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Scutellariae radix suppresses hepatitis B virus production in human hepatoma cells
Ya Ping Tseng1,Yi Chieh Wu1,Yann Lii Leu1,Sheau Farn Yeh1,Chen Kung Chou1
1
Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC
DOI: 10.2741/E213 Volume 2 Issue 4, pp.1538-1547
Published: 01 June 2010
(This article belongs to the Special Issue Molecular biology and pathogenesis of HBV and HDV)
Abstract

The traditional Chinese medicine Xiao-Chai-Hu-Tang (HD-7) has been widely used to treat liver diseases in China and Japan. HD-7 consists of seven different ingredients, but which one provides the therapeutic benefits is still unknown. Here, we identified the "Minister herb" Scutellariae radix (HD-1S), but not the "King herb" Bupleuri radix (HD-1B) in HD-7, as the active component that suppresses HBV gene expression and virus production in human hepatoma cells. We have found that an aqueous extract of HD-1S not only suppressed wild-type virus but also lamivudine-resistant HBV mutant in human hepatoma cells. We show that HD-1S selectively suppresses HBV core promoter activity. Electrophoretic mobility shift assay analysis has revealed that HD-1S treatment decreases the DNA-binding activity of nuclear extract of HepA2 cells to a specific cis-element of the HBV core promoter, which includes the peroxisome proliferator-activated receptor binding site and HNF4. Furthermore, ectopic expression of PGC-1 abolished the suppression of HD-1S on HBV core promoter activity suggesting that HD-1S may act through modulating hepatic transcriptional machinery to suppress HBV viral gene expression and virus production.

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Ya Ping Tseng, Yi Chieh Wu, Yann Lii Leu, Sheau Farn Yeh, Chen Kung Chou. Scutellariae radix suppresses hepatitis B virus production in human hepatoma cells. Frontiers in Bioscience-Elite. 2010. 2(4); 1538-1547.