Open Access
Modulation of protein kinase C (PKC)-mediated contraction and the possible role of PKC epsilon in rat mesenteric arteries
Yuichi Shirasawa1,Travis J Rutland1,Jennifer L Young1,David A Dean1,Benoit N Joseph1
Department of Pharmacology, Physiology and Therapeutics, University of North Dakota, Grand Forks, North Dakota 58202, USA
DOI: 10.2741/1087 Volume 8 Issue 1, pp.133-138
Published: 01 May 2003
(This article belongs to the Special Issue Microcirculation)

he involvement of protein kinase C (PKC) in isometric tension development of rat mesenteric arteries was investigated. Non-selective inhibition of PKC and selective inhibition of the epsilon isoform were performed using the PKC inhibitor, chelerythrine, and non-viral gene-transfer of a kinase inactive mutant of PKCepsilon (PKCepsilon-KN), respectively. Chelerythrine (2.5 or 5.0 microM) significantly and equally attenuated phenylephrine-induced but not potassium-induced contractions. Higher concentrations of chelerythrine (10 microM) caused the vessels to lose responsiveness to both phenylephrine and potassium chloride. Transfection of blood vessels with epsilon-KN also resulted in significant attenuation of contractile responses to phenylephrine. Potassium chloride-induced responses were not altered in transfected arteries. In a separate group of vessels, the relationship between [Ca2+]i and isometric tension was evaluated. These studies suggested that calcium sensitivity of the contractile apparatus was decreased in vessels when PKC-epsilon activity was compromised. The results of the study suggest that PKC-epsilon can modulate phenylephrine-induced contraction in mesenteric arteries via calcium-independent pathways.

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Yuichi Shirasawa, Travis J Rutland, Jennifer L Young, David A Dean, Benoit N Joseph. Modulation of protein kinase C (PKC)-mediated contraction and the possible role of PKC epsilon in rat mesenteric arteries. Frontiers in Bioscience-Landmark. 2003. 8(1); 133-138.