Open Access
The role of nuclear factor-kappa-B p50 subunit in the development of endometriosis
Yuan Lu1,Qunyan Sun1,Yu Zheng1,Xishi Liu1,Jian-Guo Geng1,Sun-Wei Guo1
Shanghai Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China
DOI: 10.2741/E272 Volume 3 Issue 2, pp.591-603
Published: 01 January 2011
(This article belongs to the Special Issue Frontiers in endometriosis)

p50 is a member of the NF-kappaB family known to be involved in endometriosis. To gain insight into the roles of p50 in the development of endometriosis, we cross-transplanted endometrial fragments from p50 knockout mice to wild-type mice and vice versa, and also autotransplanted the fragments within the knockout and wild-type mice, inducing endometriosis. We then evaluated the size of the endometrial implants, and immunoreactivity to phosphorylated p65 (p-p65), PKCepsilon and TRPV1 in ectopic and eutopic endometrium as well as in vagina. We found that p50 deletion significantly reduces the size of endometrial implants. The immunoreactivity to p-p65 and PKCepsilon, but not TRPV1, was reduced in endometrial implants in p50 knockout mice. Deletion of p50 significantly reduced p-p65 and PKCepsilon, but not TRPV1, expression in eutopic endometrium and vagina. It also disrupts NF-kappaB activation and PKCepsilon expression in eutopic and vagina, suggesting the role of NF-kappaB in regulating PKCepsilon, which plays an important role in nociception. These data show that p50 is involved in the development of endometriosis and may be a promising therapeutic target.

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Yuan Lu, Qunyan Sun, Yu Zheng, Xishi Liu, Jian-Guo Geng, Sun-Wei Guo. The role of nuclear factor-kappa-B p50 subunit in the development of endometriosis. Frontiers in Bioscience-Elite. 2011. 3(2); 591-603.