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Genetic modification of ex-vivo expanded stem cells for clinical application
Reeva Aggarwal1,Vincent J Pompili1,Hiranmoy Das1
1
Cardiovascular Stem Cell Research Laboratory, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
DOI: 10.2741/3650 Volume 15 Issue 3, pp.854-871
Published: 01 June 2010
(This article belongs to the Special Issue Stem cells in clinical medicine)
Abstract

Stem cell therapy is currently considered as an important regime for repairing, replacing or enhancing the biological functions of the damaged tissues. Among adult stem cells, hematopoietic stem cells (HSCs) are commonly used for cure of hematological disorders. However, the number of HSCs obtained from sources like bone marrow, peripheral or umbilical cord blood is not sufficient for routine clinical application. Thus, ex-vivo expansion of HSCs becomes critically important. Ex-vivo culture and expansion of stem cells are challenging, as stem cells differentiate in culture rather than self-renew. Lack of clarity about the factors responsible for quiescence and differentiation of HSCs, investigators struggled to optimize conditions for ex vivo expansion. As we understand better, various strategies can be incorporated to mimic in vivo conditions for successful expansion of stem cells. However, characterization and biological functionality should also be tested for expanded stem cells prior to clinical application. To treat ischemia by enhancing therapeutic angiogenesis and neo-vascularization, the role of genetic modification of HSCs with pro-angiogenic factors is the focus of this review.

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Reeva Aggarwal, Vincent J Pompili, Hiranmoy Das. Genetic modification of ex-vivo expanded stem cells for clinical application. Frontiers in Bioscience-Landmark. 2010. 15(3); 854-871.