Open Access
Article
Combined microPET/CT for imaging of hepatocellular carcinoma in mice
Christian von Falck1,Thomas Rodt1,Roman Halter1,Reinhard Spanel1,Michael Galanski1,Juergen Borlak1
1
Institute of Radiology, Hannover Medical School, Hannover, Germany. c.v.falck@gmx.de
DOI: 10.2741/3371 Volume 14 Issue 6, pp.2193-2202
Published: 01 January 2009
(This article belongs to the Special Issue Molecular imaging in cancer)
Abstract

The EGF-transgenic mouse is a genetic model of hepatocellular carcinoma that allows for a comprehensive study of signal pathways, molecular interactions and the evaluation of novel therapeutic concepts. In this regard, non-invasive imaging tools for serial in-vivo monitoring of tumor load and growth are highly desirable. This study therefore aimed at demonstrating the feasibility of non-invasive in-vivo imaging of primary liver malignancies in mice using combined contrast-enhanced microCT and F-18 FDG microPET. In our murine disease model, microCT enabled imaging of primary liver tumors down to a lesional diameter of 0.9 mm. F-18 FDG tumor-to-non-tumor ratio of HCCs was observed to be dependent on lesion size and linked to overpression of glucose transporters and hexokinase isoenzymes as determined by gene expression studies. Histopathologic analyses indicated an increased cellular dedifferentiation with increase lesion size, as well.

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Christian von Falck, Thomas Rodt, Roman Halter, Reinhard Spanel, Michael Galanski, Juergen Borlak. Combined microPET/CT for imaging of hepatocellular carcinoma in mice. Frontiers in Bioscience-Landmark. 2009. 14(6); 2193-2202.