Open Access
Vision tests in the mouse: Functional phenotyping with electroretinography
Naoyuki Tanimoto1,Regine L Muehlfriedel1,M Dominik Fischer1,Edda Fahl1,Peter Humphries1,Martin Biel1,Mathias W Seeliger1
Ocular Neurodegeneration Research Group, Centre for Ophthalmology, Institute for Ophthalmic Research, University of Tuebingen, Schleichstrasse 4/3, D-72076 Tuebingen, Germany.
DOI: 10.2741/3409 Volume 14 Issue 7, pp.2730-2737
Published: 01 January 2009
(This article belongs to the Special Issue Genetics of vision and cognition)

Electroretinography (ERG) is an established diagnostic technique in clinical ophthalmology and provides objective information about retinal function. This technique is also applied in basic research, where animal models of hereditary retinopathies have significantly contributed to our understanding of the composition of ERG responses in general and how retinal degenerative pathologies alter retinal function specifically. Indeed, electrophysiologic assessment of transgenic mice, which are genetically engineered to mimic human mutations that lead to retinal diseases, can be well compared with clinical data. Furthermore, limitations on examinations (e.g. length of measurement, range of light intensity) are much less of a concern when assessing mice compared to human patients. In order to measure and analyze retinal responses properly, several important aspects have to be considered. This paper focuses on these aspects, and shows exemplary ERG data which were obtained from normal wild-type mice and from transgenic mice with specific functional properties, namely Rho-/- (rod opsin knockout, cone function only), and Cnga3-/- (cone CNG channel deficient, rod function only) to illustrate rod and cone system contributions to ERG responses.

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Naoyuki Tanimoto, Regine L Muehlfriedel, M Dominik Fischer, Edda Fahl, Peter Humphries, Martin Biel, Mathias W Seeliger. Vision tests in the mouse: Functional phenotyping with electroretinography. Frontiers in Bioscience-Landmark. 2009. 14(7); 2730-2737.