Open Access
Article

Bactericidal activity of starch-encapsulated gold nanoparticles

Dillon S Pender1,Lakshmisri M Vangala1,Vivek D Badwaik1,Chad B Willis1,Zoraida P Aguilar2,Tejas N Sangoi3,Rammohan Paripelly1,Rajalingam Dakshinamurthy1,*
1
Department of Chemistry, Western Kentucky University, Bowling Green, KY 42101, USA
2
Ocean Nano Tech, Springdale, AR 72764, USA
3
The Carol Martin Gatton Academy of Mathematics and Science, Western Kentucky University, Bowling Green, KY 42101, USA
DOI: 10.2741/4158 Volume 18 Issue 3, pp.993-1002
Published: 01 June 2013
(This article belongs to the Special Issue Nano and bio sensors)
*Corresponding Author(s):  
Rajalingam Dakshinamurthy
E-mail:  
rajalingam.dakshinamurthy@wku.edu
Abstract

We report the bactericidal applications of ecofriendly starch encapsulated gold nanoparticles (StAuNPs). The mechanism of interaction of the properly characterized St-AuNPs with both Gram negative and Gram positive bacteria were investigated using spread plate assay, transmission electron microscopy (TEM), and fluorescent propidium iodide (PI) exclusion assay. The StAuNPs were found to possess significant dose dependent antibacterial activity against both types of bacteria. StAuNPs at 1.2 mg/mL caused 98 % eradication of Gram positive bacteria that was exposed over a period of 12 h. Similarly, 4.8 mg/mL St-AuNPs caused 98 % eradication of Gram negative bacteria over a period of 12h. The StAuNPs are biocompatible and present a useful solid porous carbohydrate-based polymer vehicle with excellent antimicrobial activity against both Gram negative and positive bacteria.

Key words

Starch, gold nanoparticles, multifunctional gold nanoparticles, antibacterial activity

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Dillon S Pender, Lakshmisri M Vangala, Vivek D Badwaik, Chad B Willis, Zoraida P Aguilar, Tejas N Sangoi, Rammohan Paripelly, Rajalingam Dakshinamurthy. Bactericidal activity of starch-encapsulated gold nanoparticles. Frontiers in Bioscience-Landmark. 2013. 18(3); 993-1002.