Open Access
Article
Proteomics reveals high levels of vitamin D binding protein in myocardial infarction
Cosimo Gasparri1,Antonio Curcio1,Daniele Torella1,Marco Gaspari1,Vittoria Celi1,Francesco Salituri1,Duino Boncompagni1,Michele Torella1,Elio Gulletta1,Giovanni Cuda1,Ciro Indolfi1
1
Division of Cardiology, Magna Graecia University, Catanzaro, Italy
DOI: 10.2741/E140 Volume 2 Issue 3, pp.796-804
Published: 01 June 2010
(This article belongs to the Special Issue Biochemical markers in biological fluids)
Abstract

The pathogenic mechanisms underlying cardiovascular diseases involve significant alterations in myocardial gene and protein expression. Proteomics analysis can define new protein and peptide changes associated with cardiac pathology, including myocardial infarction. The aim of the present study was to analyze serum proteome of patients with ST-Elevation myocardial infarction (STEMI). Serum samples were collected from STEMI patients (age 65.0+/-10.3) at 5.3+/-2.7 hours after the onset of typical chest pain and before initiating standard therapy. Ten age- and sex-matched donors were used as controls. The samples were albumin- and IgG-depleted. Isotope-coded affinity tag method was employed to label cysteine residues and liquid chromatography-Tandem Mass Spectrometry analysis was performed to measure the labeled proteins. Our proteomic approach identified increased levels of vitamin D-binding protein precursor (VDB) in the serum of STEMI patients when compared to control donors. Western blot analysis confirmed the increase in VDB protein in STEMI patients. Moreover, fresh thrombotic plaques, obtained during primary angioplasty, showed high expression of VDB protein. Mechanistically, VDB protein reduces platelet aggregation and prolongs coagulation time ex vivo.

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Cosimo Gasparri, Antonio Curcio, Daniele Torella, Marco Gaspari, Vittoria Celi, Francesco Salituri, Duino Boncompagni, Michele Torella, Elio Gulletta, Giovanni Cuda, Ciro Indolfi. Proteomics reveals high levels of vitamin D binding protein in myocardial infarction. Frontiers in Bioscience-Elite. 2010. 2(3); 796-804.