Open Access
Overexpression of hypoxia/inflammatory markers in atherosclerotic carotid plaques
Ana Luque1,Marta Turu1,Oriol Juan-Babot1,Pere Cardona1,Angels Font1,Ana Carvajal1,Mark Slevin1,Elena Iborra1,Francisco Rubio1,Lina Badimon1,Jerzy Krupinski1
Department of Neurology, Stroke Unit, University Hospital of Bellvitge and IDIBELL, Barcelona, Spain
DOI: 10.2741/3168 Volume 13 Issue 17, pp.6483-6490
Published: 01 May 2008

Hypoxia, angiogenesis and inflammation leads to plaque progression and remodelling and may significantly contribute towards plaque rupture and subsequent cerebrovascular events. Our aim was to study, markers of hypoxia and inflammation previously identified by microarray analysis, in atherosclerotic carotid arteries with low to moderate stenosis. We hoped to describe different cellular populations expressing the studied markers. The location of selected inflammatory molecules obtained as vascular transplants from organ donors were analysed by immunohistochemistry with monoclonal and polyclonal antibodies. Paraffin-embedded sections were cut and probed with antibodies recognizing active B and T-lymphocytes (CD30), hypoxia-inducible factor-1alpha, endoglin (CD105), Interleukin-6 and C-reactive protein. We observed a notable overexpression of HIF-1alpha in inflammatory and hypoxic areas of carotid arteries in all types of lesions from type II-V taken from the patients with carotid stenosis less than 50%. This suggests that HIF-1alpha may have a putative role in atherosclerosis progression and angiogenesis. Dynamic changes in the non-occluding plaques may explain some of the clinical events in patients with low to moderate carotid stenosis.

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Ana Luque, Marta Turu, Oriol Juan-Babot, Pere Cardona, Angels Font, Ana Carvajal, Mark Slevin, Elena Iborra, Francisco Rubio, Lina Badimon, Jerzy Krupinski. Overexpression of hypoxia/inflammatory markers in atherosclerotic carotid plaques. Frontiers in Bioscience-Landmark. 2008. 13(17); 6483-6490.