Open Access
Article
Functional characterization of MIMP for its adhesion to the intestinal epithelium
Zhihua Liu1,Tongyi Shen1,Hongqi Chen1,Yukun Zhou1,Peng Zhang1,Yanlei Ma1,Mary Pat Moyer1,Ming Zhang1,Zhaoxin Chu1,Huanlong Qin1
1
Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China
DOI: 10.2741/3842 Volume 16 Issue 6, pp.2106-2127
Published: 01 June 2011
(This article belongs to the Special Issue Immunoregulation and inflammatory disease)
Abstract

The micro integral membrane protein (MIMP), the domain within the integral membrane protein of Lactobacillus plantarum CGMCC 1258, has been shown to adhere to mucin and antagonize the adhesion of enteroinvasive E. coli and enteropathogenic E. coli. To further characterize the functions of MIMP, we investigated its effects on the intestinal permeability, expression of tight junction (TJ) proteins and TJ ultrastructure in vitro and in vivo. We also determined the interaction between MIMP and dendritic cells (DCs). We observed that MIMP reduced intestinal permeability and restored the expression and distribution of TJ proteins in both NCM460 cell monolayers and in IL-10(-/-) mice. MIMP adhered to immature (i) DCs by binding to DC-SIGN, and induced DCs to produce anti-inflammatory cytokines and to mediate Th2 differentiation. Moreover, MIMP stimulated the expression of anti-inflammatory cytokines in colonic mucosa and attenuated colitis in IL-10(-/-) mice. In conclusion, MIMP is the main functional component of L. plantarum that contributes to its protective effects, and thus may be a potential therapeutic agent for intestinal diseases.

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Zhihua Liu, Tongyi Shen, Hongqi Chen, Yukun Zhou, Peng Zhang, Yanlei Ma, Mary Pat Moyer, Ming Zhang, Zhaoxin Chu, Huanlong Qin. Functional characterization of MIMP for its adhesion to the intestinal epithelium. Frontiers in Bioscience-Landmark. 2011. 16(6); 2106-2127.