Open Access
AlphaVBeta5 integrins mediates Pseudomonas fluorescens interaction with A549 cells
Elisabetta Buommino1,Marina Di Domenico2,*,Iole Paoletti1,Alessandra Fusco1,Vincenza De Gregorio1,Valentina Cozza3,Antonietta Rizzo1,Maria Antonietta Tufano1
Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Italy
Department of General Pathology, Faculty of Medicine of Naples, Second University of Naples, Naples, Italy
Department of business management, University of Naples Parthenope, Naples, Italy
DOI: 10.2741/4215 Volume 19 Issue 3, pp.408-415
Published: 01 January 2014
(This article belongs to the Special Issue Gene targets for modulating cell growth)
*Corresponding Author(s):  
Marina Di Domenico

Interaction of pathogenic bacteria with human cells is usually an essential step in the infection process. The bacterial invasion is stimulated by microbial binding to mammalian extracellular matrix proteins such as vitronectin, fibronectin or integrins. We have recently shown that some strains isolated from a clinical environment are able to grow at/or above 37°C. In particular, we demonstrated that P. fluorescens AF181 binds specifically to the surface of A549 human respiratory epithelial cells and that adhesiveness modulates the inflammatory response. In this study, the involvement of Alpha(v)Beta5 integrins and its respective natural ligand vitronectin (VN) in P. fluorescens AF181 adherence and invasion was examined. The host cell cytoskeleton and cellular tyrosine kinases seem to be solicited during the P. fluorescens-respiratory cell interaction; consequently, cytochalasin D and genistein decreased the bacterial adherence and internalization. Gene silencing of α(v), β5 integrins and vitronectin reduced P. fluorescens adherence and internalization to A549 cells. Taken together, these findings suggest that Alpha(v)Beta5 integrins and their natural ligand VN are involved in P. fluorescens adherence and invasion in human epithelial cells.

Key words

Pseudomonas fluorescens, Integrins, Vitronectin

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Elisabetta Buommino, Marina Di Domenico, Iole Paoletti, Alessandra Fusco, Vincenza De Gregorio, Valentina Cozza, Antonietta Rizzo, Maria Antonietta Tufano. AlphaVBeta5 integrins mediates Pseudomonas fluorescens interaction with A549 cells. Frontiers in Bioscience-Landmark. 2014. 19(3); 408-415.