Open Access
Article
EPC adhesion to arteries from diabetic and non-diabetic patients: effect of pioglitazone
Emilio Ruiz1,Santiago Redondo1,Antonio Gordillo-Moscoso1,Enrique Rodriguez1,Fernando Reguillo1,Jose Martinez-Gonzalez1,Teresa Tejerina1
1
Department of Pharmacology, School of Medicine, Universidad Complutense, 28040 Madrid, Spain
DOI: 10.2741/3475 Volume 14 Issue 9, pp.3608-3618
Published: 01 January 2009
Abstract

Endothelial progenitor cell (EPC) dysfunction is an important mediator of vascular disease in diabetes. We aimed to elucidate the mechanism of adhesion of EPC to diabetic and non-diabetic arteries and to study the effect of the anti-diabetic drug pioglitazone. Peripheral blood mononuclear cells were isolated from healthy donors. Human internal mammary arteries (HIMA) were isolated from patients who underwent coronary artery bypass surgery. EPC were labelled with 111In-oxine and perfused to HIMA in a perfusion chamber. Stromal derived factor-1 (SDF-1) and cyclooxygenase-2 (COX-2) were assessed by immunohistochemical analysis. CXCR-4 expression was assessed by flow cytometry. Adhesion of EPC was increased in HIMA from diabetic patients and was reduced after preincubation with 15 mM glucose for 72 h. EPC adhesion and CXCR-4 expression were inversely correlated. COX-2 and SDF-1 immunostaining in HIMA were positively correlated. Pioglitazone (1 microM) increased the adhesion of EPC to HIMA and the expression of CXCR-4 in EPC. Therefore, EPC-recruiting capability is increased in diabetic arteries, although EPC adhesion is notably impaired by high glucose concentrations. Interestingly, pioglitazone treatment enhances EPC adhesiveness.

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Emilio Ruiz, Santiago Redondo, Antonio Gordillo-Moscoso, Enrique Rodriguez, Fernando Reguillo, Jose Martinez-Gonzalez, Teresa Tejerina. EPC adhesion to arteries from diabetic and non-diabetic patients: effect of pioglitazone. Frontiers in Bioscience-Landmark. 2009. 14(9); 3608-3618.