Open Access
Article
Isoflurane attenuates LPS-induced acute lung injury by targeting miR-155-HIF1-alpha
Rong Hu1,Ying Zhang1,Xiaohua Yang2,Jia Yan1,Yu Sun1,Zhifeng Chen1,Hong Jiang1,*
1
Department of Anesthesiology, Shanghai Ninth People’s Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, No. 639, Zhi Zao Ju Road, Shanghai, China
2
Central Laboratory, Shanghai Chest Hospital affiliated to Shanghai Jiao Tong University, Shanghai, China
DOI: 10.2741/4302 Volume 20 Issue 1, pp.139-156
Published: 01 January 2015
(This article belongs to the Special Issue Cellular immunology and stem cell biology)
*Corresponding Author(s):  
Hong Jiang
E-mail:  
dr_jianghong@163.com
Abstract

Isoflurane alleviates the inflammatory response in endotoxin-induced acute lung injury (ALI). In this study, we investigated the protective mechanism of isoflurane postconditioning in lipopolysaccharide (LPS)-induced ALI. Exposure to isoflurane decreased miR-155 and upregulated HIF-1 alpha and HO-1 mRNA and protein. The effects of isoflurane on HIF-1 alpha mRNA and protein could be inhibited by overexpression of miR-155. Furthermore, mice overexpressing miR-155 had higher levels of TNF-alpha and IL-1 beta in BALF when exposed to isoflurane after LPS challenge. Conversely, downregulation of miR-155 promoted isoflurane effects on HIF-1 alpha expression. These results suggest that isoflurane posttreatment at 1 MAC for 4 hour alleviates LPS-induced ALI and cell injury by triggering miR-155-HIF-1 alpha pathway, leading to upregulation of HO-1.

Key words
Isoflurane, Posttreatment, Endotoxin, Acute Lung Injury, miR-155, HIF1 alpha
Share and Cite
Rong Hu, Ying Zhang, Xiaohua Yang, Jia Yan, Yu Sun, Zhifeng Chen, Hong Jiang. Isoflurane attenuates LPS-induced acute lung injury by targeting miR-155-HIF1-alpha. Frontiers in Bioscience-Landmark. 2015. 20(1); 139-156.