Open Access
Article
Expression of VEGF, VEGF-C and VEGFR-2 in in situ and invasive SCC of cervix
Robert Jach1,Joanna Dulinska-Litewka1,Piotr Laidler1,Andrzej Szczudrawa1,Andrzej Kopera1,Lukasz Szczudlik1,Michal Pawlik1,Krzysztof Zajac1,Monika Mak1,Antoni Basta1
1
Department of Gynecology, Obstetrics and Oncology, Jagiellonian University, Medical College, Krakow, Poland. jach@cm-uj.krakow.pl
DOI: 10.2741/E101 Volume 2 Issue 2, pp.411-423
Published: 01 January 2010
Abstract

Cervical squamous cell carcinoma (SCC) arises from the metaplastic epithelium and develops slowly through dysplastic changes (i.e., cervical intraepithelial neoplasia--CIN) to carcinoma in situ and invasive cancer. There is little data concerning the quantitation of vascular endothelial growth factor (VEGF) and its correlation to the clinical or pathologic characteristics of SCC. This study assessed the expression of VEGF, VEGF-C and their receptor VEGFR-2 in 35 samples of normal cervical tissue, 35--CIN1, 35--CIN2 (25 non-pregnant, 15 pregnant women), 35--CIN3 and 30- SCC. VEGF, VEGF-C and VEGFR-2 were analyzed using RT-PCR, RQ-PCR, immunohistochemical staining and Western blot. VEGF, VEGF-C and VEGFR-2 were not detected in normal cervical epithelium. In CIN and SCC, both forms of VEGF and its receptor were identified, indicating a correlation between the increasing expression and staging of carcinoma. Results show the important role of VEGF in cervical progression and that the switch to the lymphangiogenesis phenotype occurs prior to the stage of invasion likely at CIN2/3.

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Robert Jach, Joanna Dulinska-Litewka, Piotr Laidler, Andrzej Szczudrawa, Andrzej Kopera, Lukasz Szczudlik, Michal Pawlik, Krzysztof Zajac, Monika Mak, Antoni Basta. Expression of VEGF, VEGF-C and VEGFR-2 in in situ and invasive SCC of cervix. Frontiers in Bioscience-Elite. 2010. 2(2); 411-423.