Open Access
Article
Thymosin-alpha1 promotes the apoptosis of regulatory T cells and survival rate in septic mice
Jian Wan1,Yi Shan1,Hongwei Shan1,Guomin Li1,Tao Wang1,Jun Guan1,Xuefeng Liu1,Dechang Chen1,Wenfang Li1,Zhaofen Lin1
1
Department of emergency and critical care medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, 200003, China
DOI: 10.2741/3894 Volume 16 Issue 8, pp.3004-3013
Published: 01 June 2011
(This article belongs to the Special Issue Immunoregulation and inflammatory disease)
Abstract

Tregs are involved in immune disorder during sepsis; they can lead to a Th2 immune reaction. Their inhibitory effects can help alleviate inflammatory injury, but may also cause secondary immune inhibition. Thymosin-alpha1 is a polypeptide with powerful immunomodulatory activities. Current reports have shown that Thymosin-alpha1 conferred beneficial effects to septic patients. To explore the relationship between Thymosin-alpha1 and Tregs, in this study, we investigated the changing trend in CD4(+)CD25(+)Foxp3(+) T lymphocytes in a CLP septic mouse model. We also investigated the variation of apoptotic rate of CD4(+)CD25(+) T lymphocytes, cytokine variation, and change of model survival rate when Thymosin-alpha1 intervening or not. We observed that the 72-h survival rate was improved, the percentage of CD4(+)CD25(+)Foxp3(+) T lymphocytes decreased and the apoptosis rate of CD4(+)CD25(+) T lymphocytes increased after intervention of Thymosin-alpha1. At same time the expression of pro-inflammation cytokines IL-2, TNF-alpha and anti-inflammatory cytokines IL-10 and TGF-beta were regulated. In conclusion, Thymosin-alpha1 can effectively control the inflammatory response intensity and improve the 72-h survival rate of septic mice. Regulating Tregs may be another important role of Thymosin-alpha1 conditioning the immune reaction in sepsis.

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Jian Wan, Yi Shan, Hongwei Shan, Guomin Li, Tao Wang, Jun Guan, Xuefeng Liu, Dechang Chen, Wenfang Li, Zhaofen Lin. Thymosin-alpha1 promotes the apoptosis of regulatory T cells and survival rate in septic mice. Frontiers in Bioscience-Landmark. 2011. 16(8); 3004-3013.