Open Access
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Inhibition of macrophage autophagy induced by Salmonella enterica serovar typhi plasmid

ShuYan Wu1,YuanYuan Chu1,YanRu Yang1,YuanYuan Li1,PeiYan He1,YaJie Zheng1,Chi Zhang1,QiuChen Liu1,Ling Han1,Rui Huang1,*
1
Medical College of Soochow University, No. 199, Ren Ai Road, Suzhou, Jiangsu 215123, P. R. China
DOI: 10.2741/4220 Volume 19 Issue 3, pp.490-503
Published: 01 January 2014
(This article belongs to the Special Issue Autophagy: biology and disease)
*Corresponding Author(s):  
Rui Huang
E-mail:  
hruisdm@163.com
Abstract

pRST98, a chimeric plasmid isolated from Salmonella enterica serovar typhi (S. typhi), is involved in bacterial multidrug-resistance and virulence, however, its exact contributions to bacterial pathogenesis are still not fully understood. To investigate whether pRST98 exhibits potential to mediate macrophage autophagy and apoptosis, murine macrophage-like cell line (J774A.1) was infected with wild type strain (S. typhi-WT), mutant strain (S. typhi-DeltapRST98) and complement of S. typhi-DeltapRST98 (S. typhi-c-pRST98). Results revealed that S. typhi harboring pRST98 decreased the number of autophagy vacuoles of macrophages as well as the expression of Beclin 1 and LC3-II at the early stage of infection; apoptosis rate of macrophages infected with S. typhi-DeltapRST98 was lower than that infected with S. typhi-WT or S. typhi-c-pRST98. The survival rate of intracellular bacteria carrying pRST98 was much higher than that of plasmid free strain. After intervention with autophagy agonist rapamycin, apoptosis rate of the cells infected with S. typhi containing pRST98 and intracellular bacterial growth decreased. Our study suggested that pRST98 could inhibit autophagy and induce cell apoptosis for the host bacterial survival and proliferation.

Key words

Salmonella, Plasmid, Autophagy, Apoptosis, Macrophage

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ShuYan Wu, YuanYuan Chu, YanRu Yang, YuanYuan Li, PeiYan He, YaJie Zheng, Chi Zhang, QiuChen Liu, Ling Han, Rui Huang. Inhibition of macrophage autophagy induced by Salmonella enterica serovar typhi plasmid. Frontiers in Bioscience-Landmark. 2014. 19(3); 490-503.