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Multiple signaling is involved in endostatin-mediated apoptosis in ECV 304 endothelial cells
Bin Ren1,Yuan Wang1,Kenneth Ndebele1,Qiang Zhi1,Fei-Hu Chen1,Yu-Zhen Wang1,Sareh Parangi1
1
School of Life Science, University of Science and Technology of China, Hefei 230027, China. bin_ren@hms.harvard.edu <bin_ren@hms.harvard.edu>
DOI: 10.2741/1602 Volume 10 Issue 2, pp.1089-1097
Published: 01 May 2005
(This article belongs to the Special Issue Tumor growth and metastasis: molecular signaling and therapy)
Abstract

Apoptosis of vascular endothelial cells is associated with the regression of angiogenesis. Endostatin is a potential anti-angiogenic drug, but the effects of endostatin on apoptotic machinery in endothelial cells largely remain unclear. In the present study, human endostatin was expressed in E. Coli to induce apoptosis in endothelial cells. It was found that the expressed human endostatin specifically affected the viability of the ECV 304 in a dose-dependent manner. Endostatin induced apoptosis in these cells in a caspase-dependent manner, and endostatin-mediated apoptosis is associated with several apoptotic signaling pathways including overloading of intracellular magnesium and calcium, as well as regulation of p53 and Bcl 2 expression.

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Bin Ren, Yuan Wang, Kenneth Ndebele, Qiang Zhi, Fei-Hu Chen, Yu-Zhen Wang, Sareh Parangi. Multiple signaling is involved in endostatin-mediated apoptosis in ECV 304 endothelial cells. Frontiers in Bioscience-Landmark. 2005. 10(2); 1089-1097.