nimni: Refolding of a recombinant collagen-targeted TGF-beta2 fusion protein expressed in Escherichia coli. Protein Expr Purif 11, 169–78 (1997)

    273. A. Shekaran, J.R. Garcia, A.Y. Clark, T.E. Kavanaugh, A.S. Lin, R.E. Guldberg, A.J. Garcia: Bone regeneration using an alpha 2 beta 1 integrin-specific hydrogel as a BMP-2 delivery vehicle. Biomaterials 35, 5453–61 (2014)

    274. M.A. Brown, Q. Zhao, K.A. Baker, C. Naik, C. Chen, L. Pukac, M. Singh, T. Tsareva, Y. Parice, A. Mahoney, V. Roschke, I. Sanyal, S. Choe: Crystal structure of BMP-9 and functional interactions with pro-region and receptors. J Biol Chem 280, 25111–8 (2005)

    275. Y. Suzuki, M. Tanihara, K. Suzuki, A. Saitou, W. Sufan, Y. Nishimura: Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo. J Biomed Mater Res 50, 405–9 (2000)

    276. A. Saito, Y. Suzuki, S. Ogata, C. Ohtsuki, M. Tanihara: Activation of osteo-progenitor cells by a novel synthetic peptide derived from the bone morphogenetic protein-2 knuckle epitope. Biochim Biophys Acta 1651, 60–7 (2003)

    277. E. Bergeron, H. Senta, A. Mailloux, H. Park, E. Lord, N. Faucheux: Murine preosteoblast differentiation induced by a peptide derived from bone morphogenetic proteins-9. Tissue Eng A 15, 3341–9 (2009)

    278. E. Bergeron, E. Leblanc, O. Drevelle, R. Giguere, S. Beauvais, G. Grenier, N. Faucheux: The evaluation of ectopic bone formation induced by delivery systems for bone morphogenetic protein-9 or its derived peptide. Tissue Eng A 18, 342–52 (2012)

    279. N.M. Moore, N.J. Lin, N.D. Gallant, M.L. Becker: Synergistic enhancement of human bone marrow stromal cell proliferation and osteogenic differentiation on BMP-2-derived and RGD peptide concentration gradients. Acta Biomater 7, 2091–100 (2011)

    280. O.F. Zouani, C. Chollet, B. Guillotin, M.-C. Durrieu: Differentiation of pre-osteoblast cells on poly(ethylene terephthalate) grafted with RGD and/or BMPs mimetic peptides. Biomaterials 31, 8245–53 (2010)

    281. W.-N. Yin, F.-Y. Cao, K. Han, X. Zeng, R.-X. Zhuo, X.-Z. Zhang: The synergistic effect of a BMP-7 derived peptide and cyclic RGD in regulating differentiation behaviours of mesenchymal stem cells. J Mater Chem B 2, 8434–40 (2014)

    282. C.M. Rubert Perez, N. Stephanopoulos, S. Sur, S.S. Lee, C. Newcomb, S.I. Stupp: The powerful functions of peptide-based bioactive matrices for regenerative medicine. Ann Biomed Eng 43, 501–14 (2015)

    283. S.S. Lee, E.L. Hsu, M. Mendoza, J. Ghodasra, M.S. Nickoli, A. Ashtekar, M. Polavarapu, J. Babu, R.M. Riaz, J.D. Nicolas, D. Nelson, S.Z. Hashmi, S.R. Kaltz, J.S. Earhart, B.R. Merk, J.S. McKee, S.F. Bairstow, R.N. Shah, W.K. Hsu, S.I. Stupp: Gel scaffolds of BMP-2-binding peptide amphiphile nanofibers for spinal arthrodesis. Adv Healthc Mater 4, 131–41 (2015)

    284. Y. Song, Y. Ju, Y. Morita, B. Xu, G. Song: Surface functionalization of nanoporous alumina with bone morphogenetic protein 2 for inducing osteogenic differentiation of mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl 37, 120–6 (2014)

    285. F. Haasters, D. Docheva, C. Gassner, C. Popov, W. Bocker, W. Mutschler, M. Schieker, W.C. Prall: Mesenchymal stem cells from osteoporotic patients reveal reduced migration and invasion upon stimulation with BMP-2 or BMP-7. Biochem Biophys Res Commun 452, 118–23 (2014)

    286. M. Kisiel, M.M. Martino, M. Ventura, J.A. Hubbell, J. Hilborn, D.A. Ossipov: Improving the osteogenic potential of BMP-2 with hyaluronic acid hydrogel modified with integrin-specific fibronectin fragment. Biomaterials 34, 704–12 (2013)

    287. Z. Hamidouche, O. Fromigue, J. Ringe, T. Haupl, P.J. Marie: Crosstalks between integrin alpha 5 and IGF2/IGFBP2 signalling trigger human bone marrow-derived mesenchymal stromal osteogenic differentiation. BMC Cell Biol 11, 44 (2010)

    288. G.H. Mahabeleshwar, W. Feng, K. Reddy, E.F. Plow, T. V Byzova: Mechanisms of integrin-vascular endothelial growth factor receptor cross-activation in angiogenesis. Circ Res 101, 570–80 (2007)

    289. S. Mori, C.-Y. Wu, S. Yamaji, J. Saegusa, B. Shi, Z. Ma, Y. Kuwabara, K.S. Lam, R.R. Isseroff, Y.K. Takada, Y. Takada: Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. J Biol Chem 283, 18066–75 (2008)

    290. S. Yamaji, J. Saegusa, K. Ieguchi, M. Fujita, S. Mori, Y.K. Takada, Y. Takada: A novel fibroblast growth factor-1 (FGF1) mutant that acts as an FGF antagonist. PLoS One 5, e10273 (2010)

    291. Z. Dai, F. Guo, F. Wu, H. Xu, C. Yang, J. Li, P. Liang, H. Zhang, L. Qu, Y. Tan, Y. Wan, Y. Li: Integrin alphavbeta3 mediates the synergetic regulation of core-binding factor alpha1 transcriptional activity by gravity and insulin-like growth factor-1 through phosphoinositide 3-kinase signaling. Bone 69, 126–32 (2014)

    292. J.-L. Su, J. Chiou, C.-H. Tang, M. Zhao, C.-H. Tsai, P.-S. Chen, Y.-W. Chang, M.-H. Chien, C.-Y. Peng, M. Hsiao, M.-L. Kuo, M.-L. Yen: CYR61 regulates BMP-2-dependent osteoblast differentiation through the {alpha}v{beta}3 integrin/integrin-linked kinase/ERK pathway. J Biol Chem 285, 31325–36 (2010)

Share and Cite
Sabrina Beauvais, Olivier Drevelle, Jessica Jann, Marc-Antoine Lauzon, Mohammadreza Foruzanmehr, Guillaume Grenier, Sophie Roux, Nathalie Faucheux. Interactions between bone cells and biomaterials: An update. Frontiers in Bioscience-Scholar. 2016. 8(2); 227-263.