Open Access
Proteomics strategies for target identification and biomarker discovery in cancer
Uros Rajcevic1,Simone P Niclou1,Connie R Jimenez1
Norlux Neuro-Oncology Laboratory, CRP-Sante, 84, Val Fleuri, L-1526 Luxembourg, Luxembourg.,
DOI: 10.2741/3452 Volume 14 Issue 9, pp.3292-3303
Published: 01 January 2009
(This article belongs to the Special Issue Tumor angiogenesis and molecular targets)

The revolution in genomics and proteomics has produced complex technologies enabling an insight into the functional effectors of cellular processes. In oncology these technologies lead to the identification of biological markers which may provide the starting point for the development and identification of diagnostic tests and therapeutic targets. To identify and validate reliable tumor markers within the proteome, it is necessary, prior to tandem mass spectrometry, to reduce sample complexity. This can be done by robust fractionation and separation techniques. This review addresses the discovery stage of onco-proteomics - the strategies for target identification and biomarker discovery in solid tumors and biofluids. The overview includes different proteomic methods, from gel-based to liquid chromatography (LC)-based separations of proteins/peptides, and the corresponding detection by mass spectrometry. The quantitative methods in mass spectrometry include techniques based on stable isotope labeling of proteins/peptides and label-free methods. A particular emphasis is given to proteomics-based biomarker discovery in biofluids (e.g. plasma, urine, secretome, cerebrospinal fluid) and target identification in tissue for anti-angiogenic therapies.

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Uros Rajcevic, Simone P Niclou, Connie R Jimenez. Proteomics strategies for target identification and biomarker discovery in cancer. Frontiers in Bioscience-Landmark. 2009. 14(9); 3292-3303.