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Reproducibility of measuring cerebral blood flow by laser-Doppler flowmetry in mice
Yosuke Tajima1,2,Hiroyuki Takuwa1,Hiroshi Kawaguchi1,Kazuto Masamoto1,3,Yoko Ikoma1,Chie Seki1,Junko Taniguchi1,Iwao Kanno1, Naokatsu Saeki2,Hiroshi Ito1,*
1
Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan
2
Department of Neurological Surgery, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuoku, Chiba 260-8670, Japan
3
Center for Frontier Science and Engineering, University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan
DOI: 10.2741/E691 Volume 6 Issue 1, pp.62-68
Published: 01 January 2014
(This article belongs to the Special Issue Brain activation and cerebral blood flow regulation)
*Corresponding Author(s):  
Hiroshi Ito
E-mail:  
hito@nirs.go.jp
Abstract

Laser-Doppler flowmetry has been widely used to trace hemodynamic changes in experimental stroke research. The purpose of the present study was to evaluate the day-to-day test-retest reproducibility of measuring cerebral blood flow by LDF in awake mice. The flux indicating cerebral blood flow (CBF), red blood cell (RBC) velocity, and RBC concentration were measured with LDF via cranial windows for the bilateral somatosensory cortex in awake mice. LDF measurements were performed three times, at baseline, 1 hour after, and 7 days after the baseline measurement. Moreover, breathing rate (BR) and partial pressure of transcutaneous CO₂ (PtCO₂) were measured simultaneously with LDF measurement. Intraclass correlation coefficient (ICC) and within-subject coefficient of variation (CVw) were calculated. CBF, RBC velocity, and RBC concentration showed good day-to-day test-retest reproducibility (ICC: 0.61 - 0.95, CVw: 8.3% - 15.4%). BR and PtCO₂ in awake mice were stable during the course of the experiments. The evaluation of cerebral microcirculation using LDF appears to be applicable to long-term studies.

Key words

Laser-Doppler Flowmetry, Cerebral Blood Flow, Test-retest Reproducibility, Intraclass Correlation Coefficient, Within-subject Coefficient of Variation

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Yosuke Tajima, Hiroyuki Takuwa, Hiroshi Kawaguchi, Kazuto Masamoto, Yoko Ikoma, Chie Seki, Junko Taniguchi, Iwao Kanno, Naokatsu Saeki, Hiroshi Ito. Reproducibility of measuring cerebral blood flow by laser-Doppler flowmetry in mice. Frontiers in Bioscience-Elite. 2014. 6(1); 62-68.