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Nuclear localization of ISWI ATPase Smarca5 (Snf2h) in mouse
Jarmila Vargova1,Karina Vargova1,Arthur I Skoultchi1,Tomas Stopka1
1
Pathological Physiology and Center of Experimental Hematology, First Faculty of Medicine, Charles University, Prague, Czech Republic
DOI: 10.2741/E53 Volume 1 Issue 2, pp.553-559
Published: 01 June 2009
Abstract

Nucleosome movement is, at least in part, facilitated by ISWI ATPase Smarca5 (Snf2h). Smarca5 gene inactivation in mouse demonstrated its requirement at blastocyst stage; however its role at later stages is not completely understood. We herein determined nuclear distribution of Smarca5 and histone marks associated with actively transcribed and repressed chromatin structure in embryonic and adult murine tissues and in tumor cells. Confocal microscopy images demonstrate that Smarca5 is localized mainly in euchromatin and to lesser extent also in heterochromatin and nucleoli. Smarca5 heterozygous mice for a null allele display decreased levels of histone H3 modifications and defects in heterochromatin foci supporting role of Smarca5 as a key regulator of global chromatin structure.

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Jarmila Vargova, Karina Vargova, Arthur I Skoultchi, Tomas Stopka. Nuclear localization of ISWI ATPase Smarca5 (Snf2h) in mouse. Frontiers in Bioscience-Elite. 2009. 1(2); 553-559.