Open Access
Article
Beta-cyclodextrin in DNA decompaction: an imaging approach
Alfredo Gonzalez-Perez1
1
Department of Physics and Chemistry, University of Southern Denmark, MEMPHYS - Center for Biomembrane Physics, Campusvej 55, DK-5230 Odense M, Denmark. alfredo@memphys.sdu.dk
DOI: 10.2741/E128 Volume 2 Issue 2, pp.684-693
Published: 01 January 2010
(This article belongs to the Special Issue Bioorganic and biophysical chemistry: from basics to applications)
Abstract

In the present work we investigate the CTAB:DNA complexes decompaction process using beta-CD as a decompacting agent. The transition from globules (compacted DNA) to coils (decompacted DNA) was achieved without a coexistence region between coils and globules. This non first-order transition was investigated combining fluorescence microscopy (FM), cryo-transmission electron microscopy (cryo-TEM) and TEM of negatively stained samples. Additionally the presence of multi-globular aggregates in the proximity of the critical transition was elucidated. A possible mechanism for the decompaction process was suggested.

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Alfredo Gonzalez-Perez. Beta-cyclodextrin in DNA decompaction: an imaging approach. Frontiers in Bioscience-Elite. 2010. 2(2); 684-693.