Open Access
In vitro generation of immune cells from pluripotent stem cells
Bart Vandekerckhove1,Stijn Vanhee1,Stefanie Van Coppernolle1,Sylvia Snauwaert1,Imke Velghe1,Tom Taghon1,Georges Leclercq1,Tessa Kerre1,Jean Plum1
Department of Clinical Chemistry, University of Ghent, Ghent, Belgium.
DOI: 10.2741/3800 Volume 16 Issue 4, pp.1488-1504
Published: 01 January 2011
(This article belongs to the Special Issue Regenerative medicine immunity)

Stem cell transplant recipients and acquired or inherited immune-deficiency patients could benefit from the infusion of B, T and/or NK cells. These lymphoid cells can be generated in vitro from bone marrow derived CD34+CD45+ hematopoietic stem cells (HSC). The number of cells that can be obtained in this way is limited especially in the adult. An alternative source may therefore constitute human pluripotent stem cells (PSC) such as embryonic (hESC) or induced pluripotent stem cells (hiPSC). Here, we focus on present knowledge on the generation of lymphoid cells from hESC. The two main obstacles for the generation of clinically relevant immune cells are the failure to generate from hESC long-term repopulating HSC which could be kept in culture for prolonged time; and insufficient knowledge of the selection process which generates mature T cells from CD4 CD8 double positive (DP) precursors in vitro.

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Bart Vandekerckhove, Stijn Vanhee, Stefanie Van Coppernolle, Sylvia Snauwaert, Imke Velghe, Tom Taghon, Georges Leclercq, Tessa Kerre, Jean Plum. In vitro generation of immune cells from pluripotent stem cells. Frontiers in Bioscience-Landmark. 2011. 16(4); 1488-1504.