Open Access
SNCG gene silencing in gallbladder cancer cells inhibits key tumorigenic activities
Shenghua Han1,Feifei She1,Dong Wang1,Xiangqing Yao1,Lei Jiang1,Yanling Chen1
Department of Hepatobiliary Surgery, Union Hospital, Fujian Medical University, Fuzhou 350001, China
DOI: 10.2741/4005 Volume 17 Issue 4, pp.1589-1598
Published: 01 January 2012
(This article belongs to the Special Issue Pathogenesis and diagnostic modalities in cancer)

We recently determined that synuclein-gamma (SNCG) is highly expressed in human gallbladder cancer (GBC), and its abnormal expression is associated with tumor aggressiveness. To investigate the effects of SNCG gene silencing on the tumorigenic profiles of the GBC cell line, NOZ, short-hairpin RNA (shRNA) interference was employed. Specifically, the SNCG transcript was targeted by SNCG-shRNA lentiviral particles designed to silence SNCG gene expression. Following selection of NOZ cells stably expressing SNCG-shRNA, SNCG expression was examined by western blot and semi-quantitative RT-PCR analyses. Phenotypic hallmarks of gallbladder carcinogenesis were assayed by CCK-8, soft agar (colony formation), modified Boyden-Chamber (invasion), and flow cytometry (cell-cycle and apoptosis) assays. Our results showed that SNCG gene silencing in NOZ cells inhibited cell growth, colony formation, and invasion. In addition, it directly increased the effectiveness of paclitaxel in inducing G2/M cell-cycle arrest and cell apoptosis. Data from our in vivo study showed a decrease in tumor growth and weight in mice injected with SNCG-silenced NOZ cells. Together, these findings suggest that SNCG plays an important role in the progression of GBC.

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Shenghua Han, Feifei She, Dong Wang, Xiangqing Yao, Lei Jiang, Yanling Chen. SNCG gene silencing in gallbladder cancer cells inhibits key tumorigenic activities. Frontiers in Bioscience-Landmark. 2012. 17(4); 1589-1598.