Open Access
Article

Initiation of oogenesis and meiosis in the fetal ovary depends on Dennd1a-mediated production of Wnt5a and retinoic acid from the somatic niches

Jingjing Shi1,2,3,4,Qun Niu1,2,3,4,Qing Gao5,Jiang Fu1,2,3,4,*,,Jinlong Ma1,2,3,4,*
1
Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, 250012 Jinan, Shandong, China
2
Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, 250012 Jinan, Shandong, China
3
Shandong Key Laboratory of Reproductive Medicine, Shandong University, 250012 Jinan, Shandong, China
4
Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, 250012 Jinan, Shandong, China
5
Department of Histology and Embryology, School of Basic Medicine, Cheeloo College of Medicine, Shandong University, 250012 Jinan, Shandong, China
DOI: 10.52586/5045 Volume 26 Issue 12, pp.1513-1524
Submited: 01 September 2021 Revised: 01 November 2021
Accepted: 05 November 2021 Published: 30 December 2021
*Corresponding Author(s):  
Jiang Fu
E-mail:  
fujiang@sdu.edu.cn
*Corresponding Author(s):  
Jinlong Ma
E-mail:  
majinlong@sduivf.com
Copyright: © 2021 The author(s). Published by BRI. This is an open access article under the CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/).
Abstract

Background: The DENN (differentially expressed in neoplastic versus normal cells) domain containing 1A (Dennd1a), a guanine nucleotide exchange factor (GEF) for the small GTPase Rab35, is essential for mouse embryogenesis. Disruption of Dennd1a impairs the migration and differentiation of fetal germ cells. In the present study, we further elucidated the role of Dennd1a in oogenesis and meiosis in the fetal ovary. Results: Ablation of Dennd1a disrupted the mRNA expression of Sohlh2, Figla, Stra8, and Rec8 in the ovary of Dennd1a-/- mutants at E13.5. Using ex vivo culture of E12.5 female gonads and adenoviral Dennd1a shRNA infection, we demonstrated that transcription of Sohlh2, Figla, Stra8 and Rec8 were not activated in the fetal ovary lacking Dennd1a. Dennd1a in the somatic cells might stimulate Sohlh2 expression at early stage of oocyte differentiation via regulating Wnt5a synthesis. On the other hand, meiotic initiation of the fetal germ cells required Dennd1a-mediated RA production from the somatic cells, which induced the expression of Stra8 and Rec8. Conclusions: Dennd1a could be involved in multiple signal pathways in the somatic cells that are critical for various processes of oogenesis and meiosis in the fetal ovary.

Key words

Dennd1a; Wnt5a; Retinoic acid; Fetal oogenesis; Meiosis

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Jingjing Shi, Qun Niu, Qing Gao, Jiang Fu, Jinlong Ma. Initiation of oogenesis and meiosis in the fetal ovary depends on Dennd1a-mediated production of Wnt5a and retinoic acid from the somatic niches. Frontiers in Bioscience-Landmark. 2021. 26(12); 1513-1524.