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Menstrual endometrial supernatant may induce stromal endometriosis in baboons
Cleophas Mutinda Kyama1,2,3,5,Henrik Falconer2,4,Silvio Cuneo2,Daniel Chai2,Attila Mihalyi1,3,Jason Mwenda2,Thomas D’Hooghe2,3,*
1
Experimental Laboratory for Gynaecology, Department of Obstetrics & Gynaecology, University Hospital Gasthuisberg, Herestraat 49, B3000 Leuven, Belgium
2
Division of Reproductive Health and Biology, Institute of Primate Research, P.O BOX 24481, End of Karen Road, 00502 Karen, Nairobi, Kenya
3
Leuven University Fertility Centre, Department of Obstetrics & Gynaecology, University Hospital Gasthuisberg, Herestraat 49, B3000 Leuven, Belgium
4
Division for Obstetrics and Gynaecology, Department of Woman and Child Health, Karolinska Institute, Stockholm, Sweden
5
Department of Medical Laboratory Sciences, Institute of Tropical Medicine and Infectious Diseases, Jomo Kenyatta University of Agriculture and Technology, Kenya
DOI: 10.2741/S410 Volume 6 Issue 1, pp.16-28
Published: 01 January 2014
(This article belongs to the Special Issue Frontiers in endometriosis)
*Corresponding Author(s):  
Thomas D’Hooghe
E-mail:  
thomas.dhooghe@uzleuven.be
Abstract

We tested the hypothesis that endometriosis can be induced in baboons more successfully by intra-pelvic injection of the pellet of menstrual endometrium when compared to its supernatant. Menstrual endometrium, separated into pellet (n = 5) and supernatant fractions ( n = 8), or phosphate buffered saline (1 ml, n = 7, controls) was injected laparoscopically into the pelvis. During laparoscopy 25 days later, the number (ρ = 0.027) and surface area (ρ <0.0001) of endometriosis-like lesions were significantly higher in the pellet group, than in the supernatant group, or in the control group. Histological typical endometriosis was present only in the endometrial pellet group (1/15), whereas stromal endometriosis was observed in both the pellet group (6/15), and the supernatant group (6/20). Peritoneal endometrial like glands were observed in both the endometrial pellet group (3/15), and in the supernatant group (1/20). In conclusion, we confirmed our hypothesis that endometriosis can be induced in baboons more successfully by intrapelvic injection of the pellet of menstrual endometrium when compared to its supernatant.

Key words

Typical Endometriosis, Stromal Endometriosis, Menstrual Endometrial Supernatant, Menstrual Endometrial Pellet, Baboons; White And Red Lesions

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Cleophas Mutinda Kyama, Henrik Falconer, Silvio Cuneo, Daniel Chai, Attila Mihalyi, Jason Mwenda, Thomas D’Hooghe. Menstrual endometrial supernatant may induce stromal endometriosis in baboons. Frontiers in Bioscience-Scholar. 2014. 6(1); 16-28.