Open Access
Modifications of tyrosine and catecholamines by peroxynitrite, nitrite and nitrate
Wu-Nan Kuo1,Joseph M Kocis1,Luvina K Guruvadoo1
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114, USA.
DOI: 10.2741/1118 Volume 8 Issue 1, pp.139-142
Published: 01 May 2003
(This article belongs to the Special Issue Nitric oxide and related research)

At pH 7.0 in non-"deaired" potassium phosphate buffer, the reactions of L-tyrosine, L-dopa, dopamine, L-norepinephrine, and L-epinephrine with peroxynitrite (PN) or nitrite, generated colored products. These products displayed not only unique colors and patterns of mobility on silica thin layer chromatographic plates, but also varied increase of absorbance between 400 and 540 nm. In particular, with the treatment of nitrite, catecholamines exhibited longer bands and multiple colored spots due to the formation of multiple compounds. In addition, significantly increased mobilities were noted with nitrate-incubated catecholamines. These results imply the occurrence of various types of reactions, such as nitration and nitrosation, via the production of active intermediates of oxygen and/or nitrogen species during incubation.

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Wu-Nan Kuo, Joseph M Kocis, Luvina K Guruvadoo. Modifications of tyrosine and catecholamines by peroxynitrite, nitrite and nitrate. Frontiers in Bioscience-Landmark. 2003. 8(1); 139-142.