Open Access
Mitochondrial DNA damage analysis in bronchoalveolar lavage cells of preterm infants
Bea Zoer1,Jasper Valentijn Been1,Eveline Jongen1,Anne Debeer1,Alexandra Hendrickx1,Hubertus J Smeets1,Luc Jacques Zimmermann1,Eduardo Villamor1
Department of Pediatrics Maastricht University Medical Center, GROW School for Oncology and Developmental Biology, 6202 AZ Maastricht, The Netherlands
DOI: 10.2741/E96 Volume 2 Issue 1, pp.361-368
Published: 01 January 2010
(This article belongs to the Special Issue Biochemical markers in biological fluids)

In mechanically ventilated preterm infants, the combination of immaturity, volutrauma, oxidative stress, and inflammatory processes can lead to chronic lung injury. Mitochondrial DNA (mtDNA) is more susceptible to oxidative damage than nuclear DNA. We aimed to investigate the level of mtDNA damage (deletions, mutations and changes in copy number) in bronchoalveolar lavage (BAL) cells from 10 preterm infants (27-30 weeks). A first BAL (BAL1) was done within 24 h of endotracheal intubation and BAL2 was performed 30-103 h thereafter. Deletions were analyzed by long range PCR, point mutations by heteroduplex analysis of the D-loop region, and copy number changes by real-time PCR. Using these methods, no deletions were found in any of the BAL samples. When BAL1 and BAL2 samples were compared no new mutations were found. In contrast, a marked decrease in mtDNA copy number was observed in 5 patients. In conclusion, we found that exposure of preterm infants to short term mechanical ventilation did not lead to detrimental consequences for the mtDNA in the form of mutations or deletions.

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Bea Zoer, Jasper Valentijn Been, Eveline Jongen, Anne Debeer, Alexandra Hendrickx, Hubertus J Smeets, Luc Jacques Zimmermann, Eduardo Villamor. Mitochondrial DNA damage analysis in bronchoalveolar lavage cells of preterm infants. Frontiers in Bioscience-Elite. 2010. 2(1); 361-368.