Open Access
A novel testis-specific Na+/H+ exchanger is involved in sperm motility and fertility
Tao Liu1,Jun-Cheng Huang1,Wu-Lin Zuo1,Cui-Ling Lu1,Min Chen1,Xue-Sen Zhang1,Yi-Chuan Li1,Huan Cai1,Wen-Liang Zhou1,Zhao-Yuan Hu1,Fei Gao1,Yi-Xun Liu1
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
DOI: 10.2741/E115 Volume 2 Issue 2, pp.566-581
Published: 01 January 2010
(This article belongs to the Special Issue Advances in mammalian reproduction)

Sodium-hydrogen exchanger as a channel for regulation of intracellular pH might be a crucial modulator of sperm capacitation and motility. Three members of this family have been identified in spermatozoa. A novel protein testis-specific sodium-hydrogen exchanger named mtsNHE was cloned in the present study. The mtsNHE localizing on principle piece of sperm flagellum contained 12 predicted transmembrane regions without cytoplasmic fragment at carboxyl terminus. Hydrophilic region was common in the sodium-hydrogen exchanger family members. Polyclonal antibodies to trans-membrane region significantly reduced sperm motility, acrosome reaction and ratio of in vitro fertilization. By in-pouring the antibodies in sperm solution, intracellular pH and calcium concentration were decreased. Muscle injection of female mice with the specific gene vaccine of mtsNHE, significantly stepped down fertility rate. Considering its specific expression and involvement in the regulation of fertility, the mtsNHE might be a potential target molecule for developing a new male contraceptive.

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Tao Liu, Jun-Cheng Huang, Wu-Lin Zuo, Cui-Ling Lu, Min Chen, Xue-Sen Zhang, Yi-Chuan Li, Huan Cai, Wen-Liang Zhou, Zhao-Yuan Hu, Fei Gao, Yi-Xun Liu. A novel testis-specific Na+/H+ exchanger is involved in sperm motility and fertility. Frontiers in Bioscience-Elite. 2010. 2(2); 566-581.