Open Access
Experimental study of 99mTc-depreotide preparation and its affinity with A549 cell
Wei Xia1,Xiaoping Zhang1,Fei Yu1,Peijun Wang1,Haidong Cai1,Renhua Hou1,Guoyu Wang1,Xueyu Yuan1,Zhongwei LV1
Department of Nuclear Medicine, 10th People's Hospital, Tongji University of Medcine School, Shanghai 200072, China
DOI: 10.2741/3870 Volume 16 Issue 7, pp.2527-2539
Published: 01 June 2011
(This article belongs to the Special Issue Pathogenesis and diagnostic modalities in cancer)

The (99m)Tc-labeled agent, ((99m)TcO)depreotide, has received regulatory approval in the United States and Europe for use in the detection of cancer. It is essential to establish a simple and reliable method of direct radiolabeling of (99m)Tc-depreotide and to investigate its specific receptor binding properties with human non small cell lung cancer (NSCLC) A549 cell in vitro. So we made some researches as follow: Depreotide was labeled with (99m)Tc using SnCl2 as a reductant. Labeling efficiencies at different pH values and temperatures were compared. Radioreceptor assay was used to observe the uptake kinetics, stagnation and retention half time of (99m)Tc-depreotide in A549 cells. As the results of the investigation ,many facts is shown below: The labeling rate of pH 6.0 group was higher than that of pH 5.0 and pH7.0 groups. The labeling rate decreased when temperature increased from 15 °C to 50 °C. The uptake rate increased with rising temperature, and the maximum uptake was observed at 60 min at 37 °C. The cleaning curves were similar at different temperatures, and the half cleaning time at 37 °C was 48 min. The results showed that the optimal conditions for labeling depreotide with (99m)Tc was found to be below 15 °C at a pH lower than 6.0. Furthermore, at 37 °C, (99m)Tc-depreotid may have the potential as an ideal imaging agent for somatostatin receptors.

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Wei Xia, Xiaoping Zhang, Fei Yu, Peijun Wang, Haidong Cai, Renhua Hou, Guoyu Wang, Xueyu Yuan, Zhongwei LV. Experimental study of 99mTc-depreotide preparation and its affinity with A549 cell. Frontiers in Bioscience-Landmark. 2011. 16(7); 2527-2539.