Open Access
Article
Regulation of resident and newcomer insulin granules by calcium and SNARE proteins
Kyota Aoyagi1,Mica Ohara-Imaizumi1,Shinya Nagamatsu1
1
Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan
DOI: 10.2741/3784 Volume 16 Issue 4, pp.1197-1210
Published: 01 January 2011
(This article belongs to the Special Issue New aspects of biphasic insulin secretion)
Abstract

Insulin, stored in large dense core granules, is biphasically exocytosed by glucose stimulation in pancreatic beta-cells. Several molecules, such as SNARE proteins, and Ca2+ ion are involved in the regulation of insulin exocytosis. Indeed, studies using gene targeting mice revealed critical roles of SNARE proteins and their accessory proteins, which may be associated with diabetes mellitus. In particular, the total internal reflection fluorescent (TIRF) imaging technique shed new light on the molecular mechanism of the insulin exocytotic process. In this review we discuss the mechanism of insulin exocytosis mainly from a point of view of imaging techniques.

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Kyota Aoyagi, Mica Ohara-Imaizumi, Shinya Nagamatsu. Regulation of resident and newcomer insulin granules by calcium and SNARE proteins. Frontiers in Bioscience-Landmark. 2011. 16(4); 1197-1210.