Open Access
Fluorometric evidence for different stoichiometries for the Na+/Mg2+ exchange in Mg-loaded rat thymocytes
Constanza Contreras-Jurado1,Marco T González-Martínez1,Enrique J Cobos1,Agatángelo Soler-Diaz1
Departamento de Fisiología, Facultad de Medicina. Universidad de Granada, Avda Madrid 13 E-18012 Granada, Spain
DOI: 10.2741/1274 Volume 9 Issue 2, pp.1843-1848
Published: 01 May 2004
(This article belongs to the Special Issue Magnesium and cell proliferation and differentiation)

The regulation of the cytosolic free magnesium concentration ([Mg2+]i) is a fundamental cellular process that requires magnesium extruding mechanisms. Here, we present evidence indicating that rat thymocytes are endowed with different Na/Mg exchange systems. Fluxes of magnesium were measured using the fluorescent magnesium indicator magfura-2. Cells were loaded with magnesium using the calcium ionophore A-23187 to 0.6-8.0 mM [Mg2+]i (resting [Mg2+]i = 0.38 +/- 0.06 mM, n = 5). The presence of extracellular sodium was required for magnesium exit. The initial rate of [Mg2+]i was stimulated by extracellular sodium with Michaelis-Menten kinetics. The Vmax of the sodium-dependent magnesium exit was markedly increased by [Mg2+]i. Holding the membrane potential at either -84 mV or at -10 mV had different effects on the sodium-stimulated magnesium efflux, depending on the extracellular sodium concentration ([Na+]o). At 10-30 mM [Na+]o, the magnesium efflux was faster at -10 mV than at -84 mV. Conversely, at 50-200 mM [Na+]o, the efflux of magnesium was faster at -84 mV that at -10 mV. At 75 mM [Na+]o, the activities where nearly the same at both membrane potential values. These observations suggest that the stochiometry of the Na+/Mg2+ exchange changes with [Na+]o.

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Constanza Contreras-Jurado, Marco T González-Martínez, Enrique J Cobos, Agatángelo Soler-Diaz. Fluorometric evidence for different stoichiometries for the Na+/Mg2+ exchange in Mg-loaded rat thymocytes. Frontiers in Bioscience-Landmark. 2004. 9(2); 1843-1848.