Open Access
Article

Hematopoietic stem cells improve dopaminergic neuron in the MPTP-mice

Jingwei Lu1,Suman Kanji1,Reeva Aggarwal1,Manjusri Das1,Matthew Joseph1,Lai-Chu Wu2,Hai-Quan Mao3,Vincent J. Pompili1,Maria Hadjiconstantinou4,Hiranmoy Das1,*
1
Stem Cell Research Laboratory, Cardiovascular Medicine, Davis Heart and Lung Research Institute, Wexner Medical Center at The Ohio State University, Columbus, Ohio 43210, USA
2
Molecular and Cellular Biochemistry, College of Medicine, Wexner Medical Center at The Ohio State University, Columbus, Ohio, USA
3
Department of Materials Science and Engineering, John’s Hopkins University, Baltimore, MD, USA
4
Departments of Psychiatry and Pharmacology, Division of Molecular Psychopharmacology, Wexner Medical Center at The Ohio State University, Columbus, Ohio, USA
DOI: 10.2741/4156 Volume 18 Issue 3, pp.970-981
Published: 01 June 2013
*Corresponding Author(s):  
Hiranmoy Das
E-mail:  
hiranmoy.das@osumc.edu
Abstract

Because of their ability for self-renewal and neural differentiation, stem cells are believed to be ideal for cell replacement therapy in Parkinson’s disease (PD). Nanofiber-expanded human umbilical cord hematopoietic stem cells (HUHSCs) are advantageous to other stem cells as they provide a source of unlimited stem cell production for clinical application. In this study, we investigated whether 1. nanofiber-expanded HUHSCs are capable of neural differentiation in vitro, and 2. they could improve dopaminergic neuron morphology in the caudate/putamen (CPu) and substantia nigra pars compacta (SNc) of the MPTP-mouse model of PD. When cultured under neural differentiation conditions, nanofiber-expanded HUHSCs were able to undergo neural differentiation in vitro, as determined by gene and protein expression of neural markers such as MAP2, NeuN, HuC, GFAP and Oligo2. Thirty days after a single intracardioventricular injection of HUHSCs to MPTP-mice there was a significant recovery of tyrosine hydroxylase (TH) immunostaining in CPu. There was an increase in the size and staining density of TH+ cells in SNc, while their number was unchanged.

Key words

Umbilical Cord. Stem Cells, Parkinson’s Disease, Neuron, Regeneration

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Jingwei Lu, Suman Kanji, Reeva Aggarwal, Manjusri Das, Matthew Joseph, Lai-Chu Wu, Hai-Quan Mao, Vincent J. Pompili, Maria Hadjiconstantinou, Hiranmoy Das. Hematopoietic stem cells improve dopaminergic neuron in the MPTP-mice. Frontiers in Bioscience-Landmark. 2013. 18(3); 970-981.