Open Access
Review
A proposal: Source of single strand DNA that elicits the SOS response
Chiara Indiani1,*,Mike O’Donnell2
1
Chiara Indiani Manhattan College 4513 Manhattan College Pkwy, Riverdale, NY 10471, USA
2
Mike O’Donnell The Rockefeller University Howard Hughes Medical Institute, 1230 York Avenue New York, NY 10065, USA
DOI: 10.2741/4102 Volume 18 Issue 1, pp.312-323
Published: 01 January 2013
(This article belongs to the Special Issue Replication in the presence of DNA damage)
*Corresponding Author(s):  
Chiara Indiani
E-mail:  
chiara.indiani@manhattan.edu
Abstract

Chromosome replication is performed by numerous proteins that function together as a “replisome”. The replisome machinery duplicates both strands of the parental DNA simultaneously. Upon DNA damage to the cell, replisome action produces single-strand DNA to which RecA binds, enabling its activity in cleaving the LexA repressor and thus inducing the SOS response. How singlestrand DNA is produced by a replisome acting on damaged DNA is not clear. For many years it has been assumed the single-strand DNA is generated by the replicative helicase, which continues unwinding DNA even after DNA polymerase stalls at a template lesion. Recent studies indicate another source of the single-strand DNA, resulting from an inherently dynamic replisome that may hop over template lesions on both leading and lagging strands, thereby leaving single-strand gaps in the wake of the replication fork. These single-strand gaps are proposed to be the origin of the single-strand DNA that triggers the SOS response after DNA damage.

Key words

SOS response, Translesion Synthesis, DNA repair, lesion bypass, DNA polymerase, Sliding Clamp, Review

Share and Cite
Chiara Indiani, Mike O’Donnell. A proposal: Source of single strand DNA that elicits the SOS response. Frontiers in Bioscience-Landmark. 2013. 18(1); 312-323.