Open Access
Article
Retroviral recombination: review of genetic analyses
Wei-Shau Hu1,Terence Rhodes1,Que Dang1,Vinay Pathak1
1
HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA. whu@ncifcrf.gov
DOI: 10.2741/940 Volume 8 Issue 4, pp.143-155
Published: 01 January 2003
(This article belongs to the Special Issue Engineered retroviruses)
Abstract

Retroviruses package two copies of genomic RNA into one virion. One of the essential steps of replication is reverse transcription, in which the virally encoded enzyme reverse transcriptase (RT) uses the packaged RNA as a template to synthesize viral DNA. Because two copies of RNA are present in one virion, it is possible for RT to switch from one copy of the viral RNA to the other copy during DNA synthesis, thereby generating a recombinant containing some genetic information from each of the RNAs. Recombination occurs at high frequencies during retroviral replication. This frequent recombination has a significant impact on the current human immunodeficiency virus type 1 (HIV-1) epidemic as well as the development of retrovirus-based systems for gene therapy. In this review, the rates, mechanisms, and properties of retroviral recombination are summarized from recent genetic studies. Implications of these studies are also discussed.

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Wei-Shau Hu, Terence Rhodes, Que Dang, Vinay Pathak. Retroviral recombination: review of genetic analyses. Frontiers in Bioscience-Landmark. 2003. 8(4); 143-155.