Open Access
Species specificity of prorenin binding to the (pro)renin receptor in vitro
Kazal Boron Biswas1,A H M Nurun Nabi1,Yoshie Arai1,Tsutomu Nakagawa1,Akio Ebihara1,Fumiaki Suzuki1
United Graduate School of Agricultural Science, Gifu University, Gifu 501-1193, Japan
DOI: 10.2741/E183 Volume 2 Issue 4, pp.1234-1240
Published: 01 June 2010
(This article belongs to the Special Issue Handle region peptide and prorenin receptor)

In this study, binding properties of human prorenin to rat (pro)renin receptor (r(P)RR) and rat prorenin to human (pro)renin receptor (h(P)RR) were elucidated in vitro to investigate species specificity of prorenin bindings to (P)RR. Both (P)RRs were expressed in vitro based on wheat germ lysate and purified using His trap column. The association and dissociation rate constants of human and rat prorenin for the immobilized r(P)RR and h(P)RR were 6.64 x 10(6) and 1.01 x 10(6) M-1.s-1 and, 0.024 and 8.45 x 10(-3) s-1, respectively. Their KD values were 3.7 nM (3-fold higher than that of human prorenin vs h(P)RR (1.2 nM)) and 8.3 nM (1.2-fold lower than that of rat prorenin vs r(P)RR (10.2 nM)), respectively. Additionally, human and rat prorenin bound to the pre-adsorbed rat and human (P)RR, respectively, performed enzymatic activities. Their molecular activities were 4.1h-1 (almost similar (3.8h -1) to human prorenin vs h(P)RR) and 0.98 h-1 (approximately 2-fold lower than rat prorenin vs r(P)RR), respectively. Thus, these results suggest the species specificity for bindings of prorenin to (P)RR, which could be useful in elucidating the biochemical properties of prorenin binding to the receptor.

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Kazal Boron Biswas, A H M Nurun Nabi, Yoshie Arai, Tsutomu Nakagawa, Akio Ebihara, Fumiaki Suzuki. Species specificity of prorenin binding to the (pro)renin receptor in vitro. Frontiers in Bioscience-Elite. 2010. 2(4); 1234-1240.